LOCUS       sequence-240439-       11059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; 
            QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11059)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-001428-QES.c10.L544Y
FEATURES             Location/Qualifiers
     source          1..11059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(308..326)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        394..498
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(499..567,568..1359)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(717..736)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1530..2118
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2019..2038
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2549..2694
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2737..3774
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3772..3791)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3816..3864
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4518..4897
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4898..5101
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5052..5072
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5098..5122
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7761..7895
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7785..7804)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7839..7858
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8038..8060)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8620..10409
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 atccaggtct gaaaattccc catcctccga accatcctcg tcctcatcac caattactcg
       61 cagcccggaa aactcccgct gaacatcctc aagatttgcg tcctgagcct caagccaggc
      121 ctcaaattcc tcgtccccct ttttgctgga cggtagggat ggggattctc gggacccctc
      181 ctcttcctct tcaaggtcac cagacagaga tgctactggg gcaacggaag aaaagctggg
      241 tgcggcctgt gaggatcagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga
      301 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      361 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      421 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      481 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      541 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
      601 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
      661 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
      721 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
      781 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
      841 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
      901 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
      961 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     1021 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     1081 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     1141 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     1201 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     1261 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     1321 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     1381 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1441 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1501 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1561 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1621 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     1681 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1741 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1801 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1861 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1921 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1981 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2041 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2101 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2161 tggccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac
     2221 gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct
     2281 cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccccgagatg cgccgcgtgc
     2341 ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca
     2401 cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt
     2461 gccgccctgc ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga
     2521 aatatatttg catgtcttta gttctatgat gacacaaacc ccgcccagcg tcttgtcatt
     2581 ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat
     2641 taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt
     2701 caacagcgtg ccgcagatcc cggggggcaa tgagatatga aaaagcctga actcaccgcg
     2761 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     2821 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     2881 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     2941 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     3001 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     3061 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     3121 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     3181 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     3241 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     3301 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     3361 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     3421 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     3481 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     3541 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     3601 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     3661 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     3721 ggaaaccgac gccccagcac tcgtccggat cgggagatgg gggaggctaa ctgaaacacg
     3781 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     3841 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     3901 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     3961 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     4021 ctgccatagc cactggcccc gtgggttagg gacggggtcc cccatgggga atggtttatg
     4081 gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgact ggactgagca
     4141 gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc gcgacacgaa
     4201 caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc gcggatttct
     4261 ggcgtgccaa gctagtcgac caattctcat gtttgacagc ttatcatcgc agatccgggc
     4321 aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct atacattgaa
     4381 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
     4441 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
     4501 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
     4561 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     4621 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     4681 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     4741 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
     4801 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
     4861 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
     4921 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     4981 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
     5041 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     5101 tcgtttagtg aaccgtcaga tctctagaag ctgggtacca gctgctagcg ccaccatgcc
     5161 tatggggagc ctgcaaccgt tggccacgtt gtacctcctg ggcatgctcg tagcaagtgt
     5221 gctggcagtc gagaatctct gggtaaccgt ttactatggg gttcccgtgt ggaaggaggc
     5281 gcgcacaact ttgttttgcg cgtctgatgc gaaggcctac gagaccgaag tccataacgt
     5341 gtgggcaacc cacgcttgcg tacctacgga ccctaacccc caagagatgg tgttgggaaa
     5401 cgtcacggaa aacttcaaca tgtggaagaa tgatatggta gaccagatgc acgaggacgt
     5461 aatttctttg tgggcacaaa gtctcaagcc ttgtgttaaa ctgacgccac tttgcgtaac
     5521 cttggaatgc acacaggtca acgcgactca ggggaataca actcaggtaa acgtcactca
     5581 ggtcaacggc gatgaaatga agaattgcag tttcaacaca acgaccgaaa tcagagacaa
     5641 gaaacaaaaa gcgtatgcac tgttctatcg actggacctc gttccgttgg agagagagaa
     5701 cagaggagat tcaaattccg catctaagta cattctcatc aattgtaaca cctctgccat
     5761 cacgcaggcg tgcccaaaaa tgaatttcga ccctatccca atacattact gcaccccggc
     5821 aggctatgca atactcaaat gcaataataa gacatttaac ggcacaggtt cttgcaacaa
     5881 cgttagtaca gtgcaatgca ctcacgggat taagcccact atgagcacac aactgctgct
     5941 taatggttca ttggccgaag aggagattat cattagaagc gaaaacctca ccgataatgt
     6001 aaagaccatc attgtccacc tggatcagag cgtggagatc gtgtgtacta gaccgaataa
     6061 caacacccgg aagagtatca gaatcgggcc aggccagaca ttctatgcta ctggagacat
     6121 aatcggaaac attcgagagg cccactgcaa catctccgaa aaaaagtggc atgaaatgct
     6181 gaggagagtg tctgaaaagc ttgcggagca ctttccaaac aagaccatca aatttacttc
     6241 tagcagtggt ggcgacttgg agataacgac tcatagtttt aactgcagag gagaattttt
     6301 ttactgcaat acgtctgggc ttttcaactc cacatatatg ccgaacggaa catatatgcc
     6361 caatggcacg aataacagca attccacgat aattctgccc tgtagaataa agcaaattat
     6421 aaatatgtgg caagaggtag ggagagcgat gtatgctcca ccaatagctg gaaacattac
     6481 ttgtaattct aatataaccg ggctcttgct cgtgagggac ggaggaaaaa ataacaacac
     6541 tgagatattc cgccccggcg ggggggatat gcgggacaac tggagaagcg aactttataa
     6601 gtacaaggtc gttgagatta aacccctggg cgtcgccccg acgagggcga agcgacgggt
     6661 tgttgagagg gaaaaacgag cggtcggcct cggcgccgtt ttcctcggtt tcttgggggc
     6721 agctggatct actatgggag ccgcatcaat cactttgaca gtgcaggcca gacaatacct
     6781 gtcaggaatc gttcagcagc aatcaaatct cttgcaagct atcgaggctc aacaacactt
     6841 gttgcagctt acggtttggg ggatcaaaca gcttcaaacc cgggtacttg ctatcgaaag
     6901 atatttgaag gaccaacaac tcctcggtat ctgggggtgc agtggaaaat tgatctgtac
     6961 tactgccgtg ccttggaatt ctagttggtc taacaaaagt ctcacggaca tctgggacaa
     7021 tatgacatgg atgcagtggg atagagaggt gagcaactat accggaatta tttatcgcct
     7081 tctggaggac tctcagaatc aacaagagag gaacgagcaa gacctcctcg cgctcgattc
     7141 ctggaagaat ctttggtcat ggtttgatat ctcaaattgg ctctggtata taaaaatttt
     7201 cataatgata gtaggtgggt tgattggtct tcgcatcatt tttgcggtat tgtctatcgt
     7261 caaccgagta agacaggggt attccccatt gtctttccaa acattgaccc caaacccgac
     7321 tggccccgac agactcggga gaatcgaaga agagggaggt gagcaggata gagataggag
     7381 cgtgaggctt gtgaacggtt tcctggcgct tgcttgggat gacctgcgaa gtctttgtct
     7441 tttttgttat caccgcctga gggatttcat actggtaata gcgcgcgcgg tcgaattgtt
     7501 gggacgatcc tcactgcggg ggctccagcg gggttgggaa gctttgaagt atcttggatc
     7561 cattgttcaa tactgggggc ttgaattgaa gaaaagtgcc atctctttgc tcgatgtgat
     7621 cgcaatagct gttgcggaag gtacagacag aatcattgat ctcgtacttc gaacctgccg
     7681 cgcgatacgc aatataccca gacggctgcg acaggggttt gagactgcgc tgctctagta
     7741 actcgaggcc ggcaaggccg gatccagaca tgataagata cattgatgag tttggacaaa
     7801 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     7861 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     7921 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     7981 gtggtatggc tgattatgat ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     8041 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     8101 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaggtc
     8161 gactctagag gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc
     8221 cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactggg
     8281 ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct gactgtagtt
     8341 gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca tcctggagca
     8401 gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag
     8461 ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc
     8521 tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc
     8581 attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc
     8641 gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg
     8701 aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca
     8761 ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc
     8821 attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc
     8881 ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca
     8941 gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt
     9001 tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc
     9061 ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc
     9121 catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg
     9181 ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg
     9241 gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga
     9301 cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc
     9361 cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag
     9421 tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg
     9481 taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa
     9541 ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg
     9601 cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg
     9661 ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt
     9721 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     9781 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     9841 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
     9901 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
     9961 tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca
    10021 tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg
    10081 ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
    10141 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg
    10201 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
    10261 ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gcatatacag tcagcatatg
    10321 atacccagta gtagagtggg agtgctatcc tttgcatatg ccgccacctc ccaagggggc
    10381 gtgaattttc gctgcttgtc cttttcctgc tggttgctcc cattcttagg tgaatttaag
    10441 gaggccaggc taaagccgtc gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt
    10501 tccaacgcga gaaggtgttg agcgcggagc tgagtgacgt gacaacatgg gtatgcccaa
    10561 ttgccccatg ttgggaggac gaaaatggtg acaagacaga tggccagaaa tacaccaaca
    10621 gcacgcatga tgtctactgg ggatttattc tttagtgcgg gggaatacac ggcttttaat
    10681 acgattgagg gcgtctccta acaagttaca tcactcctgc ccttcctcac cctcatctcc
    10741 atcacctcct tcatctccgt catctccgtc atcaccctcc gcggcagccc cttccaccat
    10801 aggtggaaac cagggaggca aatctactcc atcgtcaaag ctgcacacag tcaccctgat
    10861 attgcaggta ggagcgggct ttgtcataac aaggtcctta atcgcatcct tcaaaacctc
    10921 agcaaatata tgagtttgta aaaagaccat gaaataacag acaatggact cccttagcgg
    10981 gccaggttgt gggccgggtc caggggccat tccaaagggg agacgactca atggtgtaag
    11041 acgacattgt ggaatagca
//
