LOCUS       sequence-240451-        2811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Alpha 1 destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: Alpha1
FEATURES             Location/Qualifiers
     source          1..2811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        42..89
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        143..161
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     143..160
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    168..224
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(226..245)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(227..245)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(252..269)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(252..268)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(261..283)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(477..496)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(632..1220)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(712..731)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1335..1437
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1438..2253
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1438..2253
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1511..1530)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2367..2795
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2454..2473)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2664..2685
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcttacaatt ttaacgaatt cgtctcagga gagagacccc tgttgacaat taatcatcgg
       61 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaacccag gaggcagatc
      121 atgaccatga ttacgccaag ctatttaggt gacactatag aatactcaag cttgcatgcc
      181 tgcaggtcga ctctagagga tccccgggta ccgagctcga attcgcccta tagtgagtcg
      241 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      301 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
      361 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgacg cctgatgcgg
      421 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
      481 atctgctctg atgccgcata gggtctctcg ctgtcatgag acgaattctg acacggttat
      541 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      601 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      661 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      721 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      781 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      841 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      901 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      961 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1021 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1081 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1141 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1201 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1261 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1321 agatcctttt gtgatgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga
     1381 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg
     1441 agccatattc aacgggaaac gtcttgctca aggccgcgat taaattccaa catggatgct
     1501 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctac
     1561 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     1621 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     1681 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     1741 ccagggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     1801 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     1861 aacagcgatc gcgtatttcg actcgctcag gcgcaatcac gaatgaataa cggtttggtt
     1921 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     1981 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     2041 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     2101 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     2161 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     2221 cagtttcatt tgatgctcga tgagtttttc tgattttttt taaggcagtt attggtgccc
     2281 ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc
     2341 gatgataagc tgtcaaacat gatgttacgc gccctgtagc ggcgcattaa gcgcggcggg
     2401 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     2461 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2521 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2581 ttagggtgat ggttcacgta gtgggccagc gccctgatag acggtttttc gccctttgac
     2641 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     2701 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     2761 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa c
//
