LOCUS       sequence-240454-       10662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; 
            C-terminal truncation; QES mutant for enhanced presentation of 
            quaternary epitopes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 10662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-CH111-QES.c13.L544Y-754*
FEATURES             Location/Qualifiers
     source          1..10662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        56..160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(161..229,230..1021)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRXDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPXAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(379..398)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1192..1780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1681..1700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2211..2356
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2399..3436
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3434..3453)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3478..3526
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4180..4559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4560..4763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4714..4734
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4760..4784
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7084..7218
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7108..7127)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7162..7181
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7361..7383)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7943..9732
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 antcrkgaka wywmysgwty cktmgacgtc aggtggcact tttcggggaa atgtgcgcgg
       61 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      121 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      181 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      241 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
      301 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
      361 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtrttgacg ccgggcaaga
      421 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
      481 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
      541 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
      601 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
      661 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgyagcaa tggcaacaac
      721 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
      781 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
      841 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
      901 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
      961 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     1021 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     1081 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1141 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1201 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1261 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1321 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1381 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1441 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1501 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1561 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1621 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1681 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1741 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1801 tttacggttc ctggcctttt gctggccttg aagctgtccc tgatggtcgt catctacctg
     1861 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     1921 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     1981 ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc
     2041 caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga
     2101 gtggtgaatc cgttagcgag gtgccgccct gcttcatccc cgtggcccgt tgctcgcgtt
     2161 tgctggcggt gtccccggaa gaaatatatt tgcatgtctt tagttctatg atgacacaaa
     2221 ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg
     2281 gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct
     2341 gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat cccggggggc aatgagatat
     2401 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     2461 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     2521 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     2581 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     2641 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     2701 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     2761 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     2821 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     2881 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     2941 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     3001 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     3061 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     3121 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     3181 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     3241 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     3301 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     3361 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccgg atcgggagat
     3421 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     3481 caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt
     3541 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     3601 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     3661 gccaacgtcg gggcggcagg ccctgccata gccactggcc ccgtgggtta gggacggggt
     3721 cccccatggg gaatggttta tggttcgtgg gggttattat tttgggcgtt gcgtggggtc
     3781 aggtccacga ctggactgag cagacagacc catggttttt ggatggcctg ggcatggacc
     3841 gcatgtactg gcgcgacacg aacaccgggc gtctgtggct gccaaacacc cccgaccccc
     3901 aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg accaattctc atgtttgaca
     3961 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg
     4021 tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc attggttata
     4081 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
     4141 acatttatat tggctcatgt ccaatatgac cgccatgttg acattgatta ttgactagtt
     4201 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     4261 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     4321 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     4381 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc
     4441 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     4501 ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     4561 tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc
     4621 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     4681 ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt
     4741 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatctctaga agctgggtac
     4801 cagctgctag cgccaccatg cccatgggta gtctgcagcc tttggctact ctttacttgt
     4861 tgggtatgtt ggtagcgagc gtcctcgcag tcgggaattt gtgggttaca gtttattacg
     4921 gggtcccggt gtggaaggag gcgacgacca cattgttttg cgcctccgat gcaaaggctt
     4981 acgacacgga agttcacaac gtttgggcca cccatgcgtg cgtgcctacg gaccctaatc
     5041 cacaggagat gttcatgccc aatgtcaccg aaaattttaa tatgtggaaa aacgacatgg
     5101 taaatcaaat gcatgaggat gtcataagtc tctgggacca aagtctgaag ccatgcgtga
     5161 aattgacccc gctctgcgtg acactggagt gtaaaaacgt gtcacgaaat aattccagtt
     5221 caaatggtac tggtacaaac aatgaagcgt atcgagaggg aatgaaggaa ataaagaact
     5281 gctcttttaa tgccactgcc gagcttagag accggaagca gaacgtgtat gcactgtttt
     5341 acaggcttga catcgtacct cttgcggaga acagtagcga atataggctt atcaattgca
     5401 acaccagcgc aattactcaa gcctgcccca aaatgacttt tgacccgatt cctatacact
     5461 actgcacacc agcagggtac gcaattctta agtgtagcga caaaaccttc aacggcactg
     5521 gcccctgttc aaatgtctcc acagttcaat gtactcacgg aatacgaccg gtcatgagta
     5581 cacagttgct gctgaacggt tctctggccg aagaggagat catcatccgc tctgagaacc
     5641 tcacgaacaa tgccaagcca ataatcgtac agcttaacca gagcgtggaa atagtttgca
     5701 cacgccctaa caataacact agaaagtcaa tcaggatcgg accagggcag acgttttatg
     5761 cgacaggagg aataataggc gacatccgac aagcgtactg taacatttcc cggaataaat
     5821 ggaacgagac attgagaatg gtaggtaaaa aattggcgga acattttcct aacaaaacca
     5881 tcaattttac atcctcctcc gggggagatc tggaaatcac gacgcacagt tttacgtgta
     5941 ggggagaatt cttctattgc aacacctccg gactgtttaa tggtacgtac atgcccaacg
     6001 gtacgtatat gttcaactct acgaatgcca cgatcacaat cccgtgtaga attaagcaga
     6061 tcatcaatat gtggcaaggg gtcggccgag ccatatatgc tccgcctata gcgggcaata
     6121 tcacctgcaa atccaacata acgggtcttc ttcttgtacg cgacggtgga gaaacaaacg
     6181 ataccaatac caccgagaca tttagaccag gcggaggaga tatgcgagat aattggcgga
     6241 gcgaattgta caagtacaag gtggtcgaga taaagccttt gggtgtggca cccacgggcg
     6301 caaagcgcag aatagtcgga agagaaaaga gagcagttgg gctcggcgct gcttttcttg
     6361 gtttcctcag cgttgccggt agtacgatgg gagcagcgtc aatgaccctg actgtccaag
     6421 cgcgacagta ccttagcgga atagtgcaac aacagagcaa cctgctgcgg gcaattgaag
     6481 cacagcagca tttgctccaa ctgactgtct ggggtataaa gcaactgcag gccagggttt
     6541 tggccataga gagatacctc aaagatcaac agctcctggg tatctggggg tgttcaggaa
     6601 agctcatctg tacgaccaca gtaccctgga actcttcctg gagcaataaa aaccttactg
     6661 agatctggga taatatgact tggatgcagt gggacaagga aatctccaac tacaccaata
     6721 cgatttattg gttgcttgag gagtcacaga atcagcaaga gaagaacgaa aaggatctcc
     6781 tcgccctcga ttcatggaac aatttgtgga actggttcga tatttctaaa tggctgtggt
     6841 acatcaagat attcataatg atcgtcggcg gacttatcgg tctccgaatc atatttgcgg
     6901 tactcagtat cgtgaacagg gtacggcaag ggtacagtcc ccttagcttt caaacactta
     6961 ccccgaaccc tggcggacct gacaggcttg gacgaatcga ggaggaagga ggagaacaag
     7021 atcgccgcag gtctattaga ctggtatccg gcttcctctg ataactcgag gccggcaagg
     7081 ccggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     7141 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     7201 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     7261 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     7321 gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     7381 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     7441 cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgactcta gaggatcgat
     7501 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     7561 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     7621 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     7681 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     7741 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     7801 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     7861 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     7921 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     7981 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     8041 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     8101 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     8161 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     8221 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     8281 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     8341 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     8401 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     8461 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     8521 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     8581 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     8641 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     8701 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     8761 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     8821 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     8881 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     8941 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     9001 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     9061 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     9121 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     9181 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     9241 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     9301 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     9361 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     9421 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     9481 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     9541 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     9601 cgggtagcat atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt
     9661 gggagtgcta tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt
     9721 gtccttttcc tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc
     9781 gtcgcatgtc tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg
     9841 ttgagcgcgg agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag
     9901 gacgaaaatg gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac
     9961 tggggattta ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc
    10021 ctaacaagtt acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc
    10081 cgtcatctcc gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag
    10141 gcaaatctac tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg
    10201 gctttgtcat aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt
    10261 gtaaaaagac catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg
    10321 gtccaggggc cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag
    10381 caagggcagt tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca
    10441 caccggcgac ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag
    10501 ctcttaaacc ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct
    10561 ttccaaacca ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc
    10621 ttgggccttc tcctgggtca tctgcggggc cctgctctat cg
//
