LOCUS       sequence-240461-        2653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Omega 2 destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: Omega 2
FEATURES             Location/Qualifiers
     source          1..2653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        23..70
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        124..142
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     124..141
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    149..205
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(207..226)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(208..226)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(233..250)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(233..249)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(242..264)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(458..477)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(609..1197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(689..708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1312..1414
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1415..2074
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1481..1500)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2187..2642
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2274..2293)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2484..2505
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctcagtcagg agagagacgc ctgttgacaa ttaatcatcg gcatagtata tcggcatagt
       61 ataatacgac aaggtgagga actaaaccca ggaggcagat catgaccatg attacgccaa
      121 gctatttagg tgacactata gaatactcaa gcttgcatgc ctgcaggtcg actctagagg
      181 atccccgggt accgagctcg aattcgccct atagtgagtc gtattacaat tcactggccg
      241 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
      301 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
      361 aacagttgcg cagcctgaat ggcgaatgac gcctgatgcg gtattttctc cttacgcatc
      421 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
      481 agcgtctctc gcttgagacc tgcagtgaca cggttatcca cagaatcagg ggataacgca
      541 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      601 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      661 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      721 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      781 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      841 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      901 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      961 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1021 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1081 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1141 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1201 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1261 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttgtg atgatcggca
     1321 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga
     1381 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1441 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1501 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1561 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1621 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     1681 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     1741 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1801 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtgt cagccaatcc
     1861 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     1921 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     1981 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     2041 acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc
     2101 ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc
     2161 gatgataagc tgtcaaacat gatgttacgc gccctgtagc ggcgcattaa gcgcggcggg
     2221 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     2281 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2341 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2401 ttagggtgat ggttcacgta gtgggccagc gccctgatag acggtttttc gccctttgac
     2461 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     2521 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     2581 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     2641 tttaactgca ggt
//
