LOCUS       sequence-240465-       16066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses myc tagged mouse reelin ORF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16066)
  AUTHORS   D'Arcangelo G, Nakajima K, Miyata T, Ogawa M, Mikoshiba K, Curran T
  TITLE     Reelin is a secreted glycoprotein recognized by the CR-50 monoclonal
            antibody.
  JOURNAL   J Neurosci. 1997 Jan 1;17(1):23-31.
  PUBMED    8987733
REFERENCE   2  (bases 1 to 16066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "1997-01-1"; volume: "17(1)"; pages: "23-31"
COMMENT     Sequence Label: pCrlM
FEATURES             Location/Qualifiers
     source          1..16066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2545..2556
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(4597..4608)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(11568..11584)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11568..11584)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(11619..11637)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11620..11637)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11657..11674)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11663..11887
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11933..12361
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12020..12039)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12230..12251
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12370..12390)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12375..12704
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12555..12690
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12617..12636
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             12771..13565
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12825..12844)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13435..13454
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    13739..13860
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13776..13795)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13830..13849
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13909..13925)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13909..13925)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13922..13944)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13933..13949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13957..13963,13964..13981,13982..13987))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14002..14023
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14140..14157)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14311..14899)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14391..14410)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15070..15861,15862..15930))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15693..15712
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15931..16035)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagag gctcgccttc
      961 cccgcacgcg gctcctccgt gccggtgcct ccgaaagtgg atgagagagc gcgcggggcg
     1021 cgcggcggca cggagcgcgg cggcatggag cgcggctgct gggcgccgcg ggctctcgtc
     1081 ctggccgtgc tgctgctgct ggcgacgctg agggcgcgcg cggccaccgg ctactacccg
     1141 cgcttctcgc ctttcttttt cctgtgcacc caccacgggg agctggaagg ggatggggag
     1201 cagggcgagg tgctcatttc cctgcacatt gcgggcaacc ccacctacta cgtaccggga
     1261 caggaatacc atgttacaat ttcaacaagc accttctttg atggcttgct ggtgacggga
     1321 ctctatacct cgacaagcat ccagtcttct cagagcattg gaggctccag cgcctttgga
     1381 ttcgggatca tgtccgacca ccagtttggt aaccagttta tgtgcagtgt ggtggcctct
     1441 catgtgagtc acctgcctac aaccaacctc agctttgtct ggattgcccc accagctggc
     1501 acaggctgtg tgaatttcat ggctactgca acacataggg gccaggtgat tttcaaagac
     1561 gcactggccc agcagctgtg tgaacaagga gctcccacag aggccactgc ttactcgcac
     1621 cttgctgaaa tacacagtga cagtgtgatc ctacgagatg actttgactc ctaccagcaa
     1681 ctggaattga accccaacat atgggttgaa tgcagcaact gtgagatggg agagcagtgt
     1741 ggcaccatca tgcatggcaa tgctgtcacc ttctgtgagc cgtacggccc tcgagagctg
     1801 accaccacat gcctgaacac aacaacagca tctgtcctcc agttttccat tgggtcagga
     1861 tcatgtcgat ttagttactc tgaccccagc atcactgtgt catacgccaa gaacaatacc
     1921 gctgattgga ttcagctgga gaaaattaga gccccttcca atgtgagcac agtcatccac
     1981 atcctgtacc tccccgagga agccaaaggg gagagcgtgc agttccagtg gaaacaggac
     2041 agcctgcgag tgggtgaggt gtatgaggcc tgctgggccc tggataacat cctggtcatc
     2101 aattcagccc acagagaagt cgttctggag gacaacctcg acccggtcga cacgggcaac
     2161 tggctcttct tccctggagc aacggtcaag catagctgtc agtcagatgg gaactccatt
     2221 tatttccatg gaaatgaagg cagcgagttc aattttgcca ccacccggga tgtagatctt
     2281 tctacagagg atattcaaga gcagtggtca gaagaatttg agagccagcc cacaggatgg
     2341 gatatcttgg gagcagtagt tggtgcagac tgtggaaccg tagaatcagg actatcactg
     2401 gtgttcctca aagatggaga gaggaagctt tgcaccccct acatggatac aactggttat
     2461 ggcaacctga ggttctactt cgttatggga ggaatctgtg accctggagt ctctcatgaa
     2521 aacgatatca tcttatatgc aaagattgaa ggaagaaaag aacacattgc actggacact
     2581 cttacctatt cttcctataa ggttccgtct ttggtttctg tggtcatcaa ccctgaactt
     2641 cagacacctg ccaccaaatt ttgtctcagg caaaagagcc accaagggta taatcggaat
     2701 gtctgggctg tggacttctt ccatgtgctg cccgttctcc cttcaacaat gtctcacatg
     2761 atccagtttt ctattaattt gggatgcggc acacaccagc ctgggaacag cgtcagcttg
     2821 gagttttcta ctaaccatgg acggtcctgg tccctactcc acactgagtg cttgccggag
     2881 atctgtgcag gcccccacct cccccacagc actgtctact cctcagaaaa ctacagcggg
     2941 tggaaccgaa tcacgattcc tctccctaat gcagcactca cccgagacac caggattcgc
     3001 tggagacaaa caggcccaat cctgggaaat atgtgggcaa ttgataatgt ttatataggt
     3061 ccttcgtgtc tcaaattctg ttctggcaga ggacaatgca ctcggcatgg ctgcaagtgt
     3121 gacccaggat tttctggccc agcttgtgag atggcatctc agacattccc aatgtttatt
     3181 tcggaaagct ttggcagtgc cagactttcc tcttaccata acttttactc tatccgtggt
     3241 gctgaagtca gctttggttg tggtgtctta gccagtggta aggctctggt tttcaacaaa
     3301 gatgggaggc ggcagctaat cacgtccttt ctggacagct cgcagtccag gtttcttcag
     3361 tttacactga ggctggggag caagtctgtg ctgagcacgt gcagagcccc tgaccagccg
     3421 ggggagggag tcctgctgca ctattcatat gacaacggga taacatggaa actcctggag
     3481 cactattcct acgtcaacta ccacgagccc agaataatct ctgtagagct accggatgat
     3541 gcaagacagt ttggaatcca gttcagatgg tggcagcctt accattcttc ccaaggagaa
     3601 gacgtgtggg ccattgatga gattgtcatg acctcagtcc tgttcaacag catcagtctc
     3661 gactttacca atcttgtgga agtcactcaa tccctgggat tctaccttgg caatgttcaa
     3721 ccatactgtg gccatgactg gacgctttgt tttacgggag attctaaact tgcctcaagc
     3781 atgcgctatg tggaaacaca gtccatgcag atcggagcat cctatatgat tcagttcagc
     3841 ctagtgatgg gatgtggcca gaaatacact cctcacatgg acaaccaggt gaagctggag
     3901 tactcagcca accacggcct tacatggcac cttgtacaag aagaatgcct tcccagtatg
     3961 ccaagctgcc aggaatttac atctgccagc atttaccatg ccagcgagtt cacacagtgg
     4021 agaagagtca ctgttgttct tccccagaaa acatggtccg gtgccacccg cttccgttgg
     4081 agtcagagct attacacagc ccaggatgag tgggctttag acaacattta cattgggcag
     4141 cagtgcccca acatgtgcag tgggcatggc tcatgtgacc atggcgtgtg caggtgtgac
     4201 cagggatacc agggcactga atgccaccca gaagctgcac ttccttccac gattatgtca
     4261 gattttgaga acccgagcag ttgggaatca gactggcagg aagttattgg gggagaagtt
     4321 gtaaagcctg agcaaggctg tggagtcgtg tcttctggat cttctctgta cttcagcaag
     4381 gctgggaaga ggcagctggt gagctgggac ctggacacat cctgggtgga ctttgtccag
     4441 ttctacatcc agataggagg agagagtgct gcatgcaaca agcctgacag cagagaggag
     4501 ggcattctgc tccagtatag caacaacggg ggcatccagt ggcacctgct ggcagagatg
     4561 tacttctcag acttcagcaa acccagattt gtctacctgg agctcccagc tgctgcgaag
     4621 accccttgta ccaggttccg ctggtggcag cctgtgttct cgggggagga ctatgaccag
     4681 tgggccgttg atgatatcat cattctgtca gagaagcaga agcaggttat tccagttgtc
     4741 aacccaactt tgccccagaa cttctatgag aagccagctt tcgattaccc tatgaaccaa
     4801 atgagtgtgt ggctaatgtt ggccaatgaa ggcatggcta aaaacgacag cttctgtgcg
     4861 accacgccgt cagccatggt gtttggaaag tcagatggag accggtttgc agtaactcga
     4921 gatctgaccc tgaaacctgg atatgtgctg cagttcaagc taaacatagg ctgcaccagc
     4981 cagttcagca gcactgcccc ggttctcctg cagtattcac atgatgccgg catgtcgtgg
     5041 tttctggtga aggaaggatg cttcccagcg tcagcaggca aaggatgtga agggaactcc
     5101 agggaattga gtgagcctac tgtctattat actggggact tcgaagaatg gactagaatc
     5161 accattgcca ttccaaggtc ccttgcatcc agcaagacca gattccgatg gatccaagag
     5221 agcagctctc agaagaatgt gcccccgttt ggcttagatg gggtgtacat atctgagcct
     5281 tgtcccagtt actgcagtgg ccatggagac tgcatctcgg gggtgtgttt ttgtgacctg
     5341 gggtacacag ctgcacaagg aacctgtgtg tcaaacaccc ctaaccacag tgagatgttc
     5401 gacaggtttg aggggaagct aagcccactg tggtacaaaa tcaccggggg tcaggttggc
     5461 acgggctgtg gcaccctcaa tgacggcagg tccctctact ttaatggcct tgggaaaagg
     5521 gaagccagga cagtcccact ggacaccagg aatatcagac ttgttcagtt ttatatacaa
     5581 attggaagta aaacatcagg gattacgtgc atcaaaccac gggctagaaa tgaggggctt
     5641 gttgttcagt attccaatga taatgggata ctttggcatt tgctgagaga gttggatttc
     5701 atgtcattcc tggagccaca gatcatttcc attgacctgc cccgggaagc aaagacacct
     5761 gccacagctt tccggtggtg gcagccgcag catgggaagc attcggccca gtgggctttg
     5821 gatgatgtcc ttataggagt gaatgacagc tctcaaactg gatttcaaga taaatttgat
     5881 ggctccatag acttgcaagc caactggtat cgaatccagg gaggccaagt tgatatcgac
     5941 tgcctctcta tggacactgc ccttatattc actgaaaaca taggaaaacc tcgctatgct
     6001 gagacctggg acttccatgt gtcagcgtca agcttcttac agtttgaaat gaacatgggc
     6061 tgcagcaagc ctttcagtgg tgcccacggc atacagctcc agtactctct gaacaacggc
     6121 aaggactggc agcttgtcac cgaagagtgt gtccctccaa ccattggctg cgtgcactac
     6181 acagagagtt caacttacac atcagaaaga ttccagaact ggaggcgggt cacggtctac
     6241 ctgccactcg ccaccaattc tcccaggact cggttcagat ggattcagac caactatact
     6301 gttggagcag attcctgggc tattgataat gtcatcctgg cctcgggctg tccttggatg
     6361 tgctcaggac gagggatctg tgattcgggg cgctgtgtgt gtgaccgggg cttcggtgga
     6421 cccttctgtg ttcctgttgt tcctcttccc tccattctaa aagatgattt caatgggaac
     6481 ttacatcctg acctttggcc tgaagtgtac ggggcagaga ggggcaatct gaatggcgaa
     6541 accatcaaat ccggacagaa gctgatatcc gaggaggacc tgaactccgg aacatgtctg
     6601 atctttaaag gggagggact aagaatgctt atttccagag atctagattg taccaatact
     6661 atgtatgtcc agttctctct ccgatttata gcgaaaggta ccccagagag gtctcactcc
     6721 atccttctac agttctctgt cagtggagga gtcacctggc acctgatgga tgaattctac
     6781 ttccctcaaa cgaccagcat acttttcatc aatgttccct taccatacgg tgcccaaacc
     6841 aacgctacaa gattcagact ctggcaaccg tacaataatg gtaagaaaga agaaatctgg
     6901 atcattgatg actttattat tgatggaaac aatttgaaca accccgtgct gctgctggac
     6961 acgttcgact ttgggcccag ggaagacaat tggtttttct atccgggtgg taatatcgga
     7021 ctttactgcc cgtattcttc aaagggagct cctgaggagg attcggccat ggtgtttgtt
     7081 tcaaacgaag ttggagaaca ctccattacc acacgagacc taagtgtgaa cgagaacacc
     7141 atcattcaat ttgagatcaa tgttggctgc tccactgata gttcttctgc tgatccggtc
     7201 agactggaat tctcaaggga ctttggagcc acctggcacc tgctgctgcc tctctgctac
     7261 cacagcagca gcctcgtcag ctccttatgc tccactgagc atcacccgag cagcacctac
     7321 tacgcgggga ccacccaggg ctggcggcgg gaggtcgtgc acttcggaaa gctgcacctt
     7381 tgtggatctg tgcgtttccg ttggtaccag ggattttatc ctgctggctc tcagccggtc
     7441 acatgggcca ttgacaatgt ctacattggt ccccagtgtg aagagatgtg ctatgggcac
     7501 gggagctgca tcaatggaac caagtgtata tgtgacccgg gctactctgg gccaacctgt
     7561 aaaataagca ccaaaaatcc tgattttctc aaagacgact ttgaaggtca actggaatcc
     7621 gatcgattct tactgatgag cggtgggaag ccgtctcgta agtgtggcat cctttccagt
     7681 gggaacaacc tcttcttcaa tgaggacggc ttgcgcatgc tagtaacacg ggacctggat
     7741 ttatcacatg caaggtttgt gcagttcttc atgagactgg gatgtggtaa aggtgttcca
     7801 gaccccagga gccagcccgt gcttctgcag tactccctca atggcggcct ctcctggagt
     7861 cttcttcaag agttcctctt cagcaactcc agcaatgtgg gcaggtacat tgccctggaa
     7921 atgcccctga aagcccgttc tggttcgaca cgcctccgct ggtggcagcc atctgaaaat
     7981 gggcacttct atagcccctg ggtgatcgac cagattctta ttggaggaaa tatctctggt
     8041 aatacagtct tagaagatga tttctcaact ctggacagca gaaagtggct gcttcaccca
     8101 ggaggcacca agatgcctgt gtgtggctcc acaggcgatg ccctggtctt tattgaaaag
     8161 gccagcaccc gttacgtggt cacgacagac atcgctgtga atgaggactc attcctacag
     8221 atagactttg ctgcctcctg ctcagtcaca gactcctgct atgctattga actggagtac
     8281 tcggtggatc tcggtctgtc gtggcacccg ctggtgaggg actgcctgcc taccaatgtt
     8341 gagtgtagtc gttaccacct gcagcggatc ctggtgtcag atactttcaa caagtggacc
     8401 agaatcactc tgcccctgcc ttcctacacc aggtctcaag ccactcgttt ccgctggcat
     8461 cagccagcgc cttttgacaa gcagcagacc tgggcaatag ataatgtcta tattggggat
     8521 ggttgcctag acatgtgcag tggccacggg agatgcgtcc agggaagctg tgtctgtgat
     8581 gaacagtggg gaggcctgta ctgtgatgag cctgagacct cccttcccac ccagctcaaa
     8641 gacaacttca accgagcccc ctccaaccag aactggctga ctgtgagcgg tgggaagctg
     8701 agtacagtgt gtggggctgt ggcttccggc ctggctctcc atttcagtgg gggctgcagc
     8761 cgattgttag tcactgtgga tctgaacctc accaatgctg agtttatcca gttttacttt
     8821 atgtatggat gcctcattac gccgagcaac cgtaaccagg gagtcctgct ggagtactct
     8881 gtcaatggag gcatcacctg gaacttgctg atggagattt tctatgacca gtacagcaaa
     8941 cctggatttg tgaatatcct tctccctcct gatgctaaag agattgccac tcgcttccga
     9001 tggtggcagc cacgacatga tggccttgac cagaatgact gggccattga caatgtcctc
     9061 atctcgggct ctgcggacca gaggacagtc atgctggaca cctttagcag cgccccagta
     9121 ccacagcatg agcgctcccc cgcagacgct ggccctgttg gaagaattgc ttttgaaatg
     9181 ttcttagaag acaaaacttc agtgaatgag aattggctct tccatgatga ctgtacagtg
     9241 gaaagattct gtgactcgcc agatggtgtc atgctctgtg gcagccatga tggacgagag
     9301 gtgtatgcag tgactcatga cctgacgccc actgagaact ggatcatgca gttcaagatc
     9361 tctgttggat gcaaagtgcc tgaaaaaatt gcccagaatc aaattcacgt gcagttttct
     9421 actgactttg gcgtgagctg gagttattta gtccctcagt gcttacccgc cgacccaaag
     9481 tgttctggaa gcgtttctca accgtctgtg ttcttcccaa ctgaagggtg gaaaaggatc
     9541 acctacccgc ttcctgaaag cttaacgggg aatcctgtaa gatttaggtt ctaccaaaag
     9601 tactcagatg tgcagtgggc aattgacaat ttctaccttg gccctggatg tttggacaac
     9661 tgtggaggcc acggagactg cctaaaggaa cagtgtatct gtgacccagg ctactcaggg
     9721 ccaaactgct acttaactca cagcctgaag actttcctga aggagcgctt tgacagtgag
     9781 gagatcaagc ctgacttatg gatgtccttg gaaggcggaa gcacttgtac agagtgcggg
     9841 gtcctcgccg agaacactgc actctatttt gggggatcca ctgtgagaca agctattact
     9901 caagacttag atctcagagg tgcaaaattc ctgcagtact ggggacgtat cggcagtgag
     9961 aacaacatga catcttgcca tcggcctgtc tgccggaagg aaggcgtgct gctggacttc
    10021 tctacggatg gaggaatcac ttggaccttg cttcacgaga tggatttcca gaaatacatt
    10081 tctgtgaggc acgactacat cctcctgcct gagggggccc tcaccaacac aactcgactt
    10141 cgctggtggc agccttttgt catcagcaat gggctcgtgg tttccggggt ggagcgtgcg
    10201 cagtgggcac tggacaacat tctgattggt ggagcagaaa tcaatccaag ccaactggtg
    10261 gacactttcg atgacgaagg ctcctcccat gaagaaaact ggagttttta ccctaatgca
    10321 gtaaggacag caggattctg tggcaaccca tccttccacc tctactggcc aaataaaaag
    10381 aaggacaaga cccacaatgc actctcctcc cgagagctca ttatacagcc aggatacatg
    10441 atgcaattta aaattgtggt gggttgtgaa gccacttcat gtggtgacct tcattccgtg
    10501 atgctggagt acaccaagga tgcaaggtcc gattcctggc agctcgtgca gacccagtgc
    10561 ctaccttcct cttccaatag cattggctgc tccccgttcc agttccatga agccaccatt
    10621 tataatgctg tcaacagctc aagctggaag aggatcacca tccagctccc agaccacgtc
    10681 tcgtcaagtg ccacacagtt ccgctggatc cagaagggag aagaaaccga gaagcaaagc
    10741 tgggccatcg accacgtgta catcggagag gcttgtccca agctctgcag cgggcatggc
    10801 tactgcacca caggggccgt ctgcatctgc gatgaaagct tccaaggtga cgactgctct
    10861 gtcttcagtc acgagcttcc tagttacatt aaagataatt ttgaatcagc aagagtcact
    10921 gaagccaact gggaaaccat ccagggtgga gtgatcggaa gtggctgtgg gcagctggcg
    10981 ccctatgccc atggagattc gctctacttt aatggttgtc agataaggca agctgccacc
    11041 aagccactgg acctcactcg agcaagcaaa attatgtttg tcttgcaaat tgggagccca
    11101 gcccagacag acagttgcaa cagcgacctc agcggccccc acaccgtgga caaagcagta
    11161 ctgctgcagt acagtgtcaa caatggcatc acctggcacg tcatcgctca gcaccagccg
    11221 aaggacttca cacaagctca gcgggtgtct tacaacgtcc ccctggaagc tcggatgaaa
    11281 ggagttctac tgcgctggtg gcagccacgc cacaatggaa caggtcatga tcaatgggct
    11341 ttggaccatg tggaggtcgt cctagtaagc actcgcaaac aaaattacat gatgaatttt
    11401 tcacggcaac atgggctcag gcacttctac aacagaagac gaaggtcgct taggcgatac
    11461 ccatgaagaa tccaagttta tttccctttc cagcgtacaa tgtgtccctt cctggttttt
    11521 tgaaacacct ctcactgcat ctgatatcga attcctgcag cccgggggat ccactagttc
    11581 tagagcggcc gctcgagcat gcatctagag ggccctattc tatagtgtca cctaaatgct
    11641 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
    11701 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
    11761 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
    11821 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
    11881 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
    11941 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
    12001 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
    12061 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
    12121 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
    12181 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    12241 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
    12301 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
    12361 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
    12421 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
    12481 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    12541 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    12601 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
    12661 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
    12721 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
    12781 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
    12841 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
    12901 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
    12961 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
    13021 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
    13081 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
    13141 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
    13201 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
    13261 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
    13321 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
    13381 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
    13441 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
    13501 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
    13561 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
    13621 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
    13681 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
    13741 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    13801 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    13861 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
    13921 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    13981 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    14041 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    14101 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    14161 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    14221 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    14281 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    14341 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    14401 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    14461 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    14521 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    14581 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    14641 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    14701 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    14761 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    14821 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    14881 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    14941 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    15001 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    15061 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    15121 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    15181 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    15241 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    15301 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    15361 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    15421 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    15481 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    15541 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    15601 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    15661 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    15721 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    15781 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    15841 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    15901 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    15961 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    16021 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
