LOCUS       sequence-240466-        2860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - NLuc Luciferase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pNLuc
FEATURES             Location/Qualifiers
     source          1..2860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..53
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     37..53
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        69..87
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     69..86
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(687..706)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(688..706)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(713..730)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(713..729)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(749..1204)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(886..907)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1098..1117
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(1317..1976)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1891..1910
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1977..2079)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      2194..2782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2683..2702
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgattctg tggataaccg tgtcatgatg cctggacagg aaacagctat gaccaacggc
       61 tctatggtat ttaggtgaca ctatagaacg aattcactag tgattgggcg atgagtggtc
      121 tcgaatggtg tttaccttgg aggacttcgt gggtgactgg aggcaaactg caggatacaa
      181 tttggaccag gtcctcgaac agggaggagt cagcagcctt ttccaaaact tgggcgtgtc
      241 cgttacccct attcagagaa ttgtcctttc cggagagaac ggattgaaaa tagatatcca
      301 cgtcataatc ccgtatgaag gactgtctgg ggaccaaatg ggtcagattg aaaaaatatt
      361 caaagtagtt tatcccgttg atgatcatca cttcaaagtt atccttcatt atgggacttt
      421 ggtaatcgat ggagttactc ctaatatgat agattacttt ggccgacctt acgaggggat
      481 cgctgtattc gacggcaaga aaatcaccgt cactggaacc ctctggaacg gcaacaagat
      541 tattgacgag cgattgatca atccagacgg atctttgctt ttccgcgtaa cgataaacgg
      601 cgtaacaggg tggaggctct gcgaacgaat actcgcctga gcttcgagac cttgagatca
      661 atatctaagg tcctaagcgg aattcaccct atagtgagtc gtattacaat tcactggccg
      721 tcgttttaca tgcgtttcta caatgttaaa attgtaagcg ttaatatttt gttaaaattc
      781 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
      841 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
      901 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
      961 gctggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     1021 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     1081 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     1141 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     1201 gcgtaacatc atgtttgaca gcttatcatc gaatttctgc cattcatccg cttattatca
     1261 cttattcagg cgtagcaacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac
     1321 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
     1381 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     1441 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
     1501 tttaaatcaa aactggtgaa actcacccag ggattggctg acacgaaaaa catattctca
     1561 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     1621 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     1681 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     1741 tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     1801 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     1861 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     1921 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     1981 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     2041 tcattatggt gaaagttgga acctcttacg tgccgatcat cacaaaagga tctaggtgaa
     2101 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2161 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2221 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2281 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2341 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2401 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2461 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2521 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2581 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2641 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2701 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2761 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2821 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
//
