LOCUS       sequence-240468-        3280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - Renilla Luciferase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pRenilla
FEATURES             Location/Qualifiers
     source          1..3280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     173..189
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     173..189
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        205..223
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     205..222
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1243..1262)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1244..1262)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1269..1286)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1269..1285)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1305..1760)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1442..1463)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1654..1673
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(1873..2532)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2447..2466
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2533..2635)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      join(2750..3280,1..58)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3239..3258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
       61 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      121 ctttcctgcg ttatcccctg attctgtgga taaccgtgtc atgatgcctg gacaggaaac
      181 agctatgacc aacggctcta tggtatttag gtgacactat agaacgaatt cactagtgat
      241 tgggcgatga gtggtctcga atgacttcca aggtttatga ccccgagcaa cgcaagagaa
      301 tgataactgg gcctcagtgg tgggccagat gtaaacaaat gaatgtcctt gatagtttca
      361 tcaattacta cgacagtgaa aagcatgcag aaaacgccgt aatttttttg catggcaacg
      421 cagcctcctc ttatttgtgg cgacacgtag tcccgcatat agagccggta gctaggtgta
      481 ttatccctga tctgatcgga atgggaaaga gtggcaagtc agggaatgga tcttatcgcc
      541 ttcttgacca ctacaagtat ctgacggctt ggttcgaact cttgaatttg ccgaagaaaa
      601 tcatcttcgt gggtcacgat tggggagcct gtctcgcgtt ccattattcc tacgagcatc
      661 aggacaagat aaaggcaatc gttcacgccg agtctgtagt agatgtaatc gagtcctggg
      721 acgaatggcc agatattgag gaggatatag cattgattaa aagcgaggaa ggggaaaaga
      781 tggtgctgga gaacaacttc tttgtggaaa caatgcttcc ttcaaaaatc atgagaaaac
      841 tggaacccga ggaatttgcc gcttatctcg aaccgtttaa agaaaaggga gaagtccgca
      901 ggccaacact ttcctggcca agagaaatcc cgctcgtcaa aggcggaaag ccggatgttg
      961 tgcaaatagt ccgaaattac aacgcgtacc tccgggcctc tgacgatctc cctaaaatgt
     1021 ttatagaatc agatccagga ttttttagca acgctatcgt agaaggagca aagaaatttc
     1081 caaataccga atttgtcaaa gtaaagggac tgcatttcag tcaagaggac gctccagacg
     1141 agatgggcaa atacataaag tcctttgtag agagggtcct gaagaacgaa cagtgagctt
     1201 cgagaccttg agatcaatat ctaaggtcct aagcggaatt caccctatag tgagtcgtat
     1261 tacaattcac tggccgtcgt tttacatgcg tttctacaat gttaaaattg taagcgttaa
     1321 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     1381 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     1441 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     1501 aaccgtctat cagggcgctg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     1561 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     1621 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     1681 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     1741 tgcgccgcta cagggcgcgt aacatcatgt ttgacagctt atcatcgaat ttctgccatt
     1801 catccgctta ttatcactta ttcaggcgta gcaaccaggc gtttaagggc accaataact
     1861 gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     1921 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     1981 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     2041 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgacac
     2101 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     2161 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     2221 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     2281 tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg
     2341 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     2401 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     2461 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     2521 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     2581 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcatcaca
     2641 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2701 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2761 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2821 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2881 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2941 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3001 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3061 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3121 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3181 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3241 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
//
