LOCUS       sequence-240471-        3280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - GreenRenilla Luciferase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pGrRenilla
FEATURES             Location/Qualifiers
     source          1..3280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     42..58
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     42..58
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        74..92
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     74..91
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1112..1131)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1113..1131)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1138..1155)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1138..1154)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1174..1629)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1311..1332)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1523..1542
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(1742..2401)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2316..2335
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2402..2504)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      2619..3207
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3108..3127
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatcccctga ttctgtggat aaccgtgtca tgatgcctgg acaggaaaca gctatgacca
       61 acggctctat ggtatttagg tgacactata gaacgaattc actagtgatt gggcgatgag
      121 tggtctcgaa tggcttccaa agtctacgac ccagaacaaa ggaagaggat gattactgga
      181 ccgcagtggt gggcaaggtg caagcagatg aatgtactcg atagttttat caattattac
      241 gactccgaaa aacacgcgga aaatgctgtc atattcctgc acgggaacgc cactagcagc
      301 tacttgtggc ggcacgtagt cccccacata gagcctgttg cccgatgtat tattcccgat
      361 ctcattggaa tgggcaaatc aggcaaatct gggaacggtt cttaccgcct gctcgatcac
      421 tataaatatt tgaccgcttg gtttgagctg cttaatctcc ctaaaaaaat aatattcgtt
      481 ggacacgact ggggctcagc tctggcgttc cactacgcat acgagcatca agaccgaata
      541 aaagcaatag tacatatgga gtcagttgtt gacgtcatag agtcatggat gggttggcca
      601 gatattgaag aggaattggc cctcatcaag agtgaggagg gggaaaaaat ggtcctggaa
      661 aataattttt tcgtggaaac cttgctcccg agtaaaataa tgcgaaaact tgaacccgaa
      721 gagtttgctg cttatttgga gcctttcaag gaaaaggggg aagtaagacg accaaccctt
      781 tcttggccga gggagatacc tcttgttaag ggtggaaagc ccgacgtagt ccagatagtc
      841 cgaaattaca acgcgtacct gagagctagt gatgacctcc ctaagttgtt tatcgagtca
      901 gaccctgggt tcttcagcaa cgcaattgta gaaggagcga aaaaatttcc aaatacagaa
      961 tttgtgaagg ttaaggggct ccactttttg caagaggacg cccccgatga gatgggaaaa
     1021 tacattaaat ctttcgtaga gcgcgtcctt aagaatgaac agtgagcttc gagaccttga
     1081 gatcaatatc taaggtccta agcggaattc accctatagt gagtcgtatt acaattcact
     1141 ggccgtcgtt ttacatgcgt ttctacaatg ttaaaattgt aagcgttaat attttgttaa
     1201 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     1261 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     1321 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     1381 agggcgctgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     1441 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     1501 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     1561 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     1621 agggcgcgta acatcatgtt tgacagctta tcatcgaatt tctgccattc atccgcttat
     1681 tatcacttat tcaggcgtag caaccaggcg tttaagggca ccaataactg ccttaaaaaa
     1741 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     1801 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     1861 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     1921 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgacacg aaaaacatat
     1981 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2041 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2101 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2161 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
     2221 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2281 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2341 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2401 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     2461 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcatcacaa aaggatctag
     2521 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2581 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2641 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2701 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2761 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2821 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2881 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2941 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3001 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3061 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3121 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3181 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3241 gccttttgct ggccttttgc tcacatgttc tttcctgcgt
//
