LOCUS       sequence-240491-        4844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for constitutive expression of ELuc in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: hCMV-IE1:ELuc
FEATURES             Location/Qualifiers
     source          1..4844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        71..374
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        375..578
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     529..549
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2295..2519
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2639..3227)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2719..2738)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3342..3444
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3445..4260
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3445..4260
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3518..3537)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4374..4829
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4461..4480)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4671..4692
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cagtcaggag tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
       61 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      121 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      181 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      241 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      301 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      361 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      421 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      481 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      541 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatca
      601 agctcaaatg atgaagagag agaagaatgt tgtgtatggg cccgagccta agcacccgct
      661 tgggaatttt accgctggag agatgctcta caacgcgctt cacaaacata gtcacatccc
      721 gcaggcgatc ctcgacgtga tgggtaacga aagtcttagt tatcaggaat tcttcgacac
      781 gacggtcaaa ttggggcaga gtctccagaa ttgtggctat aaaatgaatg atgttgtatc
      841 aatatgcgct gaaaacaaca aaagattttt cattccaatt attagtgcgt ggtatatagg
      901 catggtcgtt gcacctgtaa acgaggacta tataccagac gaactgtgta aggtcacagg
      961 aattagtaag ccaatacttg tgttcaccac gcgaaagata cttccaaagg ttttggaggt
     1021 taaggataga acaaattata tcaaaagaat cataattctg gactctgagg aaaaccttct
     1081 cggatgtgaa tccttgcata attttatgtc aagatatagc gataacaatc ttcagacctt
     1141 taaaccactg cattacgatc cggtcgacca ggttgcagcg atactgtgta gtagtggtac
     1201 taccgggttg cctaagggcg tcatgcagac ccaccgcaat atatgtgttc ggctgaccca
     1261 cgcctctgac ccgagagttg gtacccaact cattcccgga gttagtgtgc tggcgtatct
     1321 tcccttcttt cacgcctttg ggttttccat taacttgggg tattttatgg tggggctgcg
     1381 cgtcgtgatg cttcggaggt tcaaccaaga ggtttttttg aaggcgattc aagactatga
     1441 agtacgaagt gtgatcaatg tcccaagtac aatcctcttt ctttctaaat caccgttggt
     1501 tgataagtat gatctttcta ccttggcgga actttgttgt ggtgcggcac ctcttgcaaa
     1561 agaagtagct gaaatcgccg taaaacgcct taatttgcca ggcatacggt gtggttacgg
     1621 gttgactgaa agcacatccg ctaatatcca cactcttcac aacgaattta aatccggttc
     1681 attgggaaaa gtgacgccct acatggccgc taaaattata gatcgaaaca ccggagaggc
     1741 gcttggccca aatcaagtgg gagagttgtg catttggggc cccatggtca cgaaaggcta
     1801 cgtgaataat ccacaggcca ccaaggaggc cattgacgac gatggttggc ttcacagtgg
     1861 tgattttggc tactatgatg aggatgaata tttttacatt gtggatagat ataaagagct
     1921 gatcaagtac aagggatatc aagtggctcc tgtcgagttg gaggagattt tgctccaaca
     1981 tccagggatc cgcgatgtgg ccgtagtcgg tatccccgac atagaagcag gggaacttcc
     2041 ggccggattc gttgtcaagc aacctggggc ccaactgacg gctaaagaag tatatgattt
     2101 ccttgcacaa agagttagcc acagcaagta tcttcgaggg ggtgtcagat tcgttgactc
     2161 tattccgagg aacgtcacgg gcaaaatcag ccgaaaggaa ctccgagaag cacttatgga
     2221 gaaggcgggt ggtggatcta tgtacaagac atccgccgcc aacgatgaga actatgccct
     2281 ggcggcttga gcttctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     2341 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     2401 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     2461 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     2521 gcttgagacg aagcttgaca cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2581 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2641 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2701 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2761 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2821 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2881 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2941 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3001 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3061 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3121 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3181 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3241 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3301 tgagattatc aaaaaggatc ttcacctaga tccttttgtg atgatcggca cgtaagaggt
     3361 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     3421 ttttcaggag ctaaggaagc taaaatgagc catattcaac gggaaacgtc ttgctcaagg
     3481 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     3541 gtcgggcaat caggtgcgac aatctaccga ttgtatggga agcccgatgc gccagagttg
     3601 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     3661 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     3721 gatgcatggt tactcaccac tgcgatccca gggaaaacag cattccaggt attagaagaa
     3781 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     3841 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgact cgctcaggcg
     3901 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     3961 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     4021 gtagtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     4081 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     4141 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     4201 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctga
     4261 ttttttttaa ggcagttatt ggtgccctta aacgcctggt tgctacgcct gaataagtga
     4321 taataagcgg atgaatggca gaaattcgat gataagctgt caaacatgat gttacgcgcc
     4381 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4441 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4501 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4561 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccagcgcc
     4621 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4681 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4741 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4801 ttttaacaaa atattaacgc ttacaatttt aacaagcttc gtct
//
