LOCUS       sequence-240494-        4808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for constitutive expression of RedF in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: hCMV-IE1:RedF
FEATURES             Location/Qualifiers
     source          1..4808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        39..342
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        343..546
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     497..517
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2227..2451
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2571..3159)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2651..2670)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3274..3376
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3377..4192
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3377..4192
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3450..3469)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4306..4761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4393..4412)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4603..4624
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
       61 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      121 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      181 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      241 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      301 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      361 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      421 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      481 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      541 agagctggtt tagtgaaccg tcagatcaag ctcaaatgga gaacatggag aacgacgaga
      601 acattgtggt gggacccaag ccgttctacc cgatcgagga gggttccgct ggcacccagc
      661 tgcgcaagta catggagcgc tacgctaagc tgggtgctat cgccttcacc aacgccgtga
      721 ccggtgtgga ttactcctac gccgagtacc tggagaagtc ctgctgcctc ggaaaggccc
      781 tgcagaacta cggtctggtc gtggatggcc gcattgccct ctgctcggag aactgcgagg
      841 agttcttcat tcccgtgatc gctggtctgt tcatcggagt cggcgttgcc ccaaccaacg
      901 agatctacac cctgcgcgag ctggtccaca gcctgggtat cagcaagccg accatcgttt
      961 tctcgagcaa gaagggcctc gacaaggtta tcaccgtgca aaaaaccgtc accaccatca
     1021 agaccatcgt tatcctggat tccaaggttg actaccgcgg ttaccagtgc ctcgacacct
     1081 tcatcaagcg taacacccca cccggcttcc aagcctcctc cttcaaaacc gtggaggttg
     1141 atcgcaagga gcaggtggcc ctgatcatga actcctccgg ctccaccggt ctgccaaagg
     1201 gcgtgcagct gacccacgag aacaccgtca cccgcttctc gcatgcccgt gatcccatct
     1261 acggcaacca ggtgtcccca ggcaccgccg tcctgaccgt cgtgcccctc caccacggct
     1321 tcggcatgtt caccactctc ggctacttga tctgcggctt ccgtgtggtg atgctgacta
     1381 agttcgacga ggagactttc ctcaagaccc tgcaagacta caagtgcact tacgtgatcc
     1441 tggtgccgac cctgttcgcc atcctgaaca agagcgagct gttgaacaaa tacgacctgt
     1501 cgaacctggt ggagatcgct tccggcggcg ccccactgtc gaaggaagtt ggcgaggccg
     1561 ttgctcgccg cttcaacctg cccggcgtcc gtcagggtta cggcctgacc gagactacct
     1621 cggctatcat catcaccccc gagggcgacg ataagcccgg cgcttccggc aaggttgttc
     1681 cactcttcaa agccaaggtg atcgacctgg acaccaagaa gtccctgggt cccaaccgtc
     1741 gcggagaagt gtgcgtcaag ggccccatgc tgatgaaggg ctacgtcaac aacccagagg
     1801 ccaccaagga gttgatcgac gaggagggct ggctgcacac tggtgacatc ggttactacg
     1861 acgaagagaa gcacttcttc atcgttgacc gcctgaagtc cttgatcaag tacaagggct
     1921 accaggttcc ccccgccgag ctcgagagcg tcctgctgca gcacccctcc attttcgatg
     1981 ccggagtggc cggtgtcccc gatcccgtgg ctggcgagct gccaggtgcc gtcgtcgtgc
     2041 tggagtccgg caagaacatg accgagaagg aagtgatgga ttacgtggcc tcgcaagtgt
     2101 ccaacgccaa gcgcctgcgc ggcggcgtgc gcttcgtgga tgaggtgccc aagggcctca
     2161 ccggaaagat cgacggtcgc gctatccgcg agatcctcaa gaagcccgtg gccaagatgt
     2221 gagcttctgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     2281 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     2341 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     2401 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcgcttgaga
     2461 cgaagcttga cacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2521 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2581 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2641 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2701 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2761 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2821 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2881 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2941 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3001 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3061 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3121 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3181 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3241 tcaaaaagga tcttcaccta gatccttttg tgatgatcgg cacgtaagag gttccaactt
     3301 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
     3361 agctaaggaa gctaaaatga gccatattca acgggaaacg tcttgctcaa ggccgcgatt
     3421 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     3481 atcaggtgcg acaatctacc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa
     3541 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     3601 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     3661 gttactcacc actgcgatcc cagggaaaac agcattccag gtattagaag aatatcctga
     3721 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     3781 tgtttgtaat tgtcctttta acagcgatcg cgtatttcga ctcgctcagg cgcaatcacg
     3841 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     3901 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtagtcac
     3961 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     4021 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     4081 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     4141 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct gatttttttt
     4201 aaggcagtta ttggtgccct taaacgcctg gttgctacgc ctgaataagt gataataagc
     4261 ggatgaatgg cagaaattcg atgataagct gtcaaacatg atgttacgcg ccctgtagcg
     4321 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4381 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4441 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4501 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccagcg ccctgataga
     4561 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4621 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4681 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     4741 aaatattaac gcttacaatt ttaacaagct tcgtctcagt caggagtagt tattaatagt
     4801 aatcaatt
//
