LOCUS       sequence-240497-        4097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for constitutive expression of Renilla in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: hCMV-IE1:Renilla
FEATURES             Location/Qualifiers
     source          1..4097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        29..332
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        333..536
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     487..507
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    1506..1730
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1850..2438)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1930..1949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2553..2655
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2656..3471
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2656..3471
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2729..2748)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3585..4040
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3672..3691)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3882..3903
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
       61 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      121 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      181 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      241 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      301 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      361 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      421 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      481 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
      541 tagtgaaccg tcagatcaag ctcaaatgac ttccaaggtt tatgaccccg agcaacgcaa
      601 gagaatgata actgggcctc agtggtgggc cagatgtaaa caaatgaatg tccttgatag
      661 tttcatcaat tactacgaca gtgaaaagca tgcagaaaac gccgtaattt ttttgcatgg
      721 caacgcagcc tcctcttatt tgtggcgaca cgtagtcccg catatagagc cggtagctag
      781 gtgtattatc cctgatctga tcggaatggg aaagagtggc aagtcaggga atggatctta
      841 tcgccttctt gaccactaca agtatctgac ggcttggttc gaactcttga atttgccgaa
      901 gaaaatcatc ttcgtgggtc acgattgggg agcctgtctc gcgttccatt attcctacga
      961 gcatcaggac aagataaagg caatcgttca cgccgagtct gtagtagatg taatcgagtc
     1021 ctgggacgaa tggccagata ttgaggagga tatagcattg attaaaagcg aggaagggga
     1081 aaagatggtg ctggagaaca acttctttgt ggaaacaatg cttccttcaa aaatcatgag
     1141 aaaactggaa cccgaggaat ttgccgctta tctcgaaccg tttaaagaaa agggagaagt
     1201 ccgcaggcca acactttcct ggccaagaga aatcccgctc gtcaaaggcg gaaagccgga
     1261 tgttgtgcaa atagtccgaa attacaacgc gtacctccgg gcctctgacg atctccctaa
     1321 aatgtttata gaatcagatc caggattttt tagcaacgct atcgtagaag gagcaaagaa
     1381 atttccaaat accgaatttg tcaaagtaaa gggactgcat ttcagtcaag aggacgctcc
     1441 agacgagatg ggcaaataca taaagtcctt tgtagagagg gtcctgaaga acgaacagtg
     1501 agcttctgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     1561 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     1621 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     1681 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cgcttgagac
     1741 gaagcttgac acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1801 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1861 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1921 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1981 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2041 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2101 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2161 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2221 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2281 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2341 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2401 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2461 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2521 caaaaaggat cttcacctag atccttttgt gatgatcggc acgtaagagg ttccaacttt
     2581 caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga
     2641 gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt cttgctcaag gccgcgatta
     2701 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2761 tcaggtgcga caatctaccg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2821 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2881 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2941 ttactcacca ctgcgatccc agggaaaaca gcattccagg tattagaaga atatcctgat
     3001 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     3061 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgac tcgctcaggc gcaatcacga
     3121 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     3181 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtagtcact
     3241 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     3301 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     3361 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     3421 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttctg atttttttta
     3481 aggcagttat tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg
     3541 gatgaatggc agaaattcga tgataagctg tcaaacatga tgttacgcgc cctgtagcgg
     3601 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3661 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3721 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3781 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccagcgc cctgatagac
     3841 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     3901 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     3961 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4021 aatattaacg cttacaattt taacaagctt cgtctcagtc aggagtagtt attaatagta
     4081 atcaattacg gggtcat
//
