LOCUS       sequence-240499-        4097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for constitutive expression of GrRenilla in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: hCMV-IE1:GrRenilla
FEATURES             Location/Qualifiers
     source          1..4097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      20..475
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(107..126)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     317..338
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        561..864
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        865..1068
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1019..1039
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2038..2262
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2382..2970)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2462..2481)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3085..3187
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3188..4003
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3188..4003
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3261..3280)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 agctgtcaaa catgatgtta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
       61 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      121 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      181 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      241 gatggttcac gtagtgggcc agcgccctga tagacggttt ttcgcccttt gacgttggag
      301 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
      361 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
      421 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aattttaaca
      481 agcttcgtct cagtcaggag tagttattaa tagtaatcaa ttacggggtc attagttcat
      541 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      601 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      661 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      721 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      781 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      841 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      901 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      961 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     1021 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
     1081 cgtcagatca agctcaaatg gcttccaaag tctacgaccc agaacaaagg aagaggatga
     1141 ttactggacc gcagtggtgg gcaaggtgca agcagatgaa tgtactcgat agttttatca
     1201 attattacga ctccgaaaaa cacgcggaaa atgctgtcat attcctgcac gggaacgcca
     1261 ctagcagcta cttgtggcgg cacgtagtcc cccacataga gcctgttgcc cgatgtatta
     1321 ttcccgatct cattggaatg ggcaaatcag gcaaatctgg gaacggttct taccgcctgc
     1381 tcgatcacta taaatatttg accgcttggt ttgagctgct taatctccct aaaaaaataa
     1441 tattcgttgg acacgactgg ggctcagctc tggcgttcca ctacgcatac gagcatcaag
     1501 accgaataaa agcaatagta catatggagt cagttgttga cgtcatagag tcatggatgg
     1561 gttggccaga tattgaagag gaattggccc tcatcaagag tgaggagggg gaaaaaatgg
     1621 tcctggaaaa taattttttc gtggaaacct tgctcccgag taaaataatg cgaaaacttg
     1681 aacccgaaga gtttgctgct tatttggagc ctttcaagga aaagggggaa gtaagacgac
     1741 caaccctttc ttggccgagg gagatacctc ttgttaaggg tggaaagccc gacgtagtcc
     1801 agatagtccg aaattacaac gcgtacctga gagctagtga tgacctccct aagttgttta
     1861 tcgagtcaga ccctgggttc ttcagcaacg caattgtaga aggagcgaaa aaatttccaa
     1921 atacagaatt tgtgaaggtt aaggggctcc actttttgca agaggacgcc cccgatgaga
     1981 tgggaaaata cattaaatct ttcgtagagc gcgtccttaa gaatgaacag tgagcttctg
     2041 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     2101 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     2161 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     2221 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcgcttgag acgaagcttg
     2281 acacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2341 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2401 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2461 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2521 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2581 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2641 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2701 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2761 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2821 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2881 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2941 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3001 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3061 atcttcacct agatcctttt gtgatgatcg gcacgtaaga ggttccaact ttcaccataa
     3121 tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga
     3181 agctaaaatg agccatattc aacgggaaac gtcttgctca aggccgcgat taaattccaa
     3241 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     3301 gacaatctac cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     3361 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     3421 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     3481 cactgcgatc ccagggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     3541 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     3601 ttgtcctttt aacagcgatc gcgtatttcg actcgctcag gcgcaatcac gaatgaataa
     3661 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     3721 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtagtca ctcatggtga
     3781 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     3841 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     3901 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     3961 gaataaattg cagtttcatt tgatgctcga tgagtttttc tgattttttt taaggcagtt
     4021 attggtgccc ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg
     4081 gcagaaattc gatgata
//
