LOCUS       sequence-240510-        8780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing HA-ATG13[3SD].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Chew LH, Lu S, Liu X, Li FK, Yu AY, Klionsky DJ, Dong MQ, Yip CK
  TITLE     Molecular interactions of the Saccharomyces cerevisiae Atg1 complex 
            provide insights into assembly and regulatory mechanisms.
  JOURNAL   Autophagy. 2015;11(6):891-905. doi: 10.1080/15548627.2015.1040972.
  PUBMED    25998554
REFERENCE   2  (bases 1 to 8780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):891-905. doi"; pages: " 
            10.1080/15548627.2015.1040972"
COMMENT     Sequence Label: pCuHA-ATG13[3SD](426)
FEATURES             Location/Qualifiers
     source          1..8780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..65
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    182..203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(218..223,224..241,242..248)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    256..272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     261..283
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     280..296
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     280..296
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     315..335
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        317..335
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        354..588
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     CDS             748..774
                     /codon_start=1
                     /product="synthetic cistron containing a ribosome binding 
                     site (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
                     /label=minicistron
                     /note="This first cistron should terminate 3 bp upstream of
                     the ATG for the second cistron."
                     /translation="MYRLNKEE"
     CDS             790..807
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..843
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(847..855,856..870)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             907..996
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(3244..3260)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3245..3261)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      3263..3510
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3279..3297)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(3528..3547)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3529..3547)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3557..3574)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3557..3573)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3566..3588)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3714..4169
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3801..3820)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4011..4032
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4300..5103)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5104..5319)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5343..5362)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5462..5484
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5522..5540)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      5581..6923
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    5913..5960
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        6950..7054
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7055..7123,7124..7915)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7273..7292)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8086..8674
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8575..8594
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
       61 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
      121 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      181 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      241 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
      301 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctcctagtta
      361 gaaaaagaca tttttgctgt cagtcactgt caagagattc ttttgctggc atttcttcta
      421 gaagcaaaaa gagcgatgcg tcttttccgc tgaaccgttc cagcaaaaaa gactaccaac
      481 gcaatatgga ttgtcagaat cgtataaaag agaagcaaat aactccttgt cttgtatcaa
      541 ttgcattata gtatcttctt gttagtgcaa tatcatatag aagtcatcga ctagtggatc
      601 ccccgggctg caggaattcg atatcaagct tcgctgataa aaagcgaagc ggcactgctc
      661 tttaacaatt tatcagacaa tctgtgtggg cactcgaccg gaattatcga ttaactttat
      721 tattaaaaat taaagaggta tatattaatg tatcgattaa ataaggagga ataaaccatg
      781 gggggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg
      841 ggtcgggatc tgtacgacga tgacgataag gatccgagct cgagatctgc acctagatcc
      901 atcttttacc catacgatgt tcctgactat gcgggctatc cctatgacgt cccggactat
      961 gcagggtcct atccatatga cgttccagat tacgctgctc agtgcggatc tagggttgcc
     1021 gaagaggaca tcgagaagca agtccttcaa ttgatagaca gcttttttct gaagactaca
     1081 ctactaatat gctccaccga atcaagtcga taccagtctt ctacagaaaa tatattccta
     1141 tttgacgaca catggtttga agatcactca gaattagtga gtgagctacc cgagataata
     1201 tcaaaatggt ctcactacga tggtcgaaaa gagttgccac ccttagtggt agagacatat
     1261 ttggatttaa gacagttaaa ctcgtctcat ttagttagat taaaggacca cgaaggccat
     1321 ttgtggaacg tttgcaaagg aactaagaag caggaaatcg tgatggaacg ttggcttatc
     1381 gaattagata attcatcccc aactttcaaa tcatacagtg aagatgagac tgatgttaat
     1441 gaactttcta aacagctagt ccttctcttc cgttatttgt tgactttaat acagttacta
     1501 cccacaacag aattatacca attattaata aagtcttata acggcccgca aaatgaagga
     1561 agttccaatc caataacttc cacgggccca ctagtaagta tccggacgtg tgtccttgac
     1621 ggatctaaac caattttatc gaaggggaga atagggttga gcaaaccgat tattaataca
     1681 tattccaatg cgcttaacga atcaaacctg ccagcccatt tagatcaaaa gaagatcaca
     1741 cctgtatgga caaagtttgg actcttaaga gtctcggtat catacagacg tgattggaag
     1801 tttgaaatta acaatacaaa cgacgaatta ttttcagctc gacatgcatc tgtctcacat
     1861 aactcacaag gaccccagaa tcagccagaa caagaaggac aaagtgatca agacataggg
     1921 aaacgccaac cacaatttca acagcagcag cagccccaac agcagcagca gcagcagcaa
     1981 cagcaacaga gacaacacca ggtccagaca caacaacaaa gacagatacc tgataggaga
     2041 tctctttcac tttctccttg tacaagagcc aattcttttg aaccacaatc ttggcagaag
     2101 aaagtctatc caatatcgag acctgttcaa ccatttaaag ttggttcaat tggaagtcaa
     2161 agtgcgagca gaaatccctc taattcatcg tttttcaacc aaccacctgt tcataggcca
     2221 agtatgagct ccaactacgg gccacaaatg aatattgaag gtaccagtgt tggaagcacc
     2281 tcaaagtatt cctcctcctt tgggaacatt cgtcgtcact caagtgtaaa gacgacagag
     2341 aatgctgaaa aagtatcaaa agctgtaaag agcccactac aacctcaaga atcacaagaa
     2401 gatttaatgg attttgttaa attactcgaa gaaaaacccg atctaactat aaagaagaca
     2461 agtggaaata atccacccaa tatcaatatt tctgattctc taatcagata tcagaatttg
     2521 aagccaagta atggcttatt aagtgaagat ttatccgtaa gtttatccat ggatccaaat
     2581 catacatatc acagaggcag atcagattcc cactcaccat tgccttcaat atccccttcg
     2641 atgtattatg gatcgttgaa ctcgagaatg tctcaaggcg ccaatgcaag ccatttgatt
     2701 gcaagaggcg gtgggaattc atctactagt gccttgaata gtagaaggaa ttctttagat
     2761 aagagctcaa acaagcaggg tatgtcaggc ttacctccta tttttggtgg agagagtact
     2821 tcatatcacc acgacaacaa aatacaaaag tacaaccaat taggagtaga agaagatgat
     2881 gatgacgaga atgaccgttt gctcaaccaa atgggaaaca gtgctacaaa attcaaaagt
     2941 tcaatagacc caagagacat tgatgacatt tcaagttctt tcataaaaag taggatacct
     3001 atcagacaac cataccatta ctctcaacca actactgcgc cctttcaagc tcaggcgaaa
     3061 tttcataaac ctgcaaataa gttaatcgat aatggtaata ggagtaatag taacaataac
     3121 aatcataatg ggaatgatgc agttggtgtg atgcataatg acgaggatga tcaagatgat
     3181 gatctagtat ttttcatgag tgatatgaac ctttctaaag aaggttaaaa atggtaagct
     3241 tatcgatacc gtcgacctcg agtcatgtaa ttagttatgt cacgcttaca ttcacgccct
     3301 ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct
     3361 atttattttt ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt
     3421 ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag
     3481 gttttgggac gctcgaaggc tttaatttgc ggccggtacc caattcgccc tatagtgagt
     3541 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     3601 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     3661 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc
     3721 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3781 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3841 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3901 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     3961 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4021 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4081 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4141 ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg
     4201 tgcggtattt cacaccgcat agggtaataa ctgatataat taaattgaag ctctaatttg
     4261 tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct
     4321 tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct
     4381 tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc
     4441 atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg
     4501 tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa
     4561 gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc
     4621 agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt
     4681 tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg
     4741 tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt
     4801 gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc
     4861 ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac
     4921 atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt
     4981 ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa
     5041 tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga
     5101 catgatttat cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta agaatactgg
     5161 gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc
     5221 cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc aaggaaaccg
     5281 aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa aggtggtatg gtgcactctc
     5341 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     5401 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     5461 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     5521 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagtat
     5581 gatccaatat caaaggaaat gatagcattg aaggatgaga ctaatccaat tgaggagtgg
     5641 cagcatatag aacagctaaa gggtagtgct gaaggaagca tacgataccc cgcatggaat
     5701 gggataatat cacaggaggt actagactac ctttcatcct acataaatag acgcatataa
     5761 gtacgcattt aagcataaac acgcactatg ccgttcttct catgtatata tatatacagg
     5821 caacacgcag atataggtgc gacgtgaaca gtgagctgta tgtgcgcagc tcgcgttgca
     5881 ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc tattccgaag ttcctattct
     5941 ctagaaagta taggaacttc agagcgcttt tgaaaaccaa aagcgctctg aagacgcact
     6001 ttcaaaaaac caaaaacgca ccggactgta acgagctact aaaatattgc gaataccgct
     6061 tccacaaaca ttgctcaaaa gtatctcttt gctatatatc tctgtgctat atccctatat
     6121 aacctaccca tccacctttc gctccttgaa cttgcatcta aactcgacct ctacattttt
     6181 tatgtttatc tctagtatta ctctttagac aaaaaaattg tagtaagaac tattcataga
     6241 gtgaatcgaa aacaatacga aaatgtaaac atttcctata cgtagtatat agagacaaaa
     6301 tagaagaaac cgttcataat tttctgacca atgaagaatc atcaacgcta tcactttctg
     6361 ttcacaaagt atgcgcaatc cacatcggta tagaatataa tcggggatgc ctttatcttg
     6421 aaaaaatgca cccgcagctt cgctagtaat cagtaaacgc gggaagtgga gtcaggcttt
     6481 ttttatggaa gagaaaatag acaccaaagt agccttcttc taaccttaac ggacctacag
     6541 tgcaaaaagt tatcaagaga ctgcattata gagcgcacaa aggagaaaaa aagtaatcta
     6601 agatgctttg ttagaaaaat agcgctctcg ggatgcattt ttgtagaaca aaaaagaagt
     6661 atagattctt tgttggtaaa atagcgctct cgcgttgcat ttctgttctg taaaaatgca
     6721 gctcagattc tttgtttgaa aaattagcgc tctcgcgttg catttttgtt ttacaaaaat
     6781 gaagcacaga ttcttcgttg gtaaaatagc gctttcgcgt tgcatttctg ttctgtaaaa
     6841 atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt ttgttctaca
     6901 aaatgaagca cagatgcttc gttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6961 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7021 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7081 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7141 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7201 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7261 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     7321 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7381 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7441 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7501 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7561 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     7621 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7681 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     7741 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     7801 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     7861 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     7921 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     7981 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8041 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8101 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8161 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8221 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8281 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8341 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8401 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8461 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8521 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8581 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8641 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8701 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8761 tgtggataac cgtattaccg
//
