LOCUS       sequence-240519-        8780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing HA-ATG13[3SA].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Chew LH, Lu S, Liu X, Li FK, Yu AY, Klionsky DJ, Dong MQ, Yip CK
  TITLE     Molecular interactions of the Saccharomyces cerevisiae Atg1 complex 
            provide insights into assembly and regulatory mechanisms.
  JOURNAL   Autophagy. 2015;11(6):891-905. doi: 10.1080/15548627.2015.1040972.
  PUBMED    25998554
REFERENCE   2  (bases 1 to 8780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):891-905. doi"; pages: " 
            10.1080/15548627.2015.1040972"
COMMENT     Sequence Label: pCuHA-ATG13[3SA](426)
FEATURES             Location/Qualifiers
     source          1..8780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     313..332
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     566..583
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    700..721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(736..741,742..759,760..766)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    774..790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     779..801
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     798..814
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     798..814
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     833..853
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        835..853
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        872..1106
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     CDS             1266..1292
                     /codon_start=1
                     /product="synthetic cistron containing a ribosome binding 
                     site (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
                     /label=minicistron
                     /note="This first cistron should terminate 3 bp upstream of
                     the ATG for the second cistron."
                     /translation="MYRLNKEE"
     CDS             1308..1325
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1329..1361
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(1365..1373,1374..1388)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             1425..1514
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(3762..3778)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3763..3779)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      3781..4028
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3797..3815)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(4046..4065)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4047..4065)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4075..4092)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4075..4091)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4084..4106)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4232..4687
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4319..4338)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4529..4550
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4818..5621)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5622..5837)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5861..5880)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5980..6002
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6040..6058)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      6099..7441
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    6431..6478
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        7468..7572
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7573..7641,7642..8433)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7791..7810)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8604..8780,1..412)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
       61 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      121 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      181 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      241 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      301 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      361 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      421 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      481 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      541 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      601 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      661 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      721 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      781 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
      841 ccctcactaa agggaacaaa agctggagct cctagttaga aaaagacatt tttgctgtca
      901 gtcactgtca agagattctt ttgctggcat ttcttctaga agcaaaaaga gcgatgcgtc
      961 ttttccgctg aaccgttcca gcaaaaaaga ctaccaacgc aatatggatt gtcagaatcg
     1021 tataaaagag aagcaaataa ctccttgtct tgtatcaatt gcattatagt atcttcttgt
     1081 tagtgcaata tcatatagaa gtcatcgact agtggatccc ccgggctgca ggaattcgat
     1141 atcaagcttc gctgataaaa agcgaagcgg cactgctctt taacaattta tcagacaatc
     1201 tgtgtgggca ctcgaccgga attatcgatt aactttatta ttaaaaatta aagaggtata
     1261 tattaatgta tcgattaaat aaggaggaat aaaccatggg gggttctcat catcatcatc
     1321 atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg tacgacgatg
     1381 acgataagga tccgagctcg agatctgcac ctagatccat cttttaccca tacgatgttc
     1441 ctgactatgc gggctatccc tatgacgtcc cggactatgc agggtcctat ccatatgacg
     1501 ttccagatta cgctgctcag tgcggatcta gggttgccga agaggacatc gagaagcaag
     1561 tccttcaatt gatagacagc ttttttctga agactacact actaatatgc tccaccgaat
     1621 caagtcgata ccagtcttct acagaaaata tattcctatt tgacgacaca tggtttgaag
     1681 atcactcaga attagtgagt gagctacccg agataatatc aaaatggtct cactacgatg
     1741 gtcgaaaaga gttgccaccc ttagtggtag agacatattt ggatttaaga cagttaaact
     1801 cgtctcattt agttagatta aaggaccacg aaggccattt gtggaacgtt tgcaaaggaa
     1861 ctaagaagca ggaaatcgtg atggaacgtt ggcttatcga attagataat tcatccccaa
     1921 ctttcaaatc atacagtgaa gatgagactg atgttaatga actttctaaa cagctagtcc
     1981 ttctcttccg ttatttgttg actttaatac agttactacc cacaacagaa ttataccaat
     2041 tattaataaa gtcttataac ggcccgcaaa atgaaggaag ttccaatcca ataacttcca
     2101 cgggcccact agtaagtatc cggacgtgtg tccttgacgg atctaaacca attttatcga
     2161 aggggagaat agggttgagc aaaccgatta ttaatacata ttccaatgcg cttaacgaat
     2221 caaacctgcc agcccattta gatcaaaaga agatcacacc tgtatggaca aagtttggac
     2281 tcttaagagt ctcggtatca tacagacgtg attggaagtt tgaaattaac aatacaaacg
     2341 acgaattatt ttcagctcga catgcatctg tctcacataa ctcacaagga ccccagaatc
     2401 agccagaaca agaaggacaa agtgatcaag acatagggaa acgccaacca caatttcaac
     2461 agcagcagca gccccaacag cagcagcagc agcagcaaca gcaacagaga caacaccagg
     2521 tccagacaca acaacaaaga cagatacctg ataggagatc tctttcactt tctccttgta
     2581 caagagccaa ttcttttgaa ccacaatctt ggcagaagaa agtctatcca atatcgagac
     2641 ctgttcaacc atttaaagtt ggttcaattg gaagtcaaag tgcgagcaga aatccctcta
     2701 attcatcgtt tttcaaccaa ccacctgttc ataggccaag tatgagctcc aactacgggc
     2761 cacaaatgaa tattgaaggt accagtgtcg gaagcacctc aaagtattcc tcctcctttg
     2821 ggaacattcg tcgtcactca agtgtaaaga cgacagagaa tgctgaaaaa gtatcaaaag
     2881 ctgtaaagag cccactacaa cctcaagaat cacaagaaga tttaatggat tttgttaaat
     2941 tactcgaaga aaaacccgat ctaactataa agaagacaag tggaaataat ccacccaata
     3001 tcaatatttc tgattctcta atcagatatc agaatttgaa gccaagtaat ggcttattaa
     3061 gtgaagattt atccgtaagt ttatccatgg atccaaatca tacatatcac agaggcagat
     3121 cagattccca ctcaccattg ccttcaatat ccccttcgat gtattatgga tcgttgaact
     3181 cgagaatgtc tcaaggcgcc aatgcaagcc atttgattgc aagaggcggt gggaattcat
     3241 ctactagtgc cttgaatagt agaaggaatt ctttagataa gagctcaaac aagcagggta
     3301 tgtcaggctt acctcctatt tttggtggag agagtacttc atatcaccac gacaacaaaa
     3361 tacaaaagta caaccaatta ggagtagaag aagatgatga tgacgagaat gaccgtttgc
     3421 tcaaccaaat gggaaacagt gctacaaaat tcaaaagttc aatagcacca agagcaattg
     3481 atgccatttc aagttctttc ataaaaagta ggatacctat cagacaacca taccattact
     3541 ctcaaccaac tactgcgccc tttcaagctc aggcgaaatt tcataaacct gcaaataagt
     3601 taatcgataa tggtaatagg agtaatagta acaataacaa tcataatggg aatgatgcag
     3661 ttggtgtgat gcataatgac gaggatgatc aagatgatga tctagtattt ttcatgagtg
     3721 atatgaacct ttctaaagaa ggttaaaaat ggtaagctta tcgataccgt cgacctcgag
     3781 tcatgtaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg
     3841 aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt
     3901 tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt
     3961 acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt
     4021 taatttgcgg ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     4081 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     4141 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4201 ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg
     4261 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4321 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4381 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4441 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4501 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4561 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4621 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     4681 acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag
     4741 ggtaataact gatataatta aattgaagct ctaatttgtg agtttagtat acatgcattt
     4801 acttataata cagtttttta gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct
     4861 gcttttctgt aacgttcacc ctctacctta gcatcccttc cctttgcaaa tagtcctctt
     4921 ccaacaataa taatgtcaga tcctgtagag accacatcat ccacggttct atactgttga
     4981 cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa
     5041 ccttcatctc ttccacccat gtctctttga gcaataaagc cgataacaaa atctttgtcg
     5101 ctcttcgcaa tgtcaacagt acccttagta tattctccag tagataggga gcccttgcat
     5161 gacaattctg ctaacatcaa aaggcctcta ggttcctttg ttacttcttc tgccgcctgc
     5221 ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg cattcgtaat gtctgcccat
     5281 tctgctattc tgtatacacc cgcagagtac tgcaatttga ctgtattacc aatgtcagca
     5341 aattttctgt cttcgaagag taaaaaattg tacttggcgg ataatgcctt tagcggctta
     5401 actgtgccct ccatggaaaa atcagtcaag atatccacat gtgtttttag taaacaaatt
     5461 ttgggaccta atgcttcaac taactccagt aattccttgg tggtacgaac atccaatgaa
     5521 gcacacaagt ttgtttgctt ttcgtgcatg atattaaata gcttggcagc aacaggacta
     5581 ggatgagtag cagcacgttc cttatatgta gctttcgaca tgatttatct tcgtttcctg
     5641 caggtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt ttcttcaaca
     5701 ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt
     5761 tcggagatta ccgaatcaaa aaaatttcaa ggaaaccgaa atcaaaaaaa agaataaaaa
     5821 aaaaatgatg aattgaaaag gtggtatggt gcactctcag tacaatctgc tctgatgccg
     5881 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     5941 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     6001 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     6061 tataggttaa tgtcatgata ataatggttt cttagtatga tccaatatca aaggaaatga
     6121 tagcattgaa ggatgagact aatccaattg aggagtggca gcatatagaa cagctaaagg
     6181 gtagtgctga aggaagcata cgataccccg catggaatgg gataatatca caggaggtac
     6241 tagactacct ttcatcctac ataaatagac gcatataagt acgcatttaa gcataaacac
     6301 gcactatgcc gttcttctca tgtatatata tatacaggca acacgcagat ataggtgcga
     6361 cgtgaacagt gagctgtatg tgcgcagctc gcgttgcatt ttcggaagcg ctcgttttcg
     6421 gaaacgcttt gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcag
     6481 agcgcttttg aaaaccaaaa gcgctctgaa gacgcacttt caaaaaacca aaaacgcacc
     6541 ggactgtaac gagctactaa aatattgcga ataccgcttc cacaaacatt gctcaaaagt
     6601 atctctttgc tatatatctc tgtgctatat ccctatataa cctacccatc cacctttcgc
     6661 tccttgaact tgcatctaaa ctcgacctct acatttttta tgtttatctc tagtattact
     6721 ctttagacaa aaaaattgta gtaagaacta ttcatagagt gaatcgaaaa caatacgaaa
     6781 atgtaaacat ttcctatacg tagtatatag agacaaaata gaagaaaccg ttcataattt
     6841 tctgaccaat gaagaatcat caacgctatc actttctgtt cacaaagtat gcgcaatcca
     6901 catcggtata gaatataatc ggggatgcct ttatcttgaa aaaatgcacc cgcagcttcg
     6961 ctagtaatca gtaaacgcgg gaagtggagt caggcttttt ttatggaaga gaaaatagac
     7021 accaaagtag ccttcttcta accttaacgg acctacagtg caaaaagtta tcaagagact
     7081 gcattataga gcgcacaaag gagaaaaaaa gtaatctaag atgctttgtt agaaaaatag
     7141 cgctctcggg atgcattttt gtagaacaaa aaagaagtat agattctttg ttggtaaaat
     7201 agcgctctcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa
     7261 attagcgctc tcgcgttgca tttttgtttt acaaaaatga agcacagatt cttcgttggt
     7321 aaaatagcgc tttcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt
     7381 gaaaaattag cgctctcgcg ttgcattttt gttctacaaa atgaagcaca gatgcttcgt
     7441 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7501 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7561 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7621 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7681 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7741 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7801 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7861 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7921 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7981 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8041 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     8101 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     8161 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8221 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8281 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8341 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8401 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8461 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8521 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8581 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8641 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8701 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8761 tagccgtagt taggccacca
//
