LOCUS       sequence-240563-        1488 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses residues 1-199 of CHMP1B from a His-SUMO bacterial 
            expression vector. Internal ID: WISP 18-24.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Nguyen HC, Talledge N, McCullough J, Sharma A, Moss FR 3rd, Iwasa 
            JH, Vershinin MD, Sundquist WI, Frost A
  TITLE     Membrane constriction and thinning by sequential ESCRT-III 
            polymerization.
  JOURNAL   Nat Struct Mol Biol. 2020 Apr;27(4):392-399. doi: 
            10.1038/s41594-020-0404-x. Epub 2020 Apr 6.
  PUBMED    32251413
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0404-x"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-04"; volume: "27"; issue: "4"; pages: "392-399"
COMMENT     Sequence Label: pCA528 CHMP1B 1-199
FEATURES             Location/Qualifiers
     source          1..1488
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     16..35
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(55..132)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     338..357
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     441..460
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        441..459
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    460..484
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             499..521
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             535..552
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             556..846
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac
       61 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca
      121 ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc
      181 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga
      241 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
      301 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
      361 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
      421 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
      481 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gggtcatcac
      541 catcatcacc atggttcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca
      601 gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc
      661 ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag
      721 ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag
      781 acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt
      841 ggtgggatga gcaacatgga gaaacacctg ttcaacctga agttcgcggc caaagaactg
      901 agtaggagtg ccaaaaaatg cgataaggag gaaaaggccg aaaaggccaa aattgaaaag
      961 gccattcaga agggcaacat ggaagttgcg aggatacacg ccgaaaatgc catccgccag
     1021 aagaaccagg cggtgaattt cttgagaatg agtgcgcgag tcgatgcagt ggctgccagg
     1081 gtccagacgg cggtgacgat gggcaaggtg accaagtcga tggctggtgt ggttaagtcg
     1141 atggatgcga cattgaagac catgaatctg gagaagattt ctgctttgat ggacaaattc
     1201 gagcaccagt ttgagactct ggacgtccag acgcagcaaa tggaagacac gatgagcagc
     1261 acgacgacgc tcaccactcc ccagaaccaa gtggatatgc tgctccagga aatggcagat
     1321 gaggcgggcc tcgacctcaa catggagctg ccgcagggcc agaccggctc cgtgggcacg
     1381 agcgtggctt cggcggagca ggatgaactg tctcagagac tggcccgcct tcgggatcaa
     1441 gtgtaagaac cggctgctaa caaagcccga aagaagctat tgtgtcac
//
