LOCUS       sequence-240572-       12805 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of DAPK1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12805)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-DAPK1
FEATURES             Location/Qualifiers
     source          1..12805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7742..7766)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7819..7860
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7881..8448
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7898..8450
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8042..8059)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8269..8288
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8462..8464,8465..8467,8468..9181)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8462..8464,8465..8467,8468..9178)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8507..8528)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8768..8787)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    9195..9783
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9248..9268)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9666..9677)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9858..10091
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10210..10228)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10296..10400
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10401..10469,10470..11261)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10619..10638)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11432..12020
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11921..11940
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12174..12191
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12431..12452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12467..12472,12473..12490,12491..12497)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12505..12521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12510..12532
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12529..12545
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12529..12545
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12595..12614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg accgtgttca ggcaggaaaa cgtggatgat
     3481 tactacgaca ccggcgagga acttggcagt ggacagtttg cggttgtgaa gaaatgccgt
     3541 gagaaaagca ccggcctcca gtatgccgcc aaattcatca agaaaaggag gactaagtcc
     3601 agccggcggg gtgtgagccg cgaggacatc gagcgggagg tcagcatcct gaaggagatc
     3661 cagcacccca atgtcatcac cctgcacgag gtctatgaga acaagacgga cgtcatcctg
     3721 atcttggaac tcgttgcagg tggcgagctg tttgacttct tagctgaaaa ggaatcttta
     3781 actgaagagg aagcaactga atttctcaaa caaattctta atggtgttta ctacctgcac
     3841 tcccttcaaa tcgcccactt tgatcttaag cctgagaaca taatgctttt ggatagaaat
     3901 gtccccaaac ctcggatcaa gatcattgac tttgggttgg cccataaaat tgactttgga
     3961 aatgaattta aaaacatatt tgggactcca gagtttgtcg ctcctgagat agtcaactat
     4021 gaacctcttg gtcttgaggc agatatgtgg agtatcgggg taataaccta tatcctccta
     4081 agtggggcct ccccatttct tggagacact aagcaagaaa cgttagcaaa tgtatccgct
     4141 gtcaactacg aatttgagga tgaatacttc agtaatacca gtgccctagc caaagatttc
     4201 ataagaagac ttctggtcaa ggatccaaag aagagaatga caattcaaga tagtttgcag
     4261 catccctgga tcaagcctaa agatacacaa caggcactta gtagaaaagc atcagcagta
     4321 aacatggaga aattcaagaa gtttgcagcc cggaaaaaat ggaaacaatc cgttcgcttg
     4381 atatcactgt gccaaagatt atccaggtca ttcctgtcca gaagtaacat gagtgttgcc
     4441 agaagcgatg atactctgga tgaggaagac tcctttgtga tgaaagccat catccatgcc
     4501 atcaacgatg acaatgtccc aggcctgcag caccttctgg gctcattatc caactatgat
     4561 gttaaccaac ccaacaagca cgggacacct ccattactca ttgctgctgg ctgtgggaat
     4621 attcaaatac tacagttgct cattaaaaga ggctcgagaa tcgatgtcca ggataagggc
     4681 gggtccaatg ccgtctactg ggctgctcgg catggccacg tcgatacctt gaaatttctc
     4741 agtgagaaca aatgcccttt ggatgtgaaa gacaagtctg gagagatggc cctccacgtg
     4801 gcagctcgct atggccatgc tgacgtggct cagttactgt gcagcttcgg ctcaaatccc
     4861 aatatccagg acaaggaaga agaaaccccc ctgcactgtg ctgcttggca cggctattac
     4921 tctgtggcca aagccctttg tgaagccggc tgtaacgtga acatcaagaa ccgagaagga
     4981 gagacgcccc tcctgacagc ctctgccagg ggctaccacg acatcgtgga gtgtctggcc
     5041 gaacatggag ccgaccttaa tgcttgcgac aaggacggac acattgccct tcatctggct
     5101 gtaagacggt gtcagatgga ggtaatcaag actctcctca gccaagggtg tttcgtcgat
     5161 tatcaagaca ggcacggcaa tactcccctc catgtggcat gtaaagatgg caacatgcct
     5221 atcgtggtgg ccctctgtga agcaaactgc aatttggaca tctccaacaa gtatgggcga
     5281 acgcctctgc accttgcggc caacaacgga atcctagacg tggtccggta tctctgtctg
     5341 atgggagcca gcgttgaggc gctgaccacg gacggaaaga cggcagaaga tcttgctaga
     5401 tcggaacagc acgagcacgt agcaggtctc cttgcaagac ttcgaaagga tacgcaccga
     5461 ggactcttca tccagcagct ccgacccaca cagaacctgc agccaagaat taagctcaag
     5521 ctgtttggcc actcgggatc cgggaaaacc acccttgtag aatctctcaa gtgtgggctg
     5581 ctgaggagct ttttcagaag gcgtcggccc agactgtctt ccaccaactc cagcaggttc
     5641 ccaccttcac ccctggcttc taagcccaca gtctcagtga gcatcaacaa cctgtaccca
     5701 ggctgcgaga acgtgagtgt gaggagccgc agcatgatgt tcgagccggg tcttaccaaa
     5761 gggatgctgg aggtgtttgt ggccccgacc caccacccgc actgctcggc cgatgaccag
     5821 tccaccaagg ccatcgacat ccagaacgct tatttgaatg gagttggcga tttcagcgtg
     5881 tgggagttct ctggaaatcc tgtgtatttc tgctgttatg actattttgc tgcaaatgat
     5941 cccacgtcaa tccatgttgt tgtctttagt ctagaagagc cctatgagat ccagctgaac
     6001 caagtgattt tctggctcag tttcctgaag tcccttgtcc cagttgaaga acccatagcc
     6061 ttcggtggca agctgaagaa cccactccaa gttgtcctgg tggccaccca cgctgacatc
     6121 atgaatgttc ctcgaccggc tggaggcgag tttggatatg acaaagacac atcgttgctg
     6181 aaagagatta ggaacaggtt tggaaatgat cttcacattt caaataagct gtttgttctg
     6241 gatgctgggg cttctgggtc aaaggacatg aaggtacttc gaaatcatct gcaagaaata
     6301 cgaagccaga ttgtttcggt ctgtcctccc atgactcacc tgtgtgagaa aatcatctcc
     6361 acgctgcctt cctggaggaa gctcaatgga cccaaccagc tgatgtcgct gcagcagttt
     6421 gtgtacgacg tgcaggacca gctgaacccc ctggccagcg aggaggacct caggcgcatt
     6481 gctcagcagc tccacagcac aggcgagatc aacatcatgc aaagtgaaac agttcaggac
     6541 gtgctgctcc tggacccccg ctggctctgc acaaacgtcc tggggaagtt gctgtccgtg
     6601 gagaccccac gggcgctgca ccactaccgg ggccgctaca ccgtggagga catccagcgc
     6661 ctggtgcccg acagcgacgt ggaggagctg ctgcagatcc tcgatgccat ggacatctgc
     6721 gcccgggacc tgagcagcgg gaccatggtg gacgtcccag ccctgatcaa gacagacaac
     6781 ctgcaccgct cctgggctga tgaggaggac gaggtgatgg tgtatggtgg cgtgcgcatc
     6841 gtgcccgtgg aacacctcac ccccttccca tgtggcatct ttcacaaggt ccaggtgaac
     6901 ctgtgccggt ggatccacca gcaaagcaca gagggcgacg cggacatccg cctgtgggtg
     6961 aatggctgca agctggccaa ccgtggggcc gagctgctgg tgctgctggt caaccacggc
     7021 cagggcattg aggtccaggt ccgcggcctg gagacggaaa agatcaagtg ctgcctgctg
     7081 ctggactcgg tgtgcagcac cattgagaac gtcatggcca ccacgctgcc agggctcctg
     7141 accgtgaagc attacctgag cccccagcag ctgcgggagc accatgagcc cgtcatgatc
     7201 taccagccac gggacttctt ccgggcacag actctgaagg aaacctcact gaccaacacc
     7261 atgggggggt acaaggaaag cttcagcagc atcatgtgct tcgggtgtca cgacgtctac
     7321 tcacaggcca gcctcggcat ggacatccat gcatcagacc tgaacctcct cactcggagg
     7381 aaactgagtc gcctgctgga cccgcccgac cccctgggga aggactggtg ccttctcgcc
     7441 atgaacttag gcctccctga cctcgtggca aagtacaaca ccagtaacgg ggctcccaag
     7501 gatttcctcc ccagccccct ccacgccctg ctgcgggaat ggaccaccta ccctgagagc
     7561 acagtgggca ccctcatgtc caaactgagg gagctgggtc gccgggatgc cgcagacttt
     7621 ttgctgaagg catcctctgt gttcaaaatc aacctggatg gcaatggcca ggaggcctat
     7681 gcctcgagct gcaacagcgg cacctcttac aattccatta gctctgttgt atcccggtag
     7741 aacccagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcgagtcta
     7801 gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
     7861 cgtaccggtt agtaatgaga tccctccccc ccccctaacg ttactggccg aagccgcttg
     7921 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     7981 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     8041 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     8101 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     8161 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     8221 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     8281 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     8341 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     8401 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaca
     8461 tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     8521 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     8581 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     8641 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     8701 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     8761 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     8821 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     8881 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     8941 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     9001 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     9061 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     9121 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     9181 aatcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     9241 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     9301 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     9361 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     9421 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     9481 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     9541 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     9601 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     9661 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     9721 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     9781 cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     9841 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     9901 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     9961 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    10021 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    10081 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
    10141 atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat cattcttgaa
    10201 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
    10261 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
    10321 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    10381 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
    10441 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    10501 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    10561 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    10621 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
    10681 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    10741 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    10801 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    10861 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    10921 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    10981 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    11041 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    11101 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    11161 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    11221 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    11281 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    11341 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    11401 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    11461 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    11521 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    11581 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    11641 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    11701 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    11761 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    11821 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    11881 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    11941 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    12001 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    12061 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    12121 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    12181 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    12241 cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga
    12301 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    12361 cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    12421 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    12481 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    12541 atgacatgat tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt
    12601 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    12661 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    12721 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    12781 aaatatgtac tacaaactta gtagt
//
