LOCUS       sequence-240579-        9232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression, Genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Teng F, Li J, Cui T, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou
            Q, Li W
  TITLE     Enhanced mammalian genome editing by new Cas12a orthologs with 
            optimized crRNA scaffolds.
  JOURNAL   Genome Biol. 2019 Feb 5;20(1):15. doi: 10.1186/s13059-019-1620-8.
  PUBMED    30717767
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2019-02-5"; volume: "20(1):15. doi"; pages: " 
            10.1186/s13059-019-1620-8"
COMMENT     Sequence Label: pCAG-AsCas12a-2AeGFP
FEATURES             Location/Qualifiers
     source          1..9232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          665..1681
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1605..1627
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1689..1708
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1742..1760
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1772..1792
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1799..5719
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      5439..5448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5726..5746
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5753..5842
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             5864..5917
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5918..5920,5921..5923,5924..6637)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5918..5920,5921..5923,5924..6634)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5963..5984)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6224..6243)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6571..6592
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6680..6801
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6717..6736)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6771..6790
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6844..6860)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6844..6860)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6857..6879)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6868..6884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6892..6898,6899..6916,6917..6922))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6937..6958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7075..7092)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7246..7834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7326..7345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8005..8796,8797..8865))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8628..8647
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8866..8970)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9049..9068)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
      421 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
      481 gcagcgatgg gggcgggggg ggggggggsg cgcgccrggs ggggsggggs ggggsgrggg
      541 gsggggsggg gcgrggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      841 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
      901 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1021 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1081 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1141 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1201 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1261 ggcggggccg cctcgggccg gggrgggctc gggggagggg cgcggcggcc cccggagcgc
     1321 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1381 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1441 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1501 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1561 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagcg cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag ctagtgaatt
     1741 ctaatacgac tcactatagg ccgccaccat gcccaagaag aagaggaagg ttcccgggat
     1801 gacacagttc gagggcttta ccaacctgta tcaggtgagc aagacactgc ggtttgagct
     1861 gatcccacag ggcaagaccc tgaagcacat ccaggagcag ggcttcatcg aggaggacaa
     1921 ggcccgcaat gatcactaca aggagctgaa gcccatcatc gatcggatct acaagaccta
     1981 tgccgaccag tgcctgcagc tggtgcagct ggattgggag aacctgagcg ccgccatcga
     2041 ctcctataga aaggagaaaa ccgaggagac aaggaacgcc ctgatcgagg agcaggccac
     2101 atatcgcaat gccatccacg actacttcat cggccggaca gacaacctga ccgatgccat
     2161 caataagaga cacgccgaga tctacaaggg cctgttcaag gccgagctgt ttaatggcaa
     2221 ggtgctgaag cagctgggca ccgtgaccac aaccgagcac gagaacgccc tgctgcggag
     2281 cttcgacaag tttacaacct acttctccgg cttttatgag aacaggaaga acgtgttcag
     2341 cgccgaggat atcagcacag ccatcccaca ccgcatcgtg caggacaact tccccaagtt
     2401 taaggagaat tgtcacatct tcacacgcct gatcaccgcc gtgcccagcc tgcgggagca
     2461 ctttgagaac gtgaagaagg ccatcggcat cttcgtgagc acctccatcg aggaggtgtt
     2521 ttccttccct ttttataacc agctgctgac acagacccag atcgacctgt ataaccagct
     2581 gctgggagga atctctcggg aggcaggcac cgagaagatc aagggcctga acgaggtgct
     2641 gaatctggcc atccagaaga atgatgagac agcccacatc atcgcctccc tgccacacag
     2701 attcatcccc ctgtttaagc agatcctgtc cgataggaac accctgtctt tcatcctgga
     2761 ggagtttaag agcgacgagg aagtgatcca gtccttctgc aagtacaaga cactgctgag
     2821 aaacgagaac gtgctggaga cagccgaggc cctgtttaac gagctgaaca gcatcgacct
     2881 gacacacatc ttcatcagcc acaagaagct ggagacaatc agcagcgccc tgtgcgacca
     2941 ctgggataca ctgaggaatg ccctgtatga gcggagaatc tccgagctga caggcaagat
     3001 caccaagtct gccaaggaga aggtgcagcg cagcctgaag cacgaggata tcaacctgca
     3061 ggagatcatc tctgccgcag gcaaggagct gagcgaggcc ttcaagcaga aaaccagcga
     3121 gatcctgtcc cacgcacacg ccgccctgga tcagccactg cctacaaccc tgaagaagca
     3181 ggaggagaag gagatcctga agtctcagct ggacagcctg ctgggcctgt accacctgct
     3241 ggactggttt gccgtggatg agtccaacga ggtggacccc gagttctctg cccggctgac
     3301 cggcatcaag ctggagatgg agccttctct gagcttctac aacaaggcca gaaattatgc
     3361 caccaagaag ccctactccg tggagaagtt caagctgaac tttcagatgc ctacactggc
     3421 ctctggctgg gacgtgaata aggagaagaa caatggcgcc atcctgtttg tgaagaacgg
     3481 cctgtactat ctgggcatca tgccaaagca gaagggcagg tataaggccc tgagcttcga
     3541 gcccacagag aaaaccagcg agggctttga taagatgtac tatgactact tccctgatgc
     3601 cgccaagatg atcccaaagt gcagcaccca gctgaaggcc gtgacagccc actttcagac
     3661 ccacacaacc cccatcctgc tgtccaacaa tttcatcgag cctctggaga tcacaaagga
     3721 gatctacgac ctgaacaatc ctgagaagga gccaaagaag tttcagacag cctacgccaa
     3781 gaaaaccggc gaccagaagg gctacagaga ggccctgtgc aagtggatcg acttcacaag
     3841 ggattttctg tccaagtata ccaagacaac ctctatcgat ctgtctagcc tgcggccatc
     3901 ctctcagtat aaggacctgg gcgagtacta tgccgagctg aatcccctgc tgtaccacat
     3961 cagcttccag agaatcgccg agaaggagat catggatgcc gtggagacag gcaagctgta
     4021 cctgttccag atctataaca aggactttgc caagggccac cacggcaagc ctaatctgca
     4081 cacactgtat tggaccggcc tgttttctcc agagaacctg gccaagacaa gcatcaagct
     4141 gaatggccag gccgagctgt tctaccgccc taagtccagg atgaagagga tggcacaccg
     4201 gctgggagag aagatgctga acaagaagct gaaggatcag aaaaccccaa tccccgacac
     4261 cctgtaccag gagctgtacg actatgtgaa tcacagactg tcccacgacc tgtctgatga
     4321 ggccagggcc ctgctgccca acgtgatcac caaggaggtg tctcacgaga tcatcaagga
     4381 taggcgcttt accagcgaca agttcttttt ccacgtgcct atcacactga actatcaggc
     4441 cgccaattcc ccatctaagt tcaaccagag ggtgaatgcc tacctgaagg agcaccccga
     4501 gacacctatc atcggcatcg atcggggcga gagaaacctg atctatatca cagtgatcga
     4561 ctccaccggc aagatcctgg agcagcggag cctgaacacc atccagcagt ttgattacca
     4621 gaagaagctg gacaacaggg agaaggagag ggtggcagca aggcaggcct ggtctgtggt
     4681 gggcacaatc aaggatctga agcagggcta tctgagccag gtcatccacg agatcgtgga
     4741 cctgatgatc cactaccagg ccgtggtggt gctggagaac ctgaatttcg gctttaagag
     4801 caagaggacc ggcatcgccg agaaggccgt gtaccagcag ttcgagaaga tgctgatcga
     4861 taagctgaat tgcctggtgc tgaaggacta tccagcagag aaagtgggag gcgtgctgaa
     4921 cccataccag ctgacagacc agttcacctc ctttgccaag atgggcaccc agtctggctt
     4981 cctgttttac gtgcctgccc catatacatc taagatcgat cccctgaccg gcttcgtgga
     5041 ccccttcgtg tggaaaacca tcaagaatca cgagagccgc aagcacttcc tggagggctt
     5101 cgactttctg cactacgacg tgaaaaccgg cgacttcatc ctgcacttta agatgaacag
     5161 aaatctgtcc ttccagaggg gcctgcccgg ctttatgcct gcatgggata tcgtgttcga
     5221 gaagaacgag acacagtttg acgccaaggg cacccctttc atcgccggca agagaatcgt
     5281 gccagtgatc gagaatcaca gattcaccgg cagataccgg gacctgtatc ctgccaacga
     5341 gctgatcgcc ctgctggagg agaagggcat cgtgttcagg gatggctcca acatcctgcc
     5401 aaagctgctg gagaatgacg attctcacgc catcgacacc atggtggccc tgatccgcag
     5461 cgtgctgcag atgcggaact ccaatgccgc cacaggcgag gactatatca acagccccgt
     5521 gcgcgatctg aatggcgtgt gcttcgactc ccggtttcag aacccagagt ggcccatgga
     5581 cgccgatgcc aatggcgcct accacatcgc cctgaagggc cagctgctgc tgaatcacct
     5641 gaaggagagc aaggatctga agctgcagaa cggcatctcc aatcaggact ggctggccta
     5701 catccaggag ctgcgcaacg ctagcccaaa gaagaagagg aaagtctcta gataccctta
     5761 tgatgttcca gattatgccg gatacccata cgatgtccct gactatgcag gctcctaccc
     5821 ttatgacgtc ccagactacg ccggatccag gtccggcggc ggagagggca gaggaagtct
     5881 tctaacatgc ggtgacgtgg aggagaatcc cggcccaatg gtgagcaagg gcgaggagct
     5941 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     6001 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     6061 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     6121 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     6181 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     6241 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     6301 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     6361 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     6421 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     6481 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     6541 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     6601 cgggatcact ctcggcatgg acgagctgta caagtaactg cagcgcgggg atctcatgct
     6661 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     6721 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6781 caaactcatc aatgtatctt attaattaac gtcgacctct agctagagct tggcgtaatc
     6841 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6901 agccggaagc ataaagtgta aagcctaggg tgcctaatga gtgagctaac tcacattaat
     6961 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7021 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7081 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7141 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7201 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7261 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7321 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7381 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7441 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7501 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7561 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7621 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7681 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7741 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7801 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7861 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7921 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7981 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8041 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8101 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8161 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8221 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8281 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8341 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8401 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8461 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8521 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8581 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8641 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8701 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8761 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8821 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8881 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8941 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9001 cgacggatcg ggagatcgat ctcccgatcc cctagggtcg actctcagta caatctgctc
     9061 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     9121 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     9181 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat at
//
