LOCUS       sequence-240589-        9097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression, Genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9097)
  AUTHORS   Teng F, Li J, Cui T, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou
            Q, Li W
  TITLE     Enhanced mammalian genome editing by new Cas12a orthologs with 
            optimized crRNA scaffolds.
  JOURNAL   Genome Biol. 2019 Feb 5;20(1):15. doi: 10.1186/s13059-019-1620-8.
  PUBMED    30717767
REFERENCE   2  (bases 1 to 9097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2019-02-5"; volume: "20(1):15. doi"; pages: " 
            10.1186/s13059-019-1620-8"
COMMENT     Sequence Label: pCAG-ArCas12a-2AeGFP
FEATURES             Location/Qualifiers
     source          1..9097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          665..1681
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1605..1627
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1689..1708
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1742..1760
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1772..1861
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             1868..1888
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5687..5707
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5729..5782
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5783..5785,5786..5788,5789..6502)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5783..5785,5786..5788,5789..6499)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5828..5849)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6089..6108)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6436..6457
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6545..6666
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6582..6601)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6636..6655
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6709..6725)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6709..6725)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6722..6744)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6733..6749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6757..6763,6764..6781,6782..6787))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6802..6823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6940..6957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7111..7699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7191..7210)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7870..8661,8662..8730))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8493..8512
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8731..8835)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8914..8933)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
      421 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
      481 gcagcgatgg gggcgggggg ggggggggsg cgcgccrggs ggggsggggs ggggsgrggg
      541 gsggggsggg gsgrggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      841 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
      901 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1021 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1081 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1141 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1201 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1261 ggcggggccg cctcgggccg gggrgggctc gggggagggg cgcggcggcc cccggagcgc
     1321 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1381 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1441 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1501 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1561 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagcg cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag ctagtgaatt
     1741 ctaatacgac tcactatagg ccgccaccat gtacccttat gatgttccag attatgccgg
     1801 atacccatac gatgtccctg actatgcagg ctcctaccct tatgacgtcc cagactacgc
     1861 caccggtccc aagaagaaga ggaaggttcc cgggaacaac ggcacaaaca atttccagaa
     1921 cttcattggc atttcatcac tccagaagac cctgaggaat gccctgacac ctaccgagac
     1981 aacccagcaa ttcattgtga agaacggaat cattaaggag gacgaactga gaggcgagaa
     2041 ccgccagatc ctgaaagaca tcatggacga ctactatcgc ggtttcatct ccgagacact
     2101 cagcagcatt gatgatattg attggacatc tctgtttgag aagatggaaa tccagctgaa
     2161 gaatggcgac aacaaggaca ctctcattaa ggagcaagca gagaagagaa aggctatcta
     2221 caagaagttt gcagacgacg atcgcttcaa gaatatgttt agcgcaaagc tgataagtga
     2281 tattctccca gagtttgtga ttcacaacaa caactattct gccagcgaga aggaagagaa
     2341 gacacaggtc atcaagctct tctctcggtt tgccacctca ttcaaagatt acttcaagaa
     2401 tcgggcaaat tgtttctctg ccgatgacat ctcatcttcc tcctgtcaca gaatcgttaa
     2461 tgataacgcc gaaatcttct tctcaaatgc cctggtgtac cggagaatcg tgaagaatct
     2521 gagtaacgac gacatcaata agattagcgg tgatatgaag gactctctga agaagatgtc
     2581 cctggagaag atatacagct atgagaagta cggcgagttc atcactcaag agggaattag
     2641 cttctacaac gacatttgtg gcaaggttaa ctcattcatg aatctctact gccagaagaa
     2701 caaggagaat aagaatctgt acaaactgcg caaactgcat aagcaaatcc tgtgtatcgc
     2761 cgacacctct tatgaggtgc cctacaagtt tgagtctgac gaagaggtgt accagtccgt
     2821 gaacggattc ctggacaaca tcagttctaa acacatcgtc gagcggctga ggaagatcgg
     2881 cgacaactac aatggctata acctggacaa gatatacatc gtcagtaagt tctatgaaag
     2941 tgtgagtcag aagacctacc gggactggga gactatcaac accgcactcg aaatccacta
     3001 caataacatc ctgcctggca acggtaagag caaggccgac aaggtgaaga aggccgtcaa
     3061 gaacgacctc cagaagagca tcaccgagat taacgaactg gtgagtaact acaagctctg
     3121 cccagacgat aacattaagg cagaaaccta cattcatgag atttcccata tactgaacaa
     3181 ctttgaagct caagagctga aatacaatcc cgagattcat ctggtggaga gcgagctgaa
     3241 agcatccgag ctgaagaacg ttctcgacgt catcatgaac gccttccact ggtgtagcgt
     3301 gttcatgact gaggagctgg tcgataaaga caacaatttc tacgccgagc tggaagaaat
     3361 ctacgacgag atataccctg tgattagcct ctacaatctg gtccggaact atgtgaccca
     3421 gaagccctat tcaactaaga agatcaagct gaactttggg attcctaccc tggccgacgg
     3481 ctggtccaag agcaaagagt attccaataa cgcaatcatc ctgatgagag acaacctgta
     3541 ttacctcggt atctttaacg ctaagaataa gcccgagaag aagatcatcg aaggaaatac
     3601 atccgagaac aagggcgact acaagaagat gatctataac ctgctgcctg gcccaaacaa
     3661 gatgatccct aaggtgttcc tgagcagcaa gaccggagtc gagacttaca agccaagtgc
     3721 ctacatactg gagggctata agcagaacaa gcacctgaaa tctagcaaag atttcgacat
     3781 cactttctgt cgcgacctga tcgactattt caagaattgt attgccatcc acccagagtg
     3841 gaagaatttc ggattcgact tctctgacac ctccacatac gaggacatca gtggcttcta
     3901 tagagaagtg gagctgcaag gttacaagat cgactggacc tacatatctg agaaagacat
     3961 cgacctgctg caagagaaag ggcagctcta cctcttccaa atctacaaca aggactttag
     4021 taagaagtct acaggtaatg acaatctgca cactatgtac ctgaagaatc tcttctctga
     4081 agagaacctc aaagacgtgg tgctgaaact gaacggcgaa gcagaaatct tctttcgcaa
     4141 atcatccatt aagaatccta tcatacacaa gaagggtagt atcctggtga acaggacata
     4201 cgaagccgag gagaaagatc agttcggcaa cattcagatt gtgcgcaaga ctattcccga
     4261 gaatatctac caggagctgt acaaatactt caacgataag tctgataaag agctgtcaga
     4321 cgaagcagcc aagctgaaga atgctgtggg acatcatgaa gcagctacta acatcgtgaa
     4381 agactataga tacacatacg acaagtattt cctgcacatg ccaattacca tcaacttcaa
     4441 agccaataag acttctttca ttaacgaccg catcctccag tacattgcaa aggagaaaga
     4501 cctgcacgtg atcgggattg atcgcggaga acggaacctc atctacgttt cagtcatcga
     4561 cacatgcggt aacatcgtcg aacagaagag cttcaacatt gttaatgggt atgattacca
     4621 gatcaaactc aagcaacagg aaggcgcacg gcagattgct cgcaaggagt ggaaggaaat
     4681 tggcaagatc aaggaaatca aggaaggata cctcagcctc gtcattcatg aaatcagcaa
     4741 gatggtgatc aagtataacg caatcatcgc tatggaggac ctgagttatg gcttcaagaa
     4801 aggcagattc aaggtggagc ggcaagtcta ccagaaattc gaaacaatgc tgatcaacaa
     4861 gctgaactac ctggtgttca aagacatcag cataaccgag aacggaggac tcctgaaagg
     4921 ctaccagctc acatacatcc cagagaaact caagaatgtg ggccaccaat gcggctgcat
     4981 cttctacgtc cctgccgctt acaccagcaa gatagatccc actacaggat tcgtgaacat
     5041 attcaaattc aaggatctga cagtggacgc caagagggag ttcatcaaga agtttgatag
     5101 tattcgctat gacagcgata agaatctgtt ctgtttcacc tttgactaca acaacttcat
     5161 tacccagaat accgttatgt ccaagtctag ctggagtgtg tatacctacg gtgttcggat
     5221 caagcggagg tttgtcaatg gtagattctc aaacgaaagc gacaccatcg acatcacaaa
     5281 ggacatggag aagacactgg aaatgactga cataaactgg agagatggac acgacctgcg
     5341 gcaagacatc attgactacg agatcgttca gcacatcttt gaaatcttca agctgactgt
     5401 tcagatgcgg aatagtctga gcgagctgga ggaccggaat tacgaccgcc tgatctcacc
     5461 agtcctgaac gagaataaca tcttctacga ttctgccaaa gcaggagatg ccctgccaaa
     5521 ggacgctgat gcaaacggtg cctactgcat cgccctcaaa ggtctgtacg aaatcaagca
     5581 gattaccgag aattggaagg aggacgggaa gttcagcaga gacaagctca agatcagcaa
     5641 taaggactgg ttcgatttca ttcagaacaa gcgctacctg actagcccaa agaagaagag
     5701 gaaagtctct agaaggtccg gcggcggaga gggcagagga agtcttctaa catgcggtga
     5761 cgtggaggag aatcccggcc caatggtgag caagggcgag gagctgttca ccggggtggt
     5821 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     5881 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     5941 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     6001 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     6061 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     6121 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     6181 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     6241 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     6301 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     6361 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     6421 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     6481 catggacgag ctgtacaagt aactgcagcg cggggatctc atgctggagt tcttcgccca
     6541 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6601 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6661 atcttattaa ttaacgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     6721 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6781 gtgtaaagcc tagggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6841 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6901 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6961 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7021 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7081 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7141 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7201 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7261 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7321 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7381 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7441 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7501 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7561 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7621 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7681 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7741 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7801 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7861 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7921 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7981 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8041 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8101 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8161 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8221 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8281 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8341 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8401 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8461 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8521 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8581 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8641 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8701 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8761 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8821 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     8881 tcgatctccc gatcccctag ggtcgactct cagtacaatc tgctctgatg ccgcatagtt
     8941 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     9001 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     9061 gcgttttgcg ctgcttcgcg atgtacgggc cagatat
//
