LOCUS       sequence-240591-        8950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression, Genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Teng F, Li J, Cui T, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou
            Q, Li W
  TITLE     Enhanced mammalian genome editing by new Cas12a orthologs with 
            optimized crRNA scaffolds.
  JOURNAL   Genome Biol. 2019 Feb 5;20(1):15. doi: 10.1186/s13059-019-1620-8.
  PUBMED    30717767
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2019-02-5"; volume: "20(1):15. doi"; pages: " 
            10.1186/s13059-019-1620-8"
COMMENT     Sequence Label: pCAG-PrCas12a-2AeGFP
FEATURES             Location/Qualifiers
     source          1..8950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          665..1681
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1605..1627
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1689..1708
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1742..1760
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1772..1792
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5444..5464
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5471..5560
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             5582..5635
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5636..5638,5639..5641,5642..6355)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5636..5638,5639..5641,5642..6352)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5681..5702)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5942..5961)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6289..6310
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6398..6519
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6435..6454)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6489..6508
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6562..6578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6562..6578)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6575..6597)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6586..6602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6610..6616,6617..6634,6635..6640))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6655..6676
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6793..6810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6964..7552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7044..7063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7723..8514,8515..8583))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8346..8365
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8584..8688)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8767..8786)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
      421 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
      481 gcagcgatgg gggcgggggg ggggggggsg cgcgccrggs ggggsggggs ggggsgrggg
      541 gsggggsggg gsgrggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      841 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
      901 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1021 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1081 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1141 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1201 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1261 ggcggggccg cctcgggccg gggrgggctc gggggagggg cgcggcggcc cccggagcgc
     1321 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1381 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1441 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1501 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1561 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagcg cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag ctagtgaatt
     1741 ctaatacgac tcactatagg ccgccaccat gcccaagaag aagaggaagg ttcccgggat
     1801 gatcatcggc cgcgacttca acatgtacta ccagaacctg accaagatgt accccatcag
     1861 caagaccctg cgcaacgagc tgatccccgt gggcaagacc ctggagaaca tccgcaagaa
     1921 cggcatcctg gaggccgaca tccagcgcaa ggccgactac gagcacgtga agaagctgat
     1981 ggacaactac cacaagcagc tgatcaacga ggccctgcag ggcgtgcacc tgagcgacct
     2041 gagcgacgcc tacgacctgt acttcaacct gagcaaggag aagaacagcg tggacgcctt
     2101 cagcaagtgc caggacaagc tgcgcaagga gatcgtgagc ctgctgaaga accacgagaa
     2161 cttccccaag atcggcaaca aggagatcat caagctgctg cagagcctgt acgacaacga
     2221 caccgactac aaggccctgg acagcttcag caacttctac acctacttca gcagctacaa
     2281 cgaggtgcgc aagaacctgt acagcgacga ggagaagagc agcaccgtgg cctaccgcct
     2341 gatcaacgag aacctgccca agttcctgga caacatcaag gcctacgcca tcgccaagaa
     2401 ggccggcgtg cgcgccgagg gcctgagcga ggaggaccag gactgcctgt tcatcatcga
     2461 gaccttcgag cgcaccctga cccaggacgg catcgacaac tacaacgccg ccatcggcaa
     2521 gctgaacacc gccatcaacc tgttcaacca gcagaacaag aagcaggagg gcttccgcaa
     2581 ggtgccccag atgaagtgcc tgtacaagca gatcctgagc gaccgcgagg aggccttcat
     2641 cgacgagttc agcgacgacg aggacctgat caccaacatc gagagcttcg ccgagaacat
     2701 gaacgtgttc ctgaacagcg agatcatcac cgacttcaag atcgccctgg tggagagcga
     2761 cggcagcctg gtgtacatca agaacgacgt gagcaagacc agcttcagca acatcgtgtt
     2821 cggcagctgg aacgccatcg acgagaagct gagcgacgag tacgacctgg ccaacagcaa
     2881 gaagaagaag gacgagaagt actacgagaa gcgccagaag gagctgaaga agaacaagag
     2941 ctacgacctg gagaccatca tcggcctgtt cgacgacaac agcgacgtga tcggcaagta
     3001 catcgagaag ctggagagcg acatcaccgc catcgccgag gccaagaacg acttcgacga
     3061 gatcgtgctg cgcaagcacg acaagaacaa gagcctgcgc aagaacacca acgccgtgga
     3121 ggccatcaag agctacctgg acaccgtgaa ggacttcgag cgcgacatca agctgatcaa
     3181 cggcagcggc caggaggtgg agaagaacct ggtggtgtac gccgagcagg agaacatcct
     3241 ggccgagatc aagaacgtgg acagcctgta caacatgagc cgcaactacc tgacccagaa
     3301 gcccttcagc accgagaagt tcaagctgaa cttcaaccgc gccaccctgc tgaacggctg
     3361 ggacaagaac aaggagaccg acaacctggg catcctgttc gagaaggacg gcatgtacta
     3421 cctgggcatc atgaacacca aggccaacaa gatcttcgtg aacatcccca aggccaccag
     3481 caacgacgtg taccacaagg tgaactacaa gctgctgccc ggccccaaca agatgctgcc
     3541 caaggtgttc ttcgcccaga gcaacctgga ctactacaag cccagcgagg agctgctggc
     3601 caagtacaag gccggcaccc acaagaaggg cgacaacttc agcctggagg actgccacgc
     3661 cctgatcgac ttcttcaagg ccagcatcga gaagcacccc gactggagca gcttcggctt
     3721 cgagttcagc gagacctgca cctacgagga cctgagcggc ttctaccgcg aggtggagaa
     3781 gcagggctac aagatcacct acaccgacgt ggacgccgac tacatcacca gcctggtgga
     3841 gcgcgacgag ctgtacctgt tccagatcta caacaaggac ttcagcccct acagcaaggg
     3901 caacctgaac ctgcacacca tctacctgca gatgctgttc gaccagcgca acctgaacaa
     3961 cgtggtgtac aagctgaacg gcgaggccga ggtgttctac cgccccgcca gcatcaacga
     4021 cgaggaggtg atcatccaca aggccggcga ggagatcaag aacaagaaca gcaagcgcgc
     4081 cgtggacaag cccaccagca agttcggcta cgacatcatc aaggaccgcc gctacagcaa
     4141 ggacaagttc atgctgcaca tccccgtgac catgaacttc ggcgtggacg agacccgccg
     4201 cttcaacgac gtggtgaacg acgccctgcg caacgacgag aaggtgcgcg tgatcggcat
     4261 cgaccgcggc gagcgcaacc tgctgtacgt ggtggtggtg gacaccgacg gcaccatcct
     4321 ggagcagatc agcctgaaca gcatcatcaa caacgagtac agcatcgaga ccgactacca
     4381 caagctgctg gacgagaagg agggcgaccg cgaccgcgcc cgcaagaact ggaccaccat
     4441 cgagaacatc aaggagctga aggagggcta cctgagccag gtggtgaacg tgatcgccaa
     4501 gctggtgctg aagtacaacg ccatcatctg cctggaggac ctgaacttcg gcttcaagcg
     4561 cggccgccag aaggtggaga agcaggtgta ccagaagttc gagaagatgc tgatcgacaa
     4621 gctgaactac ctggtgatcg acaagagccg caagcaggac aagcccgagg agttcggcgg
     4681 cgccctgaac gccctgcagc tgaccagcaa gttcaccagc ttcaaggaca tgggcaagca
     4741 gaccggcatc atctactacg tgcccgccta cctgaccagc aagatcgacc ccaccaccgg
     4801 cttcgccaac ctgttctacg tgaagtacga gaacgtggag aaggccaagg agttcttcag
     4861 ccgcttcgac agcatcagct acaacaacga gagcggctac ttcgagttcg ccttcgacta
     4921 caagaagttc accgaccgcg cctgcggcgc ccgcagccag tggaccgtgt gcacctacgg
     4981 cgagcgcatc atcaagttcc gcaacaccga gaagaacaac agcttcgacg acaagaccat
     5041 cgtgctgagc gaggagttca aggagctgtt cagcatctac ggcatcagct acgaggacgg
     5101 cgccgagctg aagaacaaga tcatgagcgt ggacgaggcc gacttcttcc gcagcctgac
     5161 ccgcctgttc cagcagacca tgcagatgcg caacagcagc aacgacgtga cccgcgacta
     5221 catcatcagc cccatcatga acgaccgcgg cgagttcttc aacagcgagg cctgcgacgc
     5281 cagcaagccc aaggacgccg acgccaacgg cgccttcaac atcgcccgca agggcctgtg
     5341 ggtgctggag cagatccgca acacccccag cggcgacaag ctgaacctgg ccatgagcaa
     5401 cgccgagtgg ctggagtacg cccagcgcaa ccagatcgct agcccaaaga agaagaggaa
     5461 agtctctaga tacccttatg atgttccaga ttatgccgga tacccatacg atgtccctga
     5521 ctatgcaggc tcctaccctt atgacgtccc agactacgcc ggatccaggt ccggcggcgg
     5581 agagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gcccaatggt
     5641 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     5701 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     5761 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     5821 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     5881 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     5941 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     6001 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     6061 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     6121 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     6181 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     6241 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     6301 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaactgca
     6361 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     6421 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6481 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat taattaacgt cgacctctag
     6541 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6601 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctagggtg cctaatgagt
     6661 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6721 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6781 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6841 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6901 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6961 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7081 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7141 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7201 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7261 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7321 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7381 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7441 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7501 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7561 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7621 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7681 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7741 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7801 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7861 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7921 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7981 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8041 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8101 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8161 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8221 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8281 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8341 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8401 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8461 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8521 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8581 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8641 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8701 aaaagtgcca cctgacgtcg acggatcggg agatcgatct cccgatcccc tagggtcgac
     8761 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     8821 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     8881 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     8941 ggccagatat
//
