LOCUS       sequence-240595-        5893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the fluorescent protein mNeonGreen in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5893)
  TITLE     AAV expression plasmids for production of mNeonGreen
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-UbC-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..5893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..421
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     991..1010
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     1707..1726
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1749..1758
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2010..2019
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2510..2986
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2709..2728)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3026..3166
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3026..3155
                     /label=AAV2 ITR
     rep_origin      3241..3696
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3328..3347)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3538..3559
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3713..3732)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3832..3854
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3892..3910)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3978..4082
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4083..4151,4152..4943)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4301..4320)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5114..5702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5603..5622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   5764..5893
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gcggccgcac gcgtttaaac tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc
       61 ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt
      121 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt
      181 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc
      241 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc
      301 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
      361 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
      421 tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac
      481 ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg
      541 aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatagaacc
      601 ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc
      661 ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca
      721 gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg
      781 tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg
      841 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga
      901 atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt
      961 cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg
     1021 ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt
     1081 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
     1141 cttttttgtt agacgaagct aaggcgcgcc tgagaacttc agggtgagtt tggggaccct
     1201 tgattgttct ttctttttcg ctattgtaaa attcatgtta tatggagggg gcaaagtttt
     1261 cagggtgttg tttagaatgg gaagatgtcc cttgtatcac catgcatgga ccctcatgat
     1321 aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt
     1381 cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatc tagacaatca
     1441 gggtatatta tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata
     1501 aggtagaata tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa
     1561 caactacatc ctggtcatca tcctgccttt ctctttatgg ttacaatgat atacactgtt
     1621 tgagatgagg ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc
     1681 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcaagc
     1741 ttggatccgc caccatggtt tccaagggcg aagaggacaa tatggccagc ctgcctgcca
     1801 cacacgagct gcacatcttt ggcagcatca acggcgtgga cttcgacatg gttggacaag
     1861 gcaccggcaa tcccaacgac ggctacgagg aactgaacct gaagtccaca aagggcgacc
     1921 tgcagttcag cccttggatt ctggtgcctc acatcggcta cggcttccac cagtacctgc
     1981 cttatcctga cggcatgagc ccttttcagg ccgccatggt ggatggctct ggctaccagg
     2041 ttcaccggac catgcagttt gaagatggcg ccagcctgac cgtgaactac agatacacat
     2101 acgagggcag ccacatcaaa ggcgaggccc aagtgaaagg cacaggcttt ccagccgatg
     2161 gacccgtgat gaccaatagc ctgacagccg ccgactggtg cagaagcaag aaaacatacc
     2221 ccaacgacaa gaccatcatc agcaccttca agtggtccta caccaccggc aacggcaaga
     2281 gatacagaag caccgccaga accacctaca ccttcgccaa acctatggcc gccaactacc
     2341 tgaagaacca gcctatgtac gtgttccgca agaccgagct gaagcacagc aagacagaac
     2401 tgaacttcaa agagtggcag aaagccttca ccgacgtgat gggcatggac gagctgtata
     2461 agtaggctag cctcgagtct agagttaacg gccatattgg ccaatctacg ggtggcatcc
     2521 ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag
     2581 ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat
     2641 tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc
     2701 ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat
     2761 ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca
     2821 ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat
     2881 attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa
     2941 attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttgtaggt
     3001 aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct
     3061 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     3121 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
     3181 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     3241 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3301 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3361 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     3421 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     3481 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     3541 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
     3601 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
     3661 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     3721 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     3781 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     3841 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     3901 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     3961 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4021 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4081 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4141 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4201 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4261 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4321 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4381 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4441 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4501 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4561 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4621 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4681 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4741 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4801 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4861 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4921 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4981 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5041 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5101 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5161 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5221 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5281 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5341 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5401 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5461 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5521 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5581 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5641 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5701 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5761 tgtcctgcag gcagctgcgc gctcgctcgc tcactgaggc cgcccgggcg tcgggcgacc
     5821 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
     5881 actaggggtt cct
//
