LOCUS       sequence-240610-       10687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance 
            gene under control of TetON promoter. This 3rd generation lentiviral
            vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Ho SM, Hartley BJ, Tcw J, Beaumont M, Stafford K, Slesinger PA, 
            Brennand KJ
  TITLE     Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural
            progenitor cells.
  JOURNAL   Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. doi: 
            10.1016/j.ymeth.2015.11.019.
  PUBMED    26626326
REFERENCE   2  (bases 1 to 10687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Nov 25. pii: S1046-2023(15)30159-6. doi: 
            10.1016/j.ymeth.2015.11.019."
COMMENT     Sequence Label: pLV-TetO-hNGN2-eGFP-Puro
FEATURES             Location/Qualifiers
     source          1..10687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2377..2395,2396..2418,2419..2437,2438..2460,2461..2479,
                     2480..2502,2503..2521,2522..2544,2545..2563,2564..2586,
                     2587..2605,2606..2628,2629..2647)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2680..2718
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2715..2739
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3655..3711
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3724..4434
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             3724..4434
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(3763..3784)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4024..4043)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4371..4392
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4444..4497
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4510..5103
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="EYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVT
                     ELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQ
                     QQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAP
                     RNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5000..5020
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5142..5730
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5195..5215)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5613..5624)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5733..5749)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5734..5750)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6255..6435
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6461..6478)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6467..6691
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6737..7165
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6824..6843)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7034..7055
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7174..7194)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7179..7508
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7359..7494
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7421..7440
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7556..7603
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7622..7996
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8126..8247
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8163..8182)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8217..8236
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8296..8312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8296..8312)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8309..8331)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8320..8336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8344..8350,8351..8368,8369..8374))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8389..8410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8527..8544)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8698..9286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8778..8797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9457..10248,10249..10317))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10080..10099
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10318..10422)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10497..10516)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttag atctcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2581 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2641 atagagaaaa gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt acggtgggag
     2701 gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     2761 tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc cgaattcgcc
     2821 accatgttcg tcaaatccga gaccttggag ttgaaggagg aagaggacgt gttagtgctg
     2881 ctcggatcgg cctcccccgc cttggcggcc ctgaccccgc tgtcatccag cgccgacgaa
     2941 gaagaggagg aggagccggg cgcgtcaggc ggggcgcgtc ggcagcgcgg ggctgaggcc
     3001 gggcaggggg cgcggggcgg cgtggctgcg ggtgcggagg gctgccggcc cgcacggctg
     3061 ctgggtctgg tacacgattg caaacggcgc ccttcccggg cgcgggccgt ctcccgaggc
     3121 gccaagacgg ccgagacggt gcagcgcatc aagaagaccc gtagactgaa ggccaacaac
     3181 cgcgagcgaa accgcatgca caacctcaac gcggcactgg acgcgctgcg cgaggtgctc
     3241 cccacgttcc ccgaggacgc caagctcacc aagatcgaga ccctgcgctt cgcccacaac
     3301 tacatctggg cactcaccga gaccctgcgc ctggcggatc actgcggggg cggcggcggg
     3361 ggcctgccgg gggcgctctt ctccgaggca gtgttgctga gcccgggagg agccagcgcc
     3421 gccctgagca gcagcggaga cagcccctcg cccgcctcca cgtggagttg caccaacagc
     3481 cccgcgccgt cctcctccgt gtcctccaat tccacctccc cctacagctg cactttatcg
     3541 cccgccagcc cggccgggtc agacatggac tattggcagc ccccacctcc cgacaagcac
     3601 cgctatgcac ctcacctccc catagccagg gattgtatcg aattcggatc tggcgccacg
     3661 aatttctcgc tactcaagca ggccggtgat gtcgaggaaa accctggtcc tatggtgtct
     3721 agaagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     3781 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     3841 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     3901 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     3961 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     4021 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     4081 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     4141 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     4201 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     4261 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     4321 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     4381 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggttca
     4441 ggggagggaa gaggttccct gctcacatgc ggagacgtag aagagaatcc cggacccatg
     4501 acctctagag agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     4561 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     4621 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     4681 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     4741 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     4801 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     4861 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     4921 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     4981 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     5041 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     5101 tgattaatta agctagctaa ttcgatatca agcttatcga taatcaacct ctggattaca
     5161 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5221 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5281 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5341 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5401 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5461 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5521 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     5581 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5641 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5701 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa
     5761 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     5821 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     5881 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5941 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     6001 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     6061 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     6121 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     6181 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     6241 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6301 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6361 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6421 ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     6481 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     6541 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     6601 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     6661 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     6721 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6781 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6841 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6901 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6961 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7021 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7081 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7141 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     7201 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7261 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7321 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7381 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7441 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7501 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     7561 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     7621 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     7681 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     7741 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     7801 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     7861 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     7921 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     7981 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     8041 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     8101 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     8161 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8221 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     8281 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8341 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8401 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8461 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8521 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8581 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8641 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8701 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8761 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8821 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8881 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8941 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9001 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9061 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9121 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9181 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9241 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9301 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9361 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9421 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9481 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9541 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9601 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9661 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9721 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9781 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9841 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9901 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9961 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10021 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10081 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10141 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10201 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10261 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10321 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10381 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10441 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
    10501 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
    10561 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
    10621 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
    10681 acgcgtt
//
