LOCUS       sequence-240615-         202 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene knockout/in for actinomycetes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 202)
  AUTHORS   Tong Y, Charusanti P, Zhang L, Weber T, Lee SY
  TITLE     CRISPR-Cas9 Based Engineering of Actinomycetal Genomes.
  JOURNAL   ACS Synth Biol. 2015 Sep 18;4(9):1020-9. doi: 
            10.1021/acssynbio.5b00038. Epub 2015 Apr 7.
  PUBMED    25806970
REFERENCE   2  (bases 1 to 202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00038"; journalName: "ACS Synth Biol"; date: 
            "2015-09-18- 18"; volume: "4"; issue: "9"; pages: "1020-9"
COMMENT     Sequence Label: pCRISPR-Cas9
FEATURES             Location/Qualifiers
     source          1..202
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        121..196
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gcggtcgatc ttgacggctg gcgagaggtg cggggaggat ctgaccgacg cggtccacac
       61 gtggcaccgc gatgctgttg tgggcacaat cgtgccggtt ggtaggatcg acggccatgg
      121 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
      181 ggcaccgagt cggtgctttt tt
//
