LOCUS       sequence-240617-         201 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C to T base editor for actinomycetes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 201)
  AUTHORS   Tong Y, Whitford CM, Robertsen HL, Blin K, Jorgensen TS, Klitgaard 
            AK, Gren T, Jiang X, Weber T, Lee SY
  TITLE     Highly efficient DSB-free base editing for streptomycetes with 
            CRISPR-BEST.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Oct 8;116(41):20366-20375. doi: 
            10.1073/pnas.1913493116. Epub 2019 Sep 23.
  PUBMED    31548381
REFERENCE   2  (bases 1 to 201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1913493116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-10-8- 8"; volume: "116"; issue: "41"; pages: 
            "20366-20375"
COMMENT     Sequence Label: pCRISPR-cBEST
FEATURES             Location/Qualifiers
     source          1..201
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        120..195
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gcggtcgatc ttgacggctg gcgagaggtg cggggaggat ctgaccgacg cggtccacac
       61 gtggcaccgc gatgctgttg tgggcacaat cgtgccggtt ggtaggatcg acggccatgg
      121 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
      181 gcaccgagtc ggtgcttttt t
//
