LOCUS       sequence-240625-        4597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  p300 core domain with GreenGate D-E flanking sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Lee JE, Neumann M, Duro DI, Schmid M
  TITLE     CRISPR-based tools for targeted transcriptional and epigenetic 
            regulation in plants.
  JOURNAL   PLoS One. 2019 Sep 26;14(9):e0222778. doi: 
            10.1371/journal.pone.0222778. eCollection 2019.
  PUBMED    31557222
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-09-26"; volume: "14(9):e0222778. doi"; pages: " 
            10.1371/journal.pone.0222778. eCollection 2019"
COMMENT     Sequence Label: pJL023
FEATURES             Location/Qualifiers
     source          1..4597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2376..2392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2376..2392)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2389..2411)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2400..2416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2424..2430,2431..2448,2449..2454))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2469..2490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2607..2624)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2778..3366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2858..2877)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3537..4328,4329..4397))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4160..4179
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4398..4502)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4570..4588
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tatcggatcc cgacggtctc atcaggtatt tttaagccag aggagcttag
      481 acaagcactt atgcctactc ttgaggctct ttacagacaa gatcctgaat ctcttccatt
      541 cagacagcct gtggaccctc agcttcttgg tattcctgac tacttcgata ttgtgaagtc
      601 tcctatggat ctttctacaa ttaaaagaaa acttgacaca ggacaatacc aagaaccttg
      661 gcaatacgtt gatgacatct ggcttatgtt taacaatgct tggctttaca acagaaagac
      721 ttctagagtt tacaaatatt gctctaaact ttctgaagtg ttcgaacaag aaattgatcc
      781 agtgatgcaa tctcttggtt actgttgcgg tagaaaactt gagttctctc ctcaaactct
      841 ttgctgctat ggaaagcaac tttgtactat tcctagagat gcaacatact attcttatca
      901 aaacagatac catttctgtg aaaaatgctt taacgaaatc cagggtgaat ctgtttctct
      961 tggagacgac ccttctcagc ctcagacaac aatcaataaa gagcagttct ctaagagaaa
     1021 gaacgatact cttgaccctg agcttttcgt ggagtgtact gagtgcggta gaaagatgca
     1081 tcagatttgt gttcttcatc acgagatcat ttggccagct ggttttgttt gcgatggttg
     1141 tcttaagaag tctgcaagaa ctagaaaaga gaataagttc tctgcaaaga gacttccttc
     1201 tactagactt ggaactttcc ttgaaaacag agtgaacgat ttccttagaa gacagaatca
     1261 tcctgagtct ggagaggtga cagtgagagt ggttcatgca tctgataaaa ctgttgaggt
     1321 taaacctgga atgaaagcta gattcgtgga ctctggagag atggcagaat cttttccata
     1381 cagaacaaaa gctctttttg cattcgaaga gattgatggt gttgatcttt gtttcttcgg
     1441 tatgcacgtg caagagtatg gatctgactg ccctccacca aatcaaagaa gagtttacat
     1501 ttcttatctt gattctgttc acttttttag accaaaatgc cttagaacag cagtgtacca
     1561 cgagattctt attggttatc ttgagtatgt gaaaaaactt ggttacacaa ctggacatat
     1621 ttgggcttgc ccaccttctg aaggtgatga ttatattttt cattgccacc ctccagacca
     1681 aaagattcct aaacctaaaa gacttcaaga gtggtacaaa aagatgcttg ataaagcagt
     1741 gtctgagaga attgtgcacg actacaagga catttttaaa caggctacag aggatagact
     1801 tacttctgca aaagagcttc cttattttga aggagatttt tggcctaatg ttcttgagga
     1861 gtctattaag gaacttgagc aggaggaaga agagagaaaa agagaagaga acacatctaa
     1921 tgagtctaca gacgttacta aaggtgattc taaaaacgct aaaaagaaaa acaataaaaa
     1981 aacttctaaa aataaatctt ctctttctag aggtaacaaa aaaaaacctg gaatgccaaa
     2041 cgtttctaat gacctttctc aaaaacttta cgcaacaatg gaaaaacata aagaagtttt
     2101 cttcgtgatc agacttattg ctggacctgc tgctaattct cttcctccaa tcgttgaccc
     2161 agaccctctt attccttgtg atcttatgga tggaagagac gcatttctta ctcttgcaag
     2221 agacaaacat cttgaatttt cttctcttag aagagcacag tggtctacaa tgtgtatgct
     2281 tgttgagctt cacacacaat ctcaagacta gtaactgcag agaccgacgg gcccgtcgac
     2341 tgcagaggcc tgcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     2401 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     2461 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     2521 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     2581 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2641 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2701 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2761 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2821 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2881 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2941 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3001 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3061 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3121 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3181 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3241 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3301 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3361 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3421 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3481 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3541 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3601 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3661 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3721 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3781 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3841 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3901 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3961 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4021 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4081 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4141 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4201 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4261 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4321 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4381 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4441 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4501 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     4561 acctataaaa ataggcgtat cacgaggccc tttcgtc
//
