LOCUS       sequence-240644-        1798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS491
FEATURES             Location/Qualifiers
     source          1..1798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(165..199)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(270..929)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     844..863
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1111..1699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1600..1619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc attgatatat aaattctctt gcattttcta tttttctctc tatctattct
       61 acttgtttat tcccttcaag gttttttttt aaggagtact tgtttttaga atatacggtc
      121 aacgaactat aattaactaa acagatctat gtgagaccag accaataaaa aacgcccggc
      181 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
      241 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac
      301 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
      361 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
      421 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
      481 cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
      541 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
      601 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
      661 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
      721 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
      781 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
      841 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
      901 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
      961 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     1021 acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1081 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1141 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1201 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1261 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1321 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1381 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1441 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1501 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1561 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1621 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1681 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1741 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgt
//
