LOCUS       sequence-24065-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  TITLE     T4 Lysozyme Plasmids - Tray 1
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme 7003A
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(707..729)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    718..734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gaaaagcccg ctcattaggc gggctcagat ctgctcatgt ttgacagctt atcatcgata
       61 agcttcggca ataaattctt gatatgtttt catatgtttc ctttaaatat tttaattagt
      121 aattgtctat tcaagtaatt gtgaatatac tatcacaatt ccaagagaaa gtaaacagct
      181 ttatagattt ttatacgcgt cccaagtgcc agttctaaac gttgtaatga ctcgttttgc
      241 gcgattaggt gtttgattat accatctact tttagctaag gcagctgctg ctgcatccca
      301 gcgtttttgt tgaagcatac gtaaagagtt agtaaatcct gccacaccgg tttctcccat
      361 ttggaaaacc atattaatca atgcacagcg acgaaccgca tcaagagaat cataaaccgg
      421 ttttaattta gcatttctca gaattccgcg aacagcagca tcaacatcct gattaaagag
      481 tttttcagcc tcatcttttg taattacacc attgcaatta cgcccaatag ctttatctaa
      541 ttcagattta gcagcattaa gtgatgcagc tgctgcaagc aaatgaccga tgccaatagt
      601 gtaatagcct tctgtgtctt tatagatttt aagtctaaga ccttcatcta tacgtaacat
      661 ttcaaatata ttcataatac ctcctaagca tcgatggatc ctgtttcctg tgtgaaattg
      721 ttatccgctc acaattccac acattatacg agccgatgat antgtcacag gggatgggag
      781 tagctgacct gttcnggnga atgtancgnc catccccata acagcgagat atgcacnggg
      841 atggagac
//
