LOCUS       sequence-240651-        1801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS493
FEATURES             Location/Qualifiers
     source          1..1801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(168..202)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(273..932)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     847..866
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1114..1702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1603..1622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc attgatatat aaatgtaata agaagtttgg taatattcaa ttcgaagtgt
       61 tcagtctttt acttctcttg ttttatagaa gaaaaaacat caagaaacat ctttaacata
      121 cacaaacaca tactatcaga atacaagatc tatgtgagac cagaccaata aaaaacgccc
      181 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
      241 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
      301 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
      361 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
      421 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
      481 acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc
      541 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
      601 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
      661 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc
      721 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
      781 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
      841 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
      901 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
      961 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     1021 cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1081 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1141 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1201 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1261 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1321 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1381 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1441 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1501 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1561 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1621 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1681 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     1741 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     1801 t
//
