LOCUS       sequence-240653-        1818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS486
FEATURES             Location/Qualifiers
     source          1..1818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     122..145
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     terminator      complement(185..219)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(290..949)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     864..883
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1131..1719
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1620..1639
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc attgatatat aaatggaaaa gctgcataac cactttaact aatactttca
       61 acattttcag tttgtattac ttcttattca aatgtcataa aagtatcaac aaaaaattgt
      121 taatatacct ctatacttta acgtcaagga gaaaaaacta taagatctat gtgagaccag
      181 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
      241 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
      301 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
      361 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
      421 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
      481 caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc
      541 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
      601 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
      661 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
      721 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
      781 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
      841 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
      901 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
      961 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     1021 ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag
     1081 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1141 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1201 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1261 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     1321 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1381 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1441 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1501 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1561 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1621 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1681 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1741 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1801 gattctgtgg ataaccgt
//
