LOCUS       sequence-240658-       11109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11109)
  AUTHORS   Lane MC, Alteri CJ, Smith SN, Mobley HL
  TITLE     Expression of flagella is coincident with uropathogenic Escherichia 
            coli ascension to the upper urinary tract.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16669-74. Epub 2007 
            Oct 9.
  PUBMED    17925449
REFERENCE   2  (bases 1 to 11109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-10-16"; volume: "104(42)"; pages: 
            "16669-74. Epub 2007 Oct 9"
COMMENT     Sequence Label: pGEN-luxCDABE
FEATURES             Location/Qualifiers
     source          1..11109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        887..934
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1021..2463
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             2476..3399
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             3448..4530
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             4545..5528
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             5707..6819
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     primer_bind     6923..6943
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     rep_origin      complement(7074..7619)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7154..7173)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(7783..7869)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     8022..8039
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(join(8584..9375,9376..9444))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9207..9226
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9445..9549)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9617..9635
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 taataactat ctgaagcaac gaattaatga tgagaacgaa atatcaatag tcaccgaagc
       61 aatgaatgaa gcacttcgta aacttgagca acgtgtatta aatacgctca atgaattttc
      121 tggttatact catgttatgg ttataggcgg tggcgcagaa ttaatatgcg atgcagtaaa
      181 aaaacacaca cagattcgtg atgaacgttt tttcaaaacc aataactctc aatatgattt
      241 agttaacggt atgtatctca taggtaatta atgatggaca agcgcagaac cattgccttc
      301 aaactaaatc cagatgtaaa tcaaacagat aaaattgttt gtgatacact ggacagtatc
      361 ccgcaagggg aacgaagccg ccttaaccgg gccgcactga cggcaggtct ggccttatac
      421 agacaagatc cccggacccc tttcctttta tgtgagctgc tgacgaaaga aaccacattt
      481 tcagatatcg tgaatatatt gagatcgcta tttccaaaag agatggccga ttttaattct
      541 tcaatagtca ctcaatcctc ttcacaacaa gagcaaaaaa gtgatgaaga gaccaaaaaa
      601 aatgcgatga agctaataaa ttaattcaat tattattgag ttccctttat ccactatcag
      661 gctggataaa gggaactcaa tcaagttatt ttcttaccag tcattacata atcgttatta
      721 tgaaataatc gtttgcactg tctctgttat tcaggcaatt tcaataaagg cacttgctca
      781 cgctctgtca ttttctgaaa ctctcatgtg gaattcctgc agggcatgcc ccgggcatat
      841 gaagcttggt accgggatcc gcggccgcgt ttaaacccat ggacgtgttg acaattaatc
      901 atcggcatag tatatcggca tagtataata cgactcaggg cccactagtg gtacccgggg
      961 atcctctaga gtcgacctgc aggtcgacgg atccggggaa ttcaggcttg gaggatacgt
     1021 atgactaaaa aaatttcatt cattattaac ggccaggttg aaatctttcc cgaaggtgat
     1081 gatttagtgc aatccattaa ttttggtgat aatagtgttt acctgccaat attgaatgac
     1141 tctcatgtaa aaaacattat tgattgtaat ggaaataacg aattacggtt gcataacatt
     1201 gtcaattttc tctatacggt agggcaaaga tggaaaaatg aagaatactc aagacgcagg
     1261 acatacattc gtgacttaaa aaaatatatg ggatattcag aagaaatggc taagctagag
     1321 gccaattgga tatctatgat tttatgttct aaaggcggcc tttatgatgt tgtagaaaat
     1381 gaacttggtt ctcgccatat catggatgaa tggctacctc aggatgaaag ttatgttcgg
     1441 gcttttccga aaggtaaatc tgtacatctg ttggcaggta atgttccatt atctgggatc
     1501 atgtctatat tacgcgcaat tttaactaag aatcagtgta ttataaaaac atcgtcaacc
     1561 gatcctttta ccgctaatgc attagcgtta agttttattg atgtagaccc taatcatccg
     1621 ataacgcgct ctttatctgt tatatattgg ccccaccaag gtgatacatc actcgcaaaa
     1681 gaaattatgc aacatgcgga tgttattgtc gcttggggag ggccagatgc gattaattgg
     1741 gcggtagagc atgcgccatc ttatgctgat gtgattaaat ttggttctaa aaagagtctt
     1801 tgcattatcg ataatcctgt tgatttgacg tccgcagcga caggtgcggc tcatgatgtt
     1861 tgtttttacg atcagcgagc ttgtttttct gcccaaaaca tatattacat gggaaatcat
     1921 tatgaggaat ttaagttagc gttgatagaa aaacttaatc tatatgcgca tatattaccg
     1981 aatgccaaaa aagattttga tgaaaaggcg gcctattctt tagttcaaaa agaaagcttg
     2041 tttgctggat taaaagtaga ggtggatatt catcaacgtt ggatgattat tgagtcaaat
     2101 gcaggtgtgg aatttaatca accacttggc agatgtgtgt accttcatca cgtcgataat
     2161 attgagcaaa tattgcctta tgttcaaaaa aataagacgc aaaccatatc tatttttcct
     2221 tgggagtcat catttaaata tcgagatgcg ttagcattaa aaggtgcgga aaggattgta
     2281 gaagcaggaa tgaataacat atttcgagtt ggtggatctc atgacggaat gagaccgttg
     2341 caacgattag tgacatatat ttctcatgaa aggccatcta actatacggc taaggatgtt
     2401 gcggttgaaa tagaacagac tcgattcctg gaagaagata agttccttgt atttgtccca
     2461 taataggtaa aaagtatgga aaatgaatca aaatataaaa ccatcgacca cgttatttgt
     2521 gttgaaggaa ataaaaaaat tcatgtttgg gaaacgctgc cagaagaaaa cagcccaaag
     2581 agaaagaatg ccattattat tgcgtctggt tttgcccgca ggatggatca ttttgctggt
     2641 ctggcggaat atttatcgcg gaatggattt catgtgatcc gctatgattc gcttcaccac
     2701 gttggattga gttcagggac aattgatgaa tttacaatgt ctataggaaa gcagagcttg
     2761 ttagcagtgg ttgattggtt aactacacga aaaataaata acttcggtat gttggcttca
     2821 agcttatctg cgcggatagc ttatgcaagc ctatctgaaa tcaatgcttc gtttttaatc
     2881 accgcagtcg gtgttgttaa cttaagatat tctcttgaaa gagctttagg gtttgattat
     2941 ctcagtctac ccattaatga attgccgaat aatctagatt ttgaaggcca taaattgggt
     3001 gctgaagtct ttgcgagaga ttgtcttgat tttggttggg aagatttagc ttctacaatt
     3061 aataacatga tgtatcttga tataccgttt attgctttta ctgcaaataa cgataattgg
     3121 gtcaagcaag atgaagttat cacattgtta tcaaatattc gtagtaatcg atgcaagata
     3181 tattctttgt taggaagttc gcatgacttg agtgaaaatt tagtggtcct gcgcaatttt
     3241 tatcaatcgg ttacgaaagc cgctatcgcg atggataatg atcatctgga tattgatgtt
     3301 gatattactg aaccgtcatt tgaacattta actattgcga cagtcaatga acgccgaatg
     3361 agaattgaga ttgaaaatca agcaatttct ctgtcttaaa atctattgag atattctatc
     3421 actcaaatag caatataagg actctctatg aaatttggaa actttttgct tacataccaa
     3481 cctccccaat tttctcaaac agaggtaatg aaacgtttgg ttaaattagg tcgcatctct
     3541 gaggagtgtg gttttgatac cgtatggtta ctggagcatc atttcacgga gtttggtttg
     3601 cttggtaacc cttatgtcgc tgctgcatat ttacttggcg cgactaaaaa attgaatgta
     3661 ggaactgccg ctattgttct tcccacagcc catccagtac gccaacttga agatgtgaat
     3721 ttattggatc aaatgtcaaa aggacgattt cggtttggta tttgccgagg gctttacaac
     3781 aaggactttc gcgtattcgg cacagatatg aataacagtc gcgccttagc ggaatgctgg
     3841 tacgggctga taaagaatgg catgacagag ggatatatgg aagctgataa tgaacatatc
     3901 aagttccata aggtaaaagt aaaccccgcg gcgtatagca gaggtggcgc accggtttat
     3961 gtggtggctg aatcagcttc gacgactgag tgggctgctc aatttggcct accgatgata
     4021 ttaagttgga ttataaatac taacgaaaag aaagcacaac ttgagcttta taatgaagtg
     4081 gctcaagaat atgggcacga tattcataat atcgaccatt gcttatcata tataacatct
     4141 gtagatcatg actcaattaa agcgaaagag atttgccgga aatttctggg gcattggtat
     4201 gattcttatg tgaatgctac gactattttt gatgattcag accaaacaag aggttatgat
     4261 ttcaataaag ggcagtggcg tgactttgta ttaaaaggac ataaagatac taatcgccgt
     4321 attgattaca gttacgaaat caatcccgtg ggaacgccgc aggaatgtat tgacataatt
     4381 caaaaagaca ttgatgctac aggaatatca aatatttgtt gtggatttga agctaatgga
     4441 acagtagacg aaattattgc ttccatgaag ctcttccagt ctgatgtcat gccatttctt
     4501 aaagaaaaac aacgttcgct attatattag ctaaggagaa agaaatgaaa tttggattgt
     4561 tcttccttaa cttcatcaat tcaacaactg ttcaagaaca aagtatagtt cgcatgcagg
     4621 aaataacgga gtatgttgat aagttgaatt ttgaacagat tttagtgtat gaaaatcatt
     4681 tttcagataa tggtgttgtc ggcgctcctc tgactgtttc tggttttctg ctcggtttaa
     4741 cagagaaaat taaaattggt tcattaaatc acatcattac aactcatcat cctgtccgca
     4801 tagcggagga agcttgctta ttggatcagt taagtgaagg gagatttatt ttagggttta
     4861 gtgattgcga aaaaaaagat gaaatgcatt tttttaatcg cccggttgaa tatcaacagc
     4921 aactatttga agagtgttat gaaatcatta acgatgcttt aacaacaggc tattgtaatc
     4981 cagataacga tttttatagc ttccctaaaa tatctgtaaa tccccatgct tatacgccag
     5041 gcggacctcg gaaatatgta acagcaacca gtcatcatat tgttgagtgg gcggccaaaa
     5101 aaggtattcc tctcatcttt aagtgggatg attctaatga tgttagatat gaatatgctg
     5161 aaagatataa agccgttgcg gataaatatg acgttgacct atcagagata gaccatcagt
     5221 taatgatatt agttaactat aacgaagata gtaataaagc taaacaagag acgcgtgcat
     5281 ttattagtga ttatgttctt gaaatgcacc ctaatgaaaa tttcgaaaat aaacttgaag
     5341 aaataattgc agaaaacgct gtcggaaatt atacggagtg tataactgcg gctaagttgg
     5401 caattgaaaa gtgtggtgcg aaaagtgtat tgctgtcctt tgaaccaatg aatgatttga
     5461 tgagccaaaa aaatgtaatc aatattgttg atgataatat taagaagtac cacatggaat
     5521 atacctaata gatttcgagt tgcagcgagg cggcaagtga acgaatcccc aggagcatag
     5581 ataactatgt gactggggtg agtgaaagca gccaacaaag cagcagcttg aaagatgaag
     5641 ggtataaaag agtatgacag cagtgctgcc atactttcta atattatctt gaggagtaaa
     5701 acaggtatga cttcatatgt tgataaacaa gaaattacag caagctcaga aattgatgat
     5761 ttgatttttt cgagcgatcc attagtgtgg tcttacgacg agcaggaaaa aatcagaaag
     5821 aaacttgtgc ttgatgcatt tcgtaatcat tataaacatt gtcgagaata tcgtcactac
     5881 tgtcaggcac acaaagtaga tgacaatatt acggaaattg atgacatacc tgtattccca
     5941 acatcggttt ttaagtttac tcgcttatta acttctcagg aaaacgagat tgaaagttgg
     6001 tttaccagta gcggcacgaa tggtttaaaa agtcaggtgg cgcgtgacag attaagtatt
     6061 gagagactct taggctctgt gagttatggc atgaaatatg ttggtagttg gtttgatcat
     6121 caaatagaat tagtcaattt gggaccagat agatttaatg ctcataatat ttggtttaaa
     6181 tatgttatga gtttggtgga attgttatat cctacgacat ttaccgtaac agaagaacga
     6241 atagattttg ttaaaacatt gaatagtctt gaacgaataa aaaatcaagg gaaagatctt
     6301 tgtcttattg gttcgccata ctttatttat ttactctgcc attatatgaa agataaaaaa
     6361 atctcatttt ctggagataa aagcctttat atcataaccg gaggcggctg gaaaagttac
     6421 gaaaaagaat ctctgaaacg tgatgatttc aatcatcttt tatttgatac tttcaatctc
     6481 agtgatatta gtcagatccg agatatattt aatcaagttg aactcaacac ttgtttcttt
     6541 gaggatgaaa tgcagcgtaa acatgttccg ccgtgggtat atgcgcgagc gcttgatcct
     6601 gaaacgttga aacctgtacc tgatggaacg ccggggttga tgagttatat ggatgcgtca
     6661 gcaaccagtt atccagcatt tattgttacc gatgatgtcg ggataattag cagagaatat
     6721 ggtaagtatc ccggcgtgct cgttgaaatt ttacgtcgcg tcaatacgag gacgcagaaa
     6781 gggtgtgctt taagcttaac cgaagcgttt gatagttgat gacctgcagg catgcaagct
     6841 tgcggccgcg ggccccatgg atcgatagct ggtcgacaca atctgccctt tcgaaagatc
     6901 ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac
     6961 atggcatgga tgagctctac aaataatgag ctagcccgcc taatgagcgg gctttttttt
     7021 ctcggcctag gagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat
     7081 aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac
     7141 ccgacaggac tataaagata ccaggcgttt ccccctggcg gctcccccgt gcgctctcct
     7201 gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc
     7261 acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc
     7321 ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga
     7381 aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct
     7441 tgaagccatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc
     7501 caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct
     7561 gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag
     7621 aagatcatct tattaatcag ataaaatatt tctaggatct aaaacactag gccccgtcag
     7681 gatggccttc tgcttaattt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     7741 tccgggccgt tgcttcgcaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     7801 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
     7861 ttttatttga tgcctggcag ttccctactc tcgcatgggg agaccccaca ctaccatcgg
     7921 cgctacggcg tttcacttct gagttcggca tggggtcagg tgggaccacc gcgctactgc
     7981 cgccaggcaa attctgtttt atcagaccgc ttctgcgttc tgatttaatc tgtatcaggc
     8041 tgaaaatctt ctctcatccg ccaaaacagc caagctggat ctggcaaatc gctgaatatt
     8101 ccttttgtct ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc
     8161 tgcgctcacg gctctggcag tgaatggggg taaatggcac tacaggcgcc ttttatggat
     8221 tcatgcaagg aaactaccca taatacaaga aaagcccgtc acgggcttct cagggcgttt
     8281 tatggcgggt ctgctatgtg gtgctatctg actttttgct gttcagcagt tcctgccctc
     8341 tgattttcca gtctgaccac ttcggattat cccgtgacag gtcattcaga ctggctaatg
     8401 cacccagtaa ggcagcggta tcatcaacag gcttacccgt cttactgtca accggatcta
     8461 aaacactagt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8521 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8581 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8641 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8701 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8761 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8821 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8881 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8941 attcagctcc ggttcccaac gatcaaggcg agttacatgg tcccccatgt tgtgcaaaaa
     9001 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9061 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9121 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9181 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9241 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9301 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9361 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9421 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9481 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9541 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     9601 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttctag acaacatcag
     9661 caaggagaaa ggggctaccg gcgaaccagc agccccttta taaaggcgct tcagtagtca
     9721 gaccagcatc agtcctgaaa aggcgggcct gcgcccgcct ccaggttgct acttaccgga
     9781 ttcgtaagcc atgaaagccg ccacctccct gtgtccgtct ctgtaacgaa tctcgcacag
     9841 cgattttcgt gtcagataag tgaatatcaa cagtgtgaga cacacgatca acacacacca
     9901 gacaagggaa cttcgtggta gtttcatggc cttcttctcc ttgcgcaaag cgcggtaaga
     9961 ggctatcctg atgtggacta gacataggga tgcctcgtgg tggttaatga aaattaactt
    10021 actacggggc tatcttcttt ctgccacaca acacggcaac aaaccacctt cacgtcatga
    10081 ggcagaaagc ctcaagcgcc gggcacatca tagcccatat acctgcacgc tgaccacact
    10141 cactttccct gaaaataatc cgctcattca gaccgttcac gggaaatccg tgtgattgtt
    10201 gccgcatcac gctgcctccc ggagtttgtc tcgagcactt ttgttacccg ccaaacaaaa
    10261 cccaaaaaca actcataccc aacccaataa aacaccaaaa caagacaaat aatcattgat
    10321 tgatggttga aatggggtaa acttgacaaa caaacccact taaaacccaa aacataccca
    10381 aacacacacc aaaaaaacac cataaggagt tttataaatg ttggtattca ttgatgacgg
    10441 ttcaacaaac atcaaactac agtagcagga aagcgacgga acaattaaac agcacattag
    10501 cccgaacagc ttcaaacgcg agtgggcagt ctcttttggt gataaaaagg tctttaacta
    10561 cacactgaac ggcgaacagt attcatttga tccaatcagc ccggatgctg tagtcacaac
    10621 caatatcgca tggcaataca gcgacgttaa tgtcgttgca gtgcatcacg ccttactgac
    10681 cagtggtctg ccggtaagcg aagtggatat tgtttgcaca cttcctctga cagagtatta
    10741 cgacagaaat aaccaaccca atacggaaaa tattgagcgt aagaaagcaa acttccggaa
    10801 aaaaattaca ttaaatggcg gggatacatt cacaataaaa gatgtaaaag tcatgcctga
    10861 atctataccg gcaggttatg aagttctaca agaactggat gagttagatt ctttattaat
    10921 tatagatctc gggggcacca cattagatat ttctcaggta atggggaaat tatcggggat
    10981 cagtaaaata tacggagact catctcttgg tgtctctctg gttacatctg cagtaaaaga
    11041 tgccctttct cttgcgagaa caaaaggaag tagctatctt gctgacgata taatcattca
    11101 cagaaaaga
//
