LOCUS       sequence-240669-        2709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  P-factor GPCR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS626
FEATURES             Location/Qualifiers
     source          1..2709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1076..1110)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1181..1840)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1755..1774
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2022..2610
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2511..2530
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc atatgaggca accttggtgg aaagacttta ctattcccga tgcaagcgca
       61 attattcacc aaaatattac cattgtgtct attgtcggag agattgaagt gcctgtttca
      121 acaattgatg catatgaaag ggataggtta ttaactggaa tgactttatc tgcccaatta
      181 gctttaggag tgttaaccat tttaatggtt tgtctgttat catcaagcga aaaacgtaaa
      241 caccctgttt ttgtttttaa ttcggcaagt attgttgcaa tgtgtttacg cgccatttta
      301 aatatcgtga ccatctgctc gaattcgtac agtatcctcg ttaattacgg gtttatctta
      361 aacatggttc atatgtatgt gcatgtgttt aatattttaa ttttattatt agcaccggtg
      421 atcattttta ctgctgagat gtcgatgatg attcaagtta ggatcatttg tgcacatgat
      481 aggaaaacac aacgtatcat gactgttatt agtgcctgct taactgtttt agttctcgca
      541 ttttggatta ctaacatgtg tcaacagatt cagtatctct tatggttaac tcctttatcg
      601 tcgaaaacca ttgttggata ctcttggccc tactttattg ctaaaatctt atttgctttt
      661 tcgattattt ttcacagtgg tgttttttca tacaaactct ttagggccat cttaatccgc
      721 aaaaaaattg ggcaatttcc ttttggtccg atgcagtgta ttttagttat ttcgtgccaa
      781 tgtttaattg ttcctgctac ctttactatc atcgatagtt ttatccatac gtatgatggc
      841 ttttcgtcta tgactcaatg tctcctgatc atttctttac cattatcgag tttatgggcg
      901 tctagtacag ctctcaaatt acaatcgatg aaaacttcat ctgcgcaagg agaaaccacc
      961 gaggtttcga ttagggttga taggacgttt gatatcaaac atactcccag tgacgattat
     1021 tcgatttctg atgaatctga aactaaaaag tggacgggat cctgagacca gaccaataaa
     1081 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     1141 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     1201 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1261 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     1321 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     1381 tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga
     1441 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     1501 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     1561 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     1621 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     1681 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     1741 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     1801 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     1861 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     1921 tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga gttttcgttc
     1981 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2041 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2101 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2161 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2221 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2281 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2341 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2401 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2461 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2521 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2581 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2641 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2701 gataaccgt
//
