LOCUS       sequence-240672-        2715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Melatonin GPCR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS1806
FEATURES             Location/Qualifiers
     source          1..2715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1082..1116)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1187..1846)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1761..1780
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2028..2616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2517..2536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc atatgcaagg taatggttct gctttgccaa atgcttctca accagttttg
       61 agaggtgatg gtgctagacc ttcttggttg gcttctgctt tagcttgtgt tttgattttc
      121 accatcgttg tcgatatctt gggtaacttg ttggttatct tgtccgtcta ccgtaacaag
      181 aaattgagaa acgctggtaa catcttcgtt gtttctttgg ctgttgctga tttggttgtt
      241 gctatctatc catatccact ggtcttgatg tccattttta acaacggttg gaacttgggt
      301 tacttgcatt gtcaagtttc tggtttcttg atgggtttgt ccgttattgg ttccattttc
      361 aacattaccg gtatcgccat taacaggtac tgttacattt gccactcact gaagtacgac
      421 aagttgtact cttctaagaa ctccttgtgc tacgttttgt tgatctggtt gttaactttg
      481 gctgctgttt tgcctaattt gagagctggt acattgcaat acgatccaag aatctactct
      541 tgtaccttcg ctcaatctgt ttcttctgct tacactattg ccgttgtcgt tttccatttt
      601 ttggtcccaa tgattatcgt catcttctgc tacttgagaa tctggatttt ggtcttgcaa
      661 gtcagacaaa gagttaagcc agatagaaag ccaaaattga agccacaaga cttcagaaac
      721 ttcgttacca tgtttgtggt tttcgttttg ttcgctattt gttgggctcc attgaacttt
      781 attggtttgg cagttgcttc tgatccagct tctatggttc caagaattcc agaatggttg
      841 ttcgttgctt cttactacat ggcttacttc aactcttgtt tgaacgccat tatctacggc
      901 ttgttgaatc agaactttag gaaagagtac aggcgtatca tcgtttcttt gtgtactgct
      961 agagttttct tcgtcgattc ctctaatgat gttgccgata gagttaagtg gaaaccatct
     1021 ccattgatga ccaacaacaa tgttgtcaag gttgactccg ttggatcctg agaccagacc
     1081 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     1141 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     1201 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     1261 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     1321 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     1381 aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt
     1441 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     1501 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     1561 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     1621 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     1681 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     1741 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     1801 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     1861 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     1921 gaaagttgga acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt
     1981 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2041 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2101 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2161 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2221 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2281 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2341 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2401 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2461 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2521 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2581 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2641 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2701 tctgtggata accgt
//
