LOCUS       sequence-240676-        2733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PbPeptide GPCR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS2119
FEATURES             Location/Qualifiers
     source          1..2733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1100..1134)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1205..1864)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1779..1798
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2046..2634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2535..2554
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc atatggcacc ctcattcgac cccttcaacc aaagcgtggt gttccacaag
       61 gccgacggaa ctccattcaa cgtgtcaatc catgaactag acgacttcgt gcagtacaac
      121 accaaagtct gcatcaacta ctcttcccag ctcggagcat ctgtcattgc aggactcatg
      181 cttgccatgc tgacacactc agaaaagcgt cgtctgccag ttttcttcct aaacacattc
      241 gcactggcca tgaactttgc ccgcctgctc tgcatgacca tctacttcac cacgggcttc
      301 aacaagtcct atgcctactt tggtcaggat tactcccagg tgcctgggag cgcctacgca
      361 gcctctgtct tgggcgttgt gttcaccact ctcctggtaa tcagcatgga aatgtccctc
      421 ctgatccaaa caagggttgt ctgcacgacc cttccggata tccaacgtta tctactcatg
      481 gcagtttcct ccgcgatttc cctgatggcc atcgggttcc gccttggctt aatggttgag
      541 aactgcattg ccattgtgca ggcgtcgaat ttcgcccctt ttatctggct tcaaagcgcc
      601 tcgaacatca ccattacgat cagcacatgt ttcttcagtg ccgtctttgt tacgaaattg
      661 gcatatgcac tcgtcactcg tatacgacta ggcttgacga ggtttggtgc tatgcaggtt
      721 atgttcatca tgtcctgcca gactatggtg attccagcca tcttctcaat tctccaatac
      781 ccactcccca agtacgaaat gaactccaac ctctttacgc tggtggccat tttcctccct
      841 ctttcctcgc tatgggcttc agttgctacg agatccagtt tcgagacatc ttcttccggc
      901 cgccatcagt atctttggcc aagcgaacag agcaataacg tcaccaattc ggaaattaag
      961 tatcaggtca gcttctctca gaaccacact acgttgcggt ctggagggtc tgtggccacg
     1021 acactctccc cggaccggct cgacccggtt tattgtgaag ttgaagctgg cacaaaggcc
     1081 ggatcctgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     1141 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     1201 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     1261 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     1321 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     1381 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca
     1441 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     1501 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     1561 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     1621 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     1681 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     1741 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     1801 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     1861 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     1921 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa
     1981 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2041 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2101 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2161 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2221 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2281 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2341 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2401 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2461 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2521 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2581 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2641 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2701 cctgcgttat cccctgattc tgtggataac cgt
//
