LOCUS       sequence-240679-        4080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. elegans tagged ribosome expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Rhoades JL, Nelson JC, Nwabudike I, Yu SK, McLachlan IG, Madan GK, 
            Abebe E, Powers JR, Colon-Ramos DA, Flavell SW
  TITLE     ASICs Mediate Food Responses in an Enteric Serotonergic Neuron that 
            Controls Foraging Behaviors.
  JOURNAL   Cell. 2019 Jan 10;176(1-2):85-97.e14. doi: 
            10.1016/j.cell.2018.11.023. Epub 2018 Dec 20.
  PUBMED    30580965
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2019-01-10"; volume: "176(1-2):85-97.e14. doi"; pages: " 
            10.1016/j.cell.2018.11.023. Epub 2018 Dec 20"
COMMENT     Sequence Label: tph-1(short)::rpl-22-HA
FEATURES             Location/Qualifiers
     source          1..4080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             712..801
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(1650..1672)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1772..1791
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1873..1891)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1959..2063
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2064..2132,2133..2924)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2282..2301)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3095..3683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3584..3603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3837..3854
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3971..3992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4007..4012,4013..4030,4031..4037)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4045..4061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4050..4072
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(4069..4080,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(4069..4080,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gcccgtgccg
       61 aattccagaa gcaccacgcc atcggatatc taaaagagga ggtgtctttg tttgcgcata
      121 ataaaacaat caatcaacac agcaaagacc cctctcaacc tcatttcatg attttctttg
      181 gtttttaggt agcattgctc tcttcaatca tggcgcgcct ctagaggatc cccgggattg
      241 gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga
      301 ggacccttgg ctagcatggt cccaaagcca cacgccaagt cggccaagaa ggctcttcgc
      361 aagaagaagg tgcacctcaa gttcaacgtt gagtgcaaga acccagttga ggatggaatt
      421 ctcagaatcg aagatcttga agccttcttg aacgagaaga tcaaggtcaa tggaaagacc
      481 ggacatttgg ccgctaacaa cgtcaaggtc gaggtcgcca agtccaaggt ctcagtcgtc
      541 tccgaggtcc cattctcgaa gagatacctc aagtacctta ccaagaagta cctcaagaga
      601 aactccctcc gtgattggct ccgtgtggtc gctgtcaaca agaacaccta cgaggtcaga
      661 tacttccaca tcaacgacgg agaggacgca ggaagcgacc acgaggaatt ctacccatac
      721 gatgttcctg actatgcggg ctatccctat gacgtcccgg actatgcagg atcctatcca
      781 tatgacgttc cagattacgc tgctcagtag ggtaccatgg tattgatatc tgagctccgc
      841 atcggccgct gtcatcagat cgccatctcg cgcccgtgcc tctgacttct aagtccaatt
      901 actcttcaac atccctacat gctctttctc cctgtgctcc caccccctat ttttgttatt
      961 atcaaaaaac ttctcttaat ttctttgttt tttagcttct tttaagtcac ctctaacaat
     1021 gaaattgtgt agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc
     1081 tccctccccc cattaataat aattctatcc caaaatctac acaatgttct gtgtacactt
     1141 cttatgtttt ttacttctga taaatttttt tgaaacatca tagaaaaaac cgcacacaaa
     1201 ataccttatc atatgttacg tttcagttta tgaccgcaat ttttatttct tcgcacgtct
     1261 gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta tttttggaat
     1321 ttttcaatca agtgaaagtt tatgaaatta attttcctgc ttttgctttt tggggtttcc
     1381 cctattgttt gtcaagattt cgaggacggc gtttttcttg ctaaaatcac aagtattgat
     1441 gagcacgatg caagaaagat cggaagaagg tttgggtttg aggctcagtg gaaggtgagt
     1501 agaagttgat aatttgaaag tggagtagtg tctatggggt ttttgcctta aatgacagaa
     1561 tacattccca atataccaaa cataactgtt tctactagtc ggccgtacgg gccctttcgt
     1621 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     1681 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     1741 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     1801 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgg
     1861 ccttaattaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     1921 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1981 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2041 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     2101 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2161 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2221 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2281 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2341 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2401 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2461 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2521 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2581 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2641 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2701 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2761 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2821 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     2881 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     2941 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3001 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3061 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3121 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3181 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3241 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3301 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3361 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3421 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3481 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3541 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3601 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3661 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3721 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3781 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3841 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     3901 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     3961 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     4021 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
//
