LOCUS       sequence-240683-        7400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression for funtional studies of cell growth promotion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Gaffney CJ, Oka T, Mazack V, Hilman D, Gat U, Muramatsu T, Inazawa 
            J, Golden A, Carey DJ, Farooq A, Tromp G, Sudol M
  TITLE     Identification, basic characterization and evolutionary analysis of 
            differentially spliced mRNA isoforms of human YAP1 gene.
  JOURNAL   Gene. 2012 Nov 10;509(2):215-22. doi: 10.1016/j.gene.2012.08.025. 
            Epub 2012 Aug 24.
  PUBMED    22939869
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gene.2012.08.025"; journalName: "Gene"; date: 
            "2012-11-10- 10"; volume: "509"; issue: "2"; pages: "215-22"
COMMENT     Sequence Label: pBABE (hygro) mEOS2 hYAP 1-2delta
FEATURES             Location/Qualifiers
     source          1..7400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     347..369
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     385..401
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             434..1111
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     primer_bind     complement(2656..2676)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2661..2990
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2841..2976
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3064..4089
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3064..4089
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     4606..4625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4887..5356
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(5471..6262,6263..6331))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6094..6113
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6332..6436)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             6467..6936
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    7001..7200
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
       61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
      241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccggc
      421 cggatccgcc accatgagtg cgattaagcc agacatgaag atcaaactcc gtatggaagg
      481 caacgtaaac gggcaccact ttgtgatcga cggagatggt acaggcaagc cttttgaggg
      541 aaaacagagt atggatcttg aagtcaaaga gggcggacct ctgccttttg cctttgatat
      601 cctgaccact gcattccatt acggcaacag ggtattcgcc aaatatccag acaacataca
      661 agactatttt aagcagtcgt ttcctaaggg gtattcgtgg gaacgaagct tgactttcga
      721 agacgggggc atttgcattg ccagaaacga cataacaatg gaaggggaca ctttctataa
      781 taaagttcga ttttatggta ccaactttcc cgccaatggt ccagttatgc agaagaagac
      841 gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt gatggagtgc tgacgggtga
      901 tattcatatg gctttgttgc ttgaaggaaa tgcccattac cgatgtgact tcagaactac
      961 ttacaaagct aaggagaagg gtgtcaagtt accaggctac cactttgtgg accactgcat
     1021 tgagatttta agccatgaca aagattacaa caaggttaag ctgtatgagc atgctgttgc
     1081 tcattctgga ttgcctgaca atgccagacg agaattcatg gatcccgggc agcagccgcc
     1141 gcctcaaccg gccccccagg gccaagggca gccgccttcg cagcccccgc aggggcaggg
     1201 cccgccgtcc ggacccgggc aaccggcacc cgcggcgacc caggcggcgc cgcaggcacc
     1261 ccccgccggg catcagatcg tgcacgtccg cggggactcg gagaccgacc tggaggcgct
     1321 cttcaacgcc gtcatgaacc ccaagacggc caacgtgccc cagaccgtgc ccatgaggct
     1381 ccggaagctg cccgactcct tcttcaagcc gccggagccc aaatcccact cccgacaggc
     1441 cagtactgat gcaggcactg caggagccct gactccacag catgttcgag ctcattcctc
     1501 tccagcttct ctgcagttgg gagctgtttc tcctgggaca ctgaccccca ctggagtagt
     1561 ctctggccca gcagctacac ccacagctca gcatcttcga cagtcttctt ttgagatacc
     1621 tgatgatgta cctctgccag caggttggga gatggcaaag acatcttctg gtcagagata
     1681 cttcttaaat cacatcgatc agacaacaac atggcaggac cccaggaagg ccatgctgtc
     1741 ccagatgaac gtcacagccc ccaccagtcc accagtgcag cagaatatga tgaactcggc
     1801 ttcaggtcct cttcctgatg gatgggaaca agccatgact caggatggag aaatttacta
     1861 tataaaccat aagaacaaga ccacctcttg gctagaccca aggcttgacc ctcgttttgc
     1921 catgaaccag agaatcagtc agagtgctcc agtgaaacag ccaccacccc tggctcccca
     1981 gagcccacag ggaggcgtca tgggtggcag caactccaac cagcagcaac agatgcgact
     2041 gcagcaactg cagatggaga aggagaggct gcggctgaaa cagcaagaac tgcttcggca
     2101 ggtgaggcca caggcaatgc ggaatatcaa tcccagcaca gcaaattctc caaaatgtca
     2161 ggagttagcc ctgcgtagcc agttaccaac actggagcag gatggtggga ctcaaaatcc
     2221 agtgtcttct cccgggatgt ctcaggaatt gagaacaatg acgaccaata gctcagatcc
     2281 tttccttaac agtggcacct atcactctcg agatgagagt acagacagtg gactaagcat
     2341 gagcagctac agtgtccctc gaaccccaga tgacttcctg aacagtgtgg atgagatgga
     2401 tacaggtgat actatcaacc aaagcaccct gccctcacag cagaaccgtt tcccagacta
     2461 ccttgaagcc attcctggga caaatgtgga ccttggaaca ctggaaggag atggaatgaa
     2521 catagaagga gaggagctga tgccaagtct gcaggaagct ttgagttctg acatccttaa
     2581 tgacatggag tctgttttgg ctgccaccaa gctagataaa gaaagctttc ttacatggtt
     2641 ataggtcgac cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     2701 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     2761 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     2821 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2881 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     2941 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttgcca
     3001 gctggggcgc cctctggtaa ggttgggaag ccctgcagat ctgatcccgg ggggcaatga
     3061 gatatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     3121 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     3181 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     3241 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     3301 attggggaat ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     3361 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     3421 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     3481 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     3541 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     3601 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     3661 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     3721 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     3781 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     3841 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     3901 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     3961 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     4021 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     4081 aaggaataga gtagatgccg accgggatct atcgatgcgg cgggtgtggt ggttacgcgt
     4141 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4201 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4261 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4321 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4381 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4441 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4501 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4561 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4621 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4681 gtcagggggg cggagcctat ggaaaaacgc cagcaacgca tcgataaaat aaaagatttt
     4741 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
     4801 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag
     4861 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
     4921 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag
     4981 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
     5041 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
     5101 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     5161 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag
     5221 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     5281 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     5341 cagcgggggt ctttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta
     5401 tttacattaa atggccatag tgcggccgca aatcaatcta aagtatatat gagtaaactt
     5461 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5521 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5581 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5641 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5701 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5761 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5821 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5881 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5941 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6001 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6061 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6121 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6181 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6241 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6301 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6361 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6421 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgcagcctga atatgggcca
     6481 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca
     6541 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     6601 agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg
     6661 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     6721 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     6781 cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca
     6841 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
     6901 ctgagtgatt gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg
     6961 agacccctgc ccagggacca ccgacccacc accgggaggc aagctggcca gcaacttatc
     7021 tgtgtctgtc cgattgtcta gtgtctatga ctgattttat gcgcctgcgt cggtactagt
     7081 tagctaacta gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg
     7141 gccgcaaccc tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag
     7201 gaagggagtc gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga
     7261 acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg
     7321 cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc
     7381 tgtatttgtc tgaaaattag
//
