LOCUS       sequence-240685-        7286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression for funtional studies of cell growth promotion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Gaffney CJ, Oka T, Mazack V, Hilman D, Gat U, Muramatsu T, Inazawa 
            J, Golden A, Carey DJ, Farooq A, Tromp G, Sudol M
  TITLE     Identification, basic characterization and evolutionary analysis of 
            differentially spliced mRNA isoforms of human YAP1 gene.
  JOURNAL   Gene. 2012 Nov 10;509(2):215-22. doi: 10.1016/j.gene.2012.08.025. 
            Epub 2012 Aug 24.
  PUBMED    22939869
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gene.2012.08.025"; journalName: "Gene"; date: 
            "2012-11-10- 10"; volume: "509"; issue: "2"; pages: "215-22"
COMMENT     Sequence Label: pBABE (hygro) mEOS2 hYAP 1-1delta
FEATURES             Location/Qualifiers
     source          1..7286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             17..694
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     primer_bind     complement(2125..2145)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2130..2459
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2310..2445
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2533..3558
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2533..3558
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     4075..4094
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4356..4825
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(4940..5731,5732..5800))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5563..5582
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5801..5905)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5936..6405
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    6470..6669
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             6870..7286
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7216..7238
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7254..7270
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 ggccggatcc gccaccatga gtgcgattaa gccagacatg aagatcaaac tccgtatgga
       61 aggcaacgta aacgggcacc actttgtgat cgacggagat ggtacaggca agccttttga
      121 gggaaaacag agtatggatc ttgaagtcaa agagggcgga cctctgcctt ttgcctttga
      181 tatcctgacc actgcattcc attacggcaa cagggtattc gccaaatatc cagacaacat
      241 acaagactat tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa gcttgacttt
      301 cgaagacggg ggcatttgca ttgccagaaa cgacataaca atggaagggg acactttcta
      361 taataaagtt cgattttatg gtaccaactt tcccgccaat ggtccagtta tgcagaagaa
      421 gacgctgaaa tgggagccct ccactgagaa aatgtatgtg cgtgatggag tgctgacggg
      481 tgatattcat atggctttgt tgcttgaagg aaatgcccat taccgatgtg acttcagaac
      541 tacttacaaa gctaaggaga agggtgtcaa gttaccaggc taccactttg tggaccactg
      601 cattgagatt ttaagccatg acaaagatta caacaaggtt aagctgtatg agcatgctgt
      661 tgctcattct ggattgcctg acaatgccag acgagaattc atggatcccg ggcagcagcc
      721 gccgcctcaa ccggcccccc agggccaagg gcagccgcct tcgcagcccc cgcaggggca
      781 gggcccgccg tccggacccg ggcaaccggc acccgcggcg acccaggcgg cgccgcaggc
      841 accccccgcc gggcatcaga tcgtgcacgt ccgcggggac tcggagaccg acctggaggc
      901 gctcttcaac gccgtcatga accccaagac ggccaacgtg ccccagaccg tgcccatgag
      961 gctccggaag ctgcccgact ccttcttcaa gccgccggag cccaaatccc actcccgaca
     1021 ggccagtact gatgcaggca ctgcaggagc cctgactcca cagcatgttc gagctcattc
     1081 ctctccagct tctctgcagt tgggagctgt ttctcctggg acactgaccc ccactggagt
     1141 agtctctggc ccagcagcta cacccacagc tcagcatctt cgacagtctt cttttgagat
     1201 acctgatgat gtacctctgc cagcaggttg ggagatggca aagacatctt ctggtcagag
     1261 atacttctta aatcacatcg atcagacaac aacatggcag gaccccagga aggccatgct
     1321 gtcccagatg aacgtcacag cccccaccag tccaccagtg cagcagaata tgatgaactc
     1381 ggcttcagcc atgaaccaga gaatcagtca gagtgctcca gtgaaacagc caccacccct
     1441 ggctccccag agcccacagg gaggcgtcat gggtggcagc aactccaacc agcagcaaca
     1501 gatgcgactg cagcaactgc agatggagaa ggagaggctg cggctgaaac agcaagaact
     1561 gcttcggcag gtgaggccac aggcaatgcg gaatatcaat cccagcacag caaattctcc
     1621 aaaatgtcag gagttagccc tgcgtagcca gttaccaaca ctggagcagg atggtgggac
     1681 tcaaaatcca gtgtcttctc ccgggatgtc tcaggaattg agaacaatga cgaccaatag
     1741 ctcagatcct ttccttaaca gtggcaccta tcactctcga gatgagagta cagacagtgg
     1801 actaagcatg agcagctaca gtgtccctcg aaccccagat gacttcctga acagtgtgga
     1861 tgagatggat acaggtgata ctatcaacca aagcaccctg ccctcacagc agaaccgttt
     1921 cccagactac cttgaagcca ttcctgggac aaatgtggac cttggaacac tggaaggaga
     1981 tggaatgaac atagaaggag aggagctgat gccaagtctg caggaagctt tgagttctga
     2041 catccttaat gacatggagt ctgttttggc tgccaccaag ctagataaag aaagctttct
     2101 tacatggtta taggtcgacc ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     2161 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     2221 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2281 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     2341 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     2401 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     2461 agcttgccag ctggggcgcc ctctggtaag gttgggaagc cctgcagatc tgatcccggg
     2521 gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     2581 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     2641 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     2701 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     2761 gtgcttgaca ttggggaatt tagcgagagc ctgacctatt gcatctcccg ccgtgcacag
     2821 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
     2881 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     2941 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     3001 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     3061 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     3121 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     3181 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     3241 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     3301 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     3361 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     3421 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     3481 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     3541 ccgagggcaa aggaatagag tagatgccga ccgggatcta tcgatgcggc gggtgtggtg
     3601 gttacgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3661 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3721 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3781 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3841 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3901 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3961 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4021 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4081 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4141 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcat cgataaaata
     4201 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     4261 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga
     4321 gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc
     4381 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg
     4441 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     4501 tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
     4561 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
     4621 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
     4681 gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct
     4741 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
     4801 actacccgtc agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt
     4861 tcttaagtat ttacattaaa tggccatagt gcggccgcaa atcaatctaa agtatatatg
     4921 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     4981 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5041 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5101 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5161 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5221 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     5281 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     5341 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     5401 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     5461 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     5521 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     5581 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     5641 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     5701 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     5761 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     5821 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     5881 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gcagcctgaa
     5941 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     6001 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     6061 tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac
     6121 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     6181 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     6241 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
     6301 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     6361 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc
     6421 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca agctggccag
     6481 caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg cgcctgcgtc
     6541 ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttct
     6601 gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt ttttgtggcc
     6661 cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg gttctggtag
     6721 gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc
     6781 gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga
     6841 ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga
     6901 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
     6961 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
     7021 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
     7081 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
     7141 acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat
     7201 ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc
     7261 cagccctcac tccttctcta ggcgcc
//
