LOCUS       sequence-240696-        7226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression for funtional studies of cell growth promotion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Gaffney CJ, Oka T, Mazack V, Hilman D, Gat U, Muramatsu T, Inazawa 
            J, Golden A, Carey DJ, Farooq A, Tromp G, Sudol M
  TITLE     Identification, basic characterization and evolutionary analysis of 
            differentially spliced mRNA isoforms of human YAP1 gene.
  JOURNAL   Gene. 2012 Nov 10;509(2):215-22. doi: 10.1016/j.gene.2012.08.025. 
            Epub 2012 Aug 24.
  PUBMED    22939869
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gene.2012.08.025"; journalName: "Gene"; date: 
            "2012-11-10- 10"; volume: "509"; issue: "2"; pages: "215-22"
COMMENT     Sequence Label: pBABE (hygro) mEOS2 hYAP 1-1alpha
FEATURES             Location/Qualifiers
     source          1..7226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     347..369
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     385..401
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             434..1111
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     primer_bind     complement(2482..2502)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2487..2816
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2667..2802
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2890..3915
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2890..3915
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     4432..4451
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4713..5182
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(5297..6088,6089..6157))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5920..5939
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6158..6262)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             6293..6762
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    6827..7026
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
       61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
      241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccggc
      421 cggatccgcc accatgagtg cgattaagcc agacatgaag atcaaactcc gtatggaagg
      481 caacgtaaac gggcaccact ttgtgatcga cggagatggt acaggcaagc cttttgaggg
      541 aaaacagagt atggatcttg aagtcaaaga gggcggacct ctgccttttg cctttgatat
      601 cctgaccact gcattccatt acggcaacag ggtattcgcc aaatatccag acaacataca
      661 agactatttt aagcagtcgt ttcctaaggg gtattcgtgg gaacgaagct tgactttcga
      721 agacgggggc atttgcattg ccagaaacga cataacaatg gaaggggaca ctttctataa
      781 taaagttcga ttttatggta ccaactttcc cgccaatggt ccagttatgc agaagaagac
      841 gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt gatggagtgc tgacgggtga
      901 tattcatatg gctttgttgc ttgaaggaaa tgcccattac cgatgtgact tcagaactac
      961 ttacaaagct aaggagaagg gtgtcaagtt accaggctac cactttgtgg accactgcat
     1021 tgagatttta agccatgaca aagattacaa caaggttaag ctgtatgagc atgctgttgc
     1081 tcattctgga ttgcctgaca atgccagacg agaattcatg gatcccgggc agcagccgcc
     1141 gcctcaaccg gccccccagg gccaagggca gccgccttcg cagcccccgc aggggcaggg
     1201 cccgccgtcc ggacccgggc aaccggcacc cgcggcgacc caggcggcgc cgcaggcacc
     1261 ccccgccggg catcagatcg tgcacgtccg cggggactcg gagaccgacc tggaggcgct
     1321 cttcaacgcc gtcatgaacc ccaagacggc caacgtgccc cagaccgtgc ccatgaggct
     1381 ccggaagctg cccgactcct tcttcaagcc gccggagccc aaatcccact cccgacaggc
     1441 cagtactgat gcaggcactg caggagccct gactccacag catgttcgag ctcattcctc
     1501 tccagcttct ctgcagttgg gagctgtttc tcctgggaca ctgaccccca ctggagtagt
     1561 ctctggccca gcagctacac ccacagctca gcatcttcga cagtcttctt ttgagatacc
     1621 tgatgatgta cctctgccag caggttggga gatggcaaag acatcttctg gtcagagata
     1681 cttcttaaat cacatcgatc agacaacaac atggcaggac cccaggaagg ccatgctgtc
     1741 ccagatgaac gtcacagccc ccaccagtcc accagtgcag cagaatatga tgaactcggc
     1801 ttcagccatg aaccagagaa tcagtcagag tgctccagtg aaacagccac cacccctggc
     1861 tccccagagc ccacagggag gcgtcatggg tggcagcaac tccaaccagc agcaacagat
     1921 gcgactgcag caactgcaga tggagaagga gaggctgcgg ctgaaacagc aagaactgct
     1981 tcggcaggag ttagccctgc gtagccagtt accaacactg gagcaggatg gtgggactca
     2041 aaatccagtg tcttctcccg ggatgtctca ggaattgaga acaatgacga ccaatagctc
     2101 agatcctttc cttaacagtg gcacctatca ctctcgagat gagagtacag acagtggact
     2161 aagcatgagc agctacagtg tccctcgaac cccagatgac ttcctgaaca gtgtggatga
     2221 gatggataca ggtgatacta tcaaccaaag caccctgccc tcacagcaga accgtttccc
     2281 agactacctt gaagccattc ctgggacaaa tgtggacctt ggaacactgg aaggagatgg
     2341 aatgaacata gaaggagagg agctgatgcc aagtctgcag gaagctttga gttctgacat
     2401 ccttaatgac atggagtctg ttttggctgc caccaagcta gataaagaaa gctttcttac
     2461 atggttatag gtcgaccctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     2521 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     2581 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     2641 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     2701 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     2761 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     2821 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcagatctga tcccgggggg
     2881 caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     2941 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     3001 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     3061 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     3121 cttgacattg gggaatttag cgagagcctg acctattgca tctcccgccg tgcacagggt
     3181 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     3241 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     3301 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     3361 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     3421 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     3481 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     3541 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     3601 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     3661 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     3721 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     3781 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     3841 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     3901 agggcaaagg aatagagtag atgccgaccg ggatctatcg atgcggcggg tgtggtggtt
     3961 acgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4021 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4081 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4141 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4201 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4261 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4321 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4381 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4441 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4501 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcatcga taaaataaaa
     4561 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     4621 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa
     4681 gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     4741 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc
     4801 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
     4861 ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
     4921 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
     4981 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg
     5041 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc
     5101 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
     5161 acccgtcagc gggggtcttt cacatgcagc atgtatcaaa attaatttgg ttttttttct
     5221 taagtattta cattaaatgg ccatagtgcg gccgcaaatc aatctaaagt atatatgagt
     5281 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5341 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5401 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5461 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5521 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5581 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5641 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5701 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5761 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5821 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5881 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5941 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6001 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6061 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6121 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6181 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6241 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgca gcctgaatat
     6301 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     6361 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     6421 gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat
     6481 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
     6541 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
     6601 ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg
     6661 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
     6721 tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg
     6781 atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggcaagc tggccagcaa
     6841 cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt
     6901 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
     6961 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
     7021 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
     7081 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
     7141 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
     7201 tgtttctgta tttgtctgaa aattag
//
