LOCUS       sequence-240708-        7274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression for funtional studies of cell growth promotion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Gaffney CJ, Oka T, Mazack V, Hilman D, Gat U, Muramatsu T, Inazawa 
            J, Golden A, Carey DJ, Farooq A, Tromp G, Sudol M
  TITLE     Identification, basic characterization and evolutionary analysis of 
            differentially spliced mRNA isoforms of human YAP1 gene.
  JOURNAL   Gene. 2012 Nov 10;509(2):215-22. doi: 10.1016/j.gene.2012.08.025. 
            Epub 2012 Aug 24.
  PUBMED    22939869
REFERENCE   2  (bases 1 to 7274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gene.2012.08.025"; journalName: "Gene"; date: 
            "2012-11-10- 10"; volume: "509"; issue: "2"; pages: "215-22"
COMMENT     Sequence Label: pBABE (hygro)mEOS2 hYAP 1-1gamma
FEATURES             Location/Qualifiers
     source          1..7274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     347..369
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     385..401
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             434..1111
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     primer_bind     complement(2530..2550)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2535..2864
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2715..2850
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2938..3963
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2938..3963
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     4480..4499
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4761..5230
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(5345..6136,6137..6205))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5968..5987
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6206..6310)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             6341..6810
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    6875..7074
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
       61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
      241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccggc
      421 cggatccgcc accatgagtg cgattaagcc agacatgaag atcaaactcc gtatggaagg
      481 caacgtaaac gggcaccact ttgtgatcga cggagatggt acaggcaagc cttttgaggg
      541 aaaacagagt atggatcttg aagtcaaaga gggcggacct ctgccttttg cctttgatat
      601 cctgaccact gcattccatt acggcaacag ggtattcgcc aaatatccag acaacataca
      661 agactatttt aagcagtcgt ttcctaaggg gtattcgtgg gaacgaagct tgactttcga
      721 agacgggggc atttgcattg ccagaaacga cataacaatg gaaggggaca ctttctataa
      781 taaagttcga ttttatggta ccaactttcc cgccaatggt ccagttatgc agaagaagac
      841 gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt gatggagtgc tgacgggtga
      901 tattcatatg gctttgttgc ttgaaggaaa tgcccattac cgatgtgact tcagaactac
      961 ttacaaagct aaggagaagg gtgtcaagtt accaggctac cactttgtgg accactgcat
     1021 tgagatttta agccatgaca aagattacaa caaggttaag ctgtatgagc atgctgttgc
     1081 tcattctgga ttgcctgaca atgccagacg agaattcatg gatcccgggc agcagccgcc
     1141 gcctcaaccg gccccccagg gccaagggca gccgccttcg cagcccccgc aggggcaggg
     1201 cccgccgtcc ggacccgggc aaccggcacc cgcggcgacc caggcggcgc cgcaggcacc
     1261 ccccgccggg catcagatcg tgcacgtccg cggggactcg gagaccgacc tggaggcgct
     1321 cttcaacgcc gtcatgaacc ccaagacggc caacgtgccc cagaccgtgc ccatgaggct
     1381 ccggaagctg cccgactcct tcttcaagcc gccggagccc aaatcccact cccgacaggc
     1441 cagtactgat gcaggcactg caggagccct gactccacag catgttcgag ctcattcctc
     1501 tccagcttct ctgcagttgg gagctgtttc tcctgggaca ctgaccccca ctggagtagt
     1561 ctctggccca gcagctacac ccacagctca gcatcttcga cagtcttctt ttgagatacc
     1621 tgatgatgta cctctgccag caggttggga gatggcaaag acatcttctg gtcagagata
     1681 cttcttaaat cacatcgatc agacaacaac atggcaggac cccaggaagg ccatgctgtc
     1741 ccagatgaac gtcacagccc ccaccagtcc accagtgcag cagaatatga tgaactcggc
     1801 ttcagccatg aaccagagaa tcagtcagag tgctccagtg aaacagccac cacccctggc
     1861 tccccagagc ccacagggag gcgtcatggg tggcagcaac tccaaccagc agcaacagat
     1921 gcgactgcag caactgcaga tggagaagga gaggctgcgg ctgaaacagc aagaactgct
     1981 tcggcaggca atgcggaata tcaatcccag cacagcaaat tctccaaaat gtcaggagtt
     2041 agccctgcgt agccagttac caacactgga gcaggatggt gggactcaaa atccagtgtc
     2101 ttctcccggg atgtctcagg aattgagaac aatgacgacc aatagctcag atcctttcct
     2161 taacagtggc acctatcact ctcgagatga gagtacagac agtggactaa gcatgagcag
     2221 ctacagtgtc cctcgaaccc cagatgactt cctgaacagt gtggatgaga tggatacagg
     2281 tgatactatc aaccaaagca ccctgccctc acagcagaac cgtttcccag actaccttga
     2341 agccattcct gggacaaatg tggaccttgg aacactggaa ggagatggaa tgaacataga
     2401 aggagaggag ctgatgccaa gtctgcagga agctttgagt tctgacatcc ttaatgacat
     2461 ggagtctgtt ttggctgcca ccaagctaga taaagaaagc tttcttacat ggttataggt
     2521 cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     2581 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     2641 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     2701 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     2761 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     2821 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt gccagctggg
     2881 gcgccctctg gtaaggttgg gaagccctgc agatctgatc ccggggggca atgagatatg
     2941 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     3001 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     3061 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     3121 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     3181 gaatttagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     3241 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     3301 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     3361 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     3421 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     3481 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     3541 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     3601 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     3661 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     3721 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     3781 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     3841 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     3901 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     3961 tagagtagat gccgaccggg atctatcgat gcggcgggtg tggtggttac gcgtaatctg
     4021 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4081 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4141 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4201 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4261 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4321 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4381 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4441 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4501 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4561 ggggcggagc ctatggaaaa acgccagcaa cgcatcgata aaataaaaga ttttatttag
     4621 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     4681 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag
     4741 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     4801 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     4861 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     4921 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     4981 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     5041 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
     5101 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     5161 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     5221 gggtctttca catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca
     5281 ttaaatggcc atagtgcggc cgcaaatcaa tctaaagtat atatgagtaa acttggtctg
     5341 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5401 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5461 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5521 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5581 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5641 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5701 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5761 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5821 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5881 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5941 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6001 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6061 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6121 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6181 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6241 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6301 gggttccgcg cacatttccc cgaaaagtgc cacctgcagc ctgaatatgg gccaaacagg
     6361 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa
     6421 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     6481 gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca
     6541 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
     6601 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
     6661 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac
     6721 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
     6781 gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc
     6841 ctgcccaggg accaccgacc caccaccggg aggcaagctg gccagcaact tatctgtgtc
     6901 tgtccgattg tctagtgtct atgactgatt ttatgcgcct gcgtcggtac tagttagcta
     6961 actagctctg tatctggcgg acccgtggtg gaactgacga gttctgaaca cccggccgca
     7021 accctgggag acgtcccagg gactttgggg gccgtttttg tggcccgacc tgaggaaggg
     7081 agtcgatgtg gaatccgacc ccgtcaggat atgtggttct ggtaggagac gagaacctaa
     7141 aacagttccc gcctccgtct gaatttttgc tttcggtttg gaaccgaagc cgcgcgtctt
     7201 gtctgctgca gcgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt
     7261 tgtctgaaaa ttag
//
