LOCUS       sequence-240711-        6686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression for funtional studies of cell growth promotion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Gaffney CJ, Oka T, Mazack V, Hilman D, Gat U, Muramatsu T, Inazawa 
            J, Golden A, Carey DJ, Farooq A, Tromp G, Sudol M
  TITLE     Identification, basic characterization and evolutionary analysis of 
            differentially spliced mRNA isoforms of human YAP1 gene.
  JOURNAL   Gene. 2012 Nov 10;509(2):215-22. doi: 10.1016/j.gene.2012.08.025. 
            Epub 2012 Aug 24.
  PUBMED    22939869
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gene.2012.08.025"; journalName: "Gene"; date: 
            "2012-11-10- 10"; volume: "509"; issue: "2"; pages: "215-22"
COMMENT     Sequence Label: pBABE (hygro) hYAP 1-2beta
FEATURES             Location/Qualifiers
     source          1..6686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1525..1545)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1530..1859
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1710..1845
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1933..2958
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1933..2958
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     3475..3494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             3756..4225
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(4340..5131,5132..5200))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4963..4982
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5201..5305)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5336..5805
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5870..6069
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             6270..6686
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6616..6638
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6654..6670
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 ggccggatcc cagtgtggtg gtacgtagga attcatggat cccgggcagc agccgccgcc
       61 tcaaccggcc ccccagggcc aagggcagcc gccttcgcag cccccgcagg ggcagggccc
      121 gccgtccgga cccgggcaac cggcacccgc ggcgacccag gcggcgccgc aggcaccccc
      181 cgccgggcat cagatcgtgc acgtccgcgg ggactcggag accgacctgg aggcgctctt
      241 caacgccgtc atgaacccca agacggccaa cgtgccccag accgtgccca tgaggctccg
      301 gaagctgccc gactccttct tcaagccgcc ggagcccaaa tcccactccc gacaggccag
      361 tactgatgca ggcactgcag gagccctgac tccacagcat gttcgagctc attcctctcc
      421 agcttctctg cagttgggag ctgtttctcc tgggacactg acccccactg gagtagtctc
      481 tggcccagca gctacaccca cagctcagca tcttcgacag tcttcttttg agatacctga
      541 tgatgtacct ctgccagcag gttgggagat ggcaaagaca tcttctggtc agagatactt
      601 cttaaatcac atcgatcaga caacaacatg gcaggacccc aggaaggcca tgctgtccca
      661 gatgaacgtc acagccccca ccagtccacc agtgcagcag aatatgatga actcggcttc
      721 aggtcctctt cctgatggat gggaacaagc catgactcag gatggagaaa tttactatat
      781 aaaccataag aacaagacca cctcttggct agacccaagg cttgaccctc gttttgccat
      841 gaaccagaga atcagtcaga gtgctccagt gaaacagcca ccacccctgg ctccccagag
      901 cccacaggga ggcgtcatgg gtggcagcaa ctccaaccag cagcaacaga tgcgactgca
      961 gcaactgcag atggagaagg agaggctgcg gctgaaacag caagaactgc ttcggcaggt
     1021 gaggccacag gagttagccc tgcgtagcca gttaccaaca ctggagcagg atggtgggac
     1081 tcaaaatcca gtgtcttctc ccgggatgtc tcaggaattg agaacaatga cgaccaatag
     1141 ctcagatcct ttccttaaca gtggcaccta tcactctcga gatgagagta cagacagtgg
     1201 actaagcatg agcagctaca gtgtccctcg aaccccagat gacttcctga acagtgtgga
     1261 tgagatggat acaggtgata ctatcaacca aagcaccctg ccctcacagc agaaccgttt
     1321 cccagactac cttgaagcca ttcctgggac aaatgtggac cttggaacac tggaaggaga
     1381 tggaatgaac atagaaggag aggagctgat gccaagtctg caggaagctt tgagttctga
     1441 catccttaat gacatggagt ctgttttggc tgccaccaag ctagataaag aaagctttct
     1501 tacatggtta taggtcgacc ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     1561 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     1621 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     1681 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     1741 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     1801 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     1861 agcttgccag ctggggcgcc ctctggtaag gttgggaagc cctgcagatc tgatcccggg
     1921 gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     1981 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     2041 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     2101 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     2161 gtgcttgaca ttggggaatt tagcgagagc ctgacctatt gcatctcccg ccgtgcacag
     2221 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
     2281 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     2341 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     2401 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     2461 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     2521 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     2581 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     2641 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     2701 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     2761 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     2821 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     2881 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     2941 ccgagggcaa aggaatagag tagatgccga ccgggatcta tcgatgcggc gggtgtggtg
     3001 gttacgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3061 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3121 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3181 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3241 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3301 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3361 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3421 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3481 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3541 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcat cgataaaata
     3601 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     3661 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga
     3721 gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc
     3781 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg
     3841 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     3901 tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
     3961 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
     4021 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
     4081 gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct
     4141 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
     4201 actacccgtc agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt
     4261 tcttaagtat ttacattaaa tggccatagt gcggccgcaa atcaatctaa agtatatatg
     4321 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     4381 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     4441 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     4501 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     4561 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4621 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     4681 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     4741 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4801 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4861 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4921 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     4981 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     5041 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     5101 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     5161 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     5221 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     5281 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gcagcctgaa
     5341 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     5401 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     5461 tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac
     5521 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     5581 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     5641 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
     5701 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     5761 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc
     5821 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca agctggccag
     5881 caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg cgcctgcgtc
     5941 ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttct
     6001 gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt ttttgtggcc
     6061 cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg gttctggtag
     6121 gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc
     6181 gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga
     6241 ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga
     6301 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
     6361 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
     6421 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
     6481 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
     6541 acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat
     6601 ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc
     6661 cagccctcac tccttctcta ggcgcc
//
