LOCUS       sequence-240715-        6722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression for funtional studies of cell growth promotion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Gaffney CJ, Oka T, Mazack V, Hilman D, Gat U, Muramatsu T, Inazawa 
            J, Golden A, Carey DJ, Farooq A, Tromp G, Sudol M
  TITLE     Identification, basic characterization and evolutionary analysis of 
            differentially spliced mRNA isoforms of human YAP1 gene.
  JOURNAL   Gene. 2012 Nov 10;509(2):215-22. doi: 10.1016/j.gene.2012.08.025. 
            Epub 2012 Aug 24.
  PUBMED    22939869
REFERENCE   2  (bases 1 to 6722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gene.2012.08.025"; journalName: "Gene"; date: 
            "2012-11-10- 10"; volume: "509"; issue: "2"; pages: "215-22"
COMMENT     Sequence Label: pBABE (hygro) hYAP 1-2gamma
FEATURES             Location/Qualifiers
     source          1..6722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    209..408
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             609..1025
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     955..977
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     993..1009
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2586..2606)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2591..2920
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2771..2906
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2994..4019
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2994..4019
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     4536..4555
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4817..5286
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(5401..6192,6193..6261))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6024..6043
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6262..6366)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             join(6397..6722,1..144)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
       61 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
      121 ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc
      181 agggaccacc gacccaccac cgggaggcaa gctggccagc aacttatctg tgtctgtccg
      241 attgtctagt gtctatgact gattttatgc gcctgcgtcg gtactagtta gctaactagc
      301 tctgtatctg gcggacccgt ggtggaactg acgagttctg aacacccggc cgcaaccctg
      361 ggagacgtcc cagggacttt gggggccgtt tttgtggccc gacctgagga agggagtcga
      421 tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg agacgagaac ctaaaacagt
      481 tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc
      541 tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg
      601 aaaattaggg ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg
      661 tcgagcggat cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct
      721 gctctgcaga atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag
      781 acctcatcac ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc
      841 aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc
      901 cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg
      961 aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag
     1021 gcgccggccg gatcccagtg tggtggtacg taggaattca tggatcccgg gcagcagccg
     1081 ccgcctcaac cggcccccca gggccaaggg cagccgcctt cgcagccccc gcaggggcag
     1141 ggcccgccgt ccggacccgg gcaaccggca cccgcggcga cccaggcggc gccgcaggca
     1201 ccccccgccg ggcatcagat cgtgcacgtc cgcggggact cggagaccga cctggaggcg
     1261 ctcttcaacg ccgtcatgaa ccccaagacg gccaacgtgc cccagaccgt gcccatgagg
     1321 ctccggaagc tgcccgactc cttcttcaag ccgccggagc ccaaatccca ctcccgacag
     1381 gccagtactg atgcaggcac tgcaggagcc ctgactccac agcatgttcg agctcattcc
     1441 tctccagctt ctctgcagtt gggagctgtt tctcctggga cactgacccc cactggagta
     1501 gtctctggcc cagcagctac acccacagct cagcatcttc gacagtcttc ttttgagata
     1561 cctgatgatg tacctctgcc agcaggttgg gagatggcaa agacatcttc tggtcagaga
     1621 tacttcttaa atcacatcga tcagacaaca acatggcagg accccaggaa ggccatgctg
     1681 tcccagatga acgtcacagc ccccaccagt ccaccagtgc agcagaatat gatgaactcg
     1741 gcttcaggtc ctcttcctga tggatgggaa caagccatga ctcaggatgg agaaatttac
     1801 tatataaacc ataagaacaa gaccacctct tggctagacc caaggcttga ccctcgtttt
     1861 gccatgaacc agagaatcag tcagagtgct ccagtgaaac agccaccacc cctggctccc
     1921 cagagcccac agggaggcgt catgggtggc agcaactcca accagcagca acagatgcga
     1981 ctgcagcaac tgcagatgga gaaggagagg ctgcggctga aacagcaaga actgcttcgg
     2041 caggcaatgc ggaatatcaa tcccagcaca gcaaattctc caaaatgtca ggagttagcc
     2101 ctgcgtagcc agttaccaac actggagcag gatggtggga ctcaaaatcc agtgtcttct
     2161 cccgggatgt ctcaggaatt gagaacaatg acgaccaata gctcagatcc tttccttaac
     2221 agtggcacct atcactctcg agatgagagt acagacagtg gactaagcat gagcagctac
     2281 agtgtccctc gaaccccaga tgacttcctg aacagtgtgg atgagatgga tacaggtgat
     2341 actatcaacc aaagcaccct gccctcacag cagaaccgtt tcccagacta ccttgaagcc
     2401 attcctggga caaatgtgga ccttggaaca ctggaaggag atggaatgaa catagaagga
     2461 gaggagctga tgccaagtct gcaggaagct ttgagttctg acatccttaa tgacatggag
     2521 tctgttttgg ctgccaccaa gctagataaa gaaagctttc ttacatggtt ataggtcgac
     2581 cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     2641 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     2701 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     2761 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     2821 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     2881 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttgcca gctggggcgc
     2941 cctctggtaa ggttgggaag ccctgcagat ctgatcccgg ggggcaatga gatatgaaaa
     3001 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     3061 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     3121 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     3181 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     3241 ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     3301 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     3361 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     3421 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     3481 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     3541 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     3601 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     3661 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     3721 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     3781 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     3841 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     3901 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     3961 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga
     4021 gtagatgccg accgggatct atcgatgcgg cgggtgtggt ggttacgcgt aatctgctgc
     4081 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4141 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4201 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4261 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4321 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4381 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4441 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4501 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4561 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4621 cggagcctat ggaaaaacgc cagcaacgca tcgataaaat aaaagatttt atttagtctc
     4681 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg
     4741 ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca
     4801 ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     4861 cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     4921 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc
     4981 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
     5041 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
     5101 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt
     5161 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
     5221 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
     5281 ctttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta tttacattaa
     5341 atggccatag tgcggccgca aatcaatcta aagtatatat gagtaaactt ggtctgacag
     5401 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5461 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5521 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5581 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5641 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5701 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5761 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5821 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5881 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5941 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     6001 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     6061 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     6121 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     6181 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     6241 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     6301 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     6361 tccgcgcaca tttccccgaa aagtgccacc tgcagcctga atatgggcca aacaggatat
     6421 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg
     6481 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     6541 gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt
     6601 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
     6661 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
     6721 gg
//
