LOCUS       sequence-240716-        6674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression for funtional studies of cell growth promotion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Gaffney CJ, Oka T, Mazack V, Hilman D, Gat U, Muramatsu T, Inazawa 
            J, Golden A, Carey DJ, Farooq A, Tromp G, Sudol M
  TITLE     Identification, basic characterization and evolutionary analysis of 
            differentially spliced mRNA isoforms of human YAP1 gene.
  JOURNAL   Gene. 2012 Nov 10;509(2):215-22. doi: 10.1016/j.gene.2012.08.025. 
            Epub 2012 Aug 24.
  PUBMED    22939869
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gene.2012.08.025"; journalName: "Gene"; date: 
            "2012-11-10- 10"; volume: "509"; issue: "2"; pages: "215-22"
COMMENT     Sequence Label: pBABE (hygro) hYAP 1-2alpha
FEATURES             Location/Qualifiers
     source          1..6674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1513..1533)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1518..1847
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1698..1833
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1921..2946
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1921..2946
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     3463..3482
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             3744..4213
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(4328..5119,5120..5188))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4951..4970
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5189..5293)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5324..5793
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5858..6057
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             6258..6674
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6604..6626
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6642..6658
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 ggccggatcc cagtgtggtg gtacgtagga attcatggat cccgggcagc agccgccgcc
       61 tcaaccggcc ccccagggcc aagggcagcc gccttcgcag cccccgcagg ggcagggccc
      121 gccgtccgga cccgggcaac cggcacccgc ggcgacccag gcggcgccgc aggcaccccc
      181 cgccgggcat cagatcgtgc acgtccgcgg ggactcggag accgacctgg aggcgctctt
      241 caacgccgtc atgaacccca agacggccaa cgtgccccag accgtgccca tgaggctccg
      301 gaagctgccc gactccttct tcaagccgcc ggagcccaaa tcccactccc gacaggccag
      361 tactgatgca ggcactgcag gagccctgac tccacagcat gttcgagctc attcctctcc
      421 agcttctctg cagttgggag ctgtttctcc tgggacactg acccccactg gagtagtctc
      481 tggcccagca gctacaccca cagctcagca tcttcgacag tcttcttttg agatacctga
      541 tgatgtacct ctgccagcag gttgggagat ggcaaagaca tcttctggtc agagatactt
      601 cttaaatcac atcgatcaga caacaacatg gcaggacccc aggaaggcca tgctgtccca
      661 gatgaacgtc acagccccca ccagtccacc agtgcagcag aatatgatga actcggcttc
      721 aggtcctctt cctgatggat gggaacaagc catgactcag gatggagaaa tttactatat
      781 aaaccataag aacaagacca cctcttggct agacccaagg cttgaccctc gttttgccat
      841 gaaccagaga atcagtcaga gtgctccagt gaaacagcca ccacccctgg ctccccagag
      901 cccacaggga ggcgtcatgg gtggcagcaa ctccaaccag cagcaacaga tgcgactgca
      961 gcaactgcag atggagaagg agaggctgcg gctgaaacag caagaactgc ttcggcagga
     1021 gttagccctg cgtagccagt taccaacact ggagcaggat ggtgggactc aaaatccagt
     1081 gtcttctccc gggatgtctc aggaattgag aacaatgacg accaatagct cagatccttt
     1141 ccttaacagt ggcacctatc actctcgaga tgagagtaca gacagtggac taagcatgag
     1201 cagctacagt gtccctcgaa ccccagatga cttcctgaac agtgtggatg agatggatac
     1261 aggtgatact atcaaccaaa gcaccctgcc ctcacagcag aaccgtttcc cagactacct
     1321 tgaagccatt cctgggacaa atgtggacct tggaacactg gaaggagatg gaatgaacat
     1381 agaaggagag gagctgatgc caagtctgca ggaagctttg agttctgaca tccttaatga
     1441 catggagtct gttttggctg ccaccaagct agataaagaa agctttctta catggttata
     1501 ggtcgaccct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     1561 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     1621 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     1681 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     1741 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     1801 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttgccagct
     1861 ggggcgccct ctggtaaggt tgggaagccc tgcagatctg atcccggggg gcaatgagat
     1921 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     1981 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     2041 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     2101 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     2161 ggggaattta gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     2221 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     2281 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     2341 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     2401 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     2461 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     2521 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     2581 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     2641 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     2701 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     2761 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     2821 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     2881 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     2941 gaatagagta gatgccgacc gggatctatc gatgcggcgg gtgtggtggt tacgcgtaat
     3001 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3061 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3121 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3181 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3241 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3301 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3361 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3421 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3481 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3541 aggggggcgg agcctatgga aaaacgccag caacgcatcg ataaaataaa agattttatt
     3601 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta
     3661 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc
     3721 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     3781 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
     3841 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
     3901 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
     3961 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
     4021 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
     4081 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
     4141 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
     4201 cgggggtctt tcacatgcag catgtatcaa aattaatttg gttttttttc ttaagtattt
     4261 acattaaatg gccatagtgc ggccgcaaat caatctaaag tatatatgag taaacttggt
     4321 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4381 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4441 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4501 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4561 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4621 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4681 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4741 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4801 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4861 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4921 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4981 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5041 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5101 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5161 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5221 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5281 taggggttcc gcgcacattt ccccgaaaag tgccacctgc agcctgaata tgggccaaac
     5341 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
     5401 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     5461 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
     5521 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     5581 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     5641 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
     5701 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
     5761 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
     5821 cccctgccca gggaccaccg acccaccacc gggaggcaag ctggccagca acttatctgt
     5881 gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag
     5941 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga acacccggcc
     6001 gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg acctgaggaa
     6061 gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga gacgagaacc
     6121 taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga agccgcgcgt
     6181 cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt
     6241 atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc ttaggtcact
     6301 ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg
     6361 ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct
     6421 ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac
     6481 acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct
     6541 gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc
     6601 tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc
     6661 cttctctagg cgcc
//
