LOCUS       sequence-240717-        6572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression for funtional studies of cell growth promotion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Gaffney CJ, Oka T, Mazack V, Hilman D, Gat U, Muramatsu T, Inazawa 
            J, Golden A, Carey DJ, Farooq A, Tromp G, Sudol M
  TITLE     Identification, basic characterization and evolutionary analysis of 
            differentially spliced mRNA isoforms of human YAP1 gene.
  JOURNAL   Gene. 2012 Nov 10;509(2):215-22. doi: 10.1016/j.gene.2012.08.025. 
            Epub 2012 Aug 24.
  PUBMED    22939869
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gene.2012.08.025"; journalName: "Gene"; date: 
            "2012-11-10- 10"; volume: "509"; issue: "2"; pages: "215-22"
COMMENT     Sequence Label: pBABE (hygro) hYAP 1-1beta
FEATURES             Location/Qualifiers
     source          1..6572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1411..1431)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1416..1745
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1596..1731
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1819..2844
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1819..2844
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     3361..3380
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             3642..4111
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(4226..5017,5018..5086))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4849..4868
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5087..5191)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5222..5691
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5756..5955
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             6156..6572
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6502..6524
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6540..6556
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 ggccggatcc cagtgtggtg gtacgtagga attcatggat cccgggcagc agccgccgcc
       61 tcaaccggcc ccccagggcc aagggcagcc gccttcgcag cccccgcagg ggcagggccc
      121 gccgtccgga cccgggcaac cggcacccgc ggcgacccag gcggcgccgc aggcaccccc
      181 cgccgggcat cagatcgtgc acgtccgcgg ggactcggag accgacctgg aggcgctctt
      241 caacgccgtc atgaacccca agacggccaa cgtgccccag accgtgccca tgaggctccg
      301 gaagctgccc gactccttct tcaagccgcc ggagcccaaa tcccactccc gacaggccag
      361 tactgatgca ggcactgcag gagccctgac tccacagcat gttcgagctc attcctctcc
      421 agcttctctg cagttgggag ctgtttctcc tgggacactg acccccactg gagtagtctc
      481 tggcccagca gctacaccca cagctcagca tcttcgacag tcttcttttg agatacctga
      541 tgatgtacct ctgccagcag gttgggagat ggcaaagaca tcttctggtc agagatactt
      601 cttaaatcac atcgatcaga caacaacatg gcaggacccc aggaaggcca tgctgtccca
      661 gatgaacgtc acagccccca ccagtccacc agtgcagcag aatatgatga actcggcttc
      721 agccatgaac cagagaatca gtcagagtgc tccagtgaaa cagccaccac ccctggctcc
      781 ccagagccca cagggaggcg tcatgggtgg cagcaactcc aaccagcagc aacagatgcg
      841 actgcagcaa ctgcagatgg agaaggagag gctgcggctg aaacagcaag aactgcttcg
      901 gcaggtgagg ccacaggagt tagccctgcg tagccagtta ccaacactgg agcaggatgg
      961 tgggactcaa aatccagtgt cttctcccgg gatgtctcag gaattgagaa caatgacgac
     1021 caatagctca gatcctttcc ttaacagtgg cacctatcac tctcgagatg agagtacaga
     1081 cagtggacta agcatgagca gctacagtgt ccctcgaacc ccagatgact tcctgaacag
     1141 tgtggatgag atggatacag gtgatactat caaccaaagc accctgccct cacagcagaa
     1201 ccgtttccca gactaccttg aagccattcc tgggacaaat gtggaccttg gaacactgga
     1261 aggagatgga atgaacatag aaggagagga gctgatgcca agtctgcagg aagctttgag
     1321 ttctgacatc cttaatgaca tggagtctgt tttggctgcc accaagctag ataaagaaag
     1381 ctttcttaca tggttatagg tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt
     1441 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     1501 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     1561 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     1621 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     1681 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     1741 gcaaaaagct tgccagctgg ggcgccctct ggtaaggttg ggaagccctg cagatctgat
     1801 cccggggggc aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     1861 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     1921 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     1981 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     2041 ccggaagtgc ttgacattgg ggaatttagc gagagcctga cctattgcat ctcccgccgt
     2101 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     2161 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     2221 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     2281 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     2341 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     2401 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     2461 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     2521 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     2581 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     2641 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     2701 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     2761 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     2821 actcgtccga gggcaaagga atagagtaga tgccgaccgg gatctatcga tgcggcgggt
     2881 gtggtggtta cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2941 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3001 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3061 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3121 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3181 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3241 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3301 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3361 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3421 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcatcgat
     3481 aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
     3541 gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag
     3601 aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag
     3661 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga
     3721 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
     3781 agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc
     3841 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc
     3901 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct
     3961 cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa
     4021 ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag
     4081 tgattgacta cccgtcagcg ggggtctttc acatgcagca tgtatcaaaa ttaatttggt
     4141 tttttttctt aagtatttac attaaatggc catagtgcgg ccgcaaatca atctaaagta
     4201 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4261 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4321 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4381 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4441 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4501 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4561 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4621 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4681 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4741 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4801 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4861 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4921 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4981 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5041 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5101 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5161 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcag
     5221 cctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     5281 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     5341 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta
     5401 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
     5461 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
     5521 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg
     5581 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
     5641 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc
     5701 tcgtccggga tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggcaagct
     5761 ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc
     5821 tgcgtcggta ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg
     5881 agttctgaac acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt
     5941 gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc
     6001 tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt
     6061 ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct
     6121 gtctgactgt gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa
     6181 gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg
     6241 tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat
     6301 ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt
     6361 cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg
     6421 cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc
     6481 ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc
     6541 tttatccagc cctcactcct tctctaggcg cc
//
