LOCUS       sequence-240720-        3132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Negative regulator of Fus3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS509
FEATURES             Location/Qualifiers
     source          1..3132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1499..1533)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1604..2263)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2178..2197
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2445..3033
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2934..2953
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc ctatgcaatt tcactcagat aagcagcatt tggacagtaa aaccgacatc
       61 gatttcaagc caaactcacc gcgttcctta caaaatagga ataccaaaaa tttatcttta
      121 gatatagcag cactccatcc attaatggaa ttctcatcgc caagccaaga tgtgccaggt
      181 tcagtaaaat tcccatcgcc gacacctttg aatctattta tgaagcccaa acctattgtg
      241 ttggagaaat gtccaccaaa agtaagtcca aggccaacgc caccatcgct gtcgatgagg
      301 cgaagcgagg cctctatata cacactacca acatctttga agaaccgaac tgtttctcca
      361 agcgtgtata caaaatcatc cacagtatcg tctatcagta agctgtcatc atcatcaccg
      421 ttatcgtcat tttcagaaaa acctcatctg aatagagtcc attcattatc cgtgaaaact
      481 aaagacttga agttgaaggg aattagagga cgttctcaaa cgatctcagg gttagagaca
      541 tccacgccaa tttctagtac tcgtgaaggt actttagata gtacggatgt caacagattt
      601 tctaaccaaa agaatatgca aacaacattg attttcccgg aggaggactc ggatctgaat
      661 attgatatgg tgcatgcaga gatttatcaa cgaacggttt atttagatgg accattgctg
      721 gtactgccgc ctaatttgta tctatattca gaacccaaac tagaagatat attatcgttt
      781 gatttagtca ttaatgttgc caaagaaata ccgaacctgg agtttttaat accgccggaa
      841 atggcacata aaataaaata ttaccatatt gaatggacac acacatccaa gatcgtcaag
      901 gacttatccc gattgacacg cattatacat accgctcatt cgcaaggcaa gaaaatactc
      961 gtacactgtc agtgtggagt atcaagatcg gcgtcattga ttgtggcgta tatcatgcga
     1021 tattatggct tgagtttaaa tgatgcatac aataagctga aaggtgttgc taaggatata
     1081 agtccaaaca tgggtctgat cttccaactt atggaatggg gaaccatgtt gtccaagaac
     1141 tcaccgggcg aagaaggaga gactgttcac atgcctgagg aagatgacat cggaaacaac
     1201 gaagtttcct cgactacgaa gtcctactct tctgcgtcct ttagaagttt tcccatggta
     1261 acgaatctat catcgtcgcc gaatgacagt tctgtcaatt cttcggaagt aacgccaaga
     1321 actcctgcta cgttgactgg agcaaggacc gcactggcca cagaacgcgg ggaagatgat
     1381 gagcactgta aaagtttgtc tcaacccgca gattcactgg aagcttctgt ggacaacgaa
     1441 tcaatatcta ctgccccgga acagatgatg tttcttcctg gatcctgaga ccagaccaat
     1501 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     1561 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
     1621 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
     1681 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     1741 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     1801 tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag
     1861 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     1921 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     1981 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     2041 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     2101 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     2161 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     2221 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     2281 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     2341 agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg
     2401 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2461 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2521 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2581 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2641 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2701 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2761 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2821 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2881 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2941 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3001 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3061 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3121 gtggataacc gt
//
