LOCUS       sequence-240721-        3021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Negative regulator of Ste12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS510
FEATURES             Location/Qualifiers
     source          1..3021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1388..1422)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1493..2152)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2067..2086
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2334..2922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2823..2842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc ctatggccgt atcagcccgt ttgagaacga ctgccgagga tacatccatt
       61 gctaaatcaa cacaagatcc aattggtgat acagaaatca gtgtagcaaa tgctaagggc
      121 agcagcgata gcaacattaa gaattcgcca ggcggaaaca gcgttggtca ggagtcggag
      181 ctagagcatg tccctgagga ggatgactct ggtgacaagg aagcagatca tgaggattct
      241 gagacagcca ctgcgaagaa gaggaaagcc caaccattga agaatccgaa gaaatcattg
      301 aagaggggca gagtcccggc gcctttgaat ttatcggata gcaacactaa tacacacggt
      361 ggtaatatta aggacggcaa cttggcttcg tctaactctg cacattttcc tcctgttgct
      421 aatcaaaacg tgaaaagcgc gcccgcacaa gttactcagc attccaagtt ccagccccgt
      481 gtccaatact tgggaaaggc cagttctaga caatctatac aagtgaataa tagcagcaat
      541 agttatggga aaccacacat gccctcggcg ggcatcatga gcgccatgaa cccttacatg
      601 cccatgaatc gctacataat gtcaccatat tataatccgt acggtatccc tccacctcac
      661 atgctgaaca agcccataat gacaccctac gtgtcgtatc catatccaat gggaccgcgg
      721 acctccattc cttatgctat gcaaggtggc aacgctaggc cctacgaaga aaatgagtat
      781 agtgctagca attacagaaa caagagggtt aacgactcat atgattcgcc tttgagtggc
      841 accgcttcca ctgggaaaac cagacgatcc gaggaaggct caagaaattc tagcgtagga
      901 tcaagtgcga atgccggtcc tacgcaacag cgcgcggatc tacgcccagc agatatgata
      961 cctgctgaag aataccactt tgaacgggat gcattactct cggccaacac gaaagccaga
     1021 agcgcaagca caagcacaag tacaagcaca agcacaaacc gggacaggag ttcatggcat
     1081 gaggcagaac ccaataaaga cgaagaagag ggtacggact tggccatcga ggacggagcg
     1141 gtccccactc ccacttttac tacgttccag cggacctcgc agccgcagca gcaatcacct
     1201 agtcttcttc aaggcgaaat ccgactctca tcgcatattt ttgccttcga gttccccctg
     1261 agctctagca atgtagacaa gaaaatgttt atgagcatat gtaataaagt atggaatgaa
     1321 tcaaaggagc tgacaaaaaa atcatcatca catcacagaa ccggaaaagg atcctgagac
     1381 cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     1441 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
     1501 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     1561 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     1621 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     1681 aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa
     1741 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     1801 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     1861 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     1921 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     1981 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     2041 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     2101 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     2161 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     2221 tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt
     2281 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2341 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2401 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2461 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2521 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2581 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2641 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2701 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2761 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2821 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2881 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2941 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3001 cctgattctg tggataaccg t
//
