LOCUS       sequence-240722-        2634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Negative regulator of Ste12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS511
FEATURES             Location/Qualifiers
     source          1..2634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1001..1035)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1106..1765)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1680..1699
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1947..2535
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2436..2455
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc ctatgaataa agaagagcaa gaagacccac agcaagagca aatatcaact
       61 gttcaggaaa atgatcccag gaatttgcag caactgggaa tgctgttagt atctccaggg
      121 cttgatgaag acagattgag tgagaaaatg atttcgaaga tcaaaaaatc cagggatatc
      181 gaaaaaaatc aaaaattgct catatctagg ctgtcgcaaa aagaggaaga tcatagtggt
      241 aagcctccta ccattacgac ttctccagca gagaaaaccg taccctttaa gtcgctgaat
      301 cattctttaa agaggaaaag ggtacctcca gcgctaaatt tttccgatat acaagcatct
      361 tctcatttgc atggatctaa aagtgctcca ccaaacataa caagatttcc gcagcacaaa
      421 aatagcctta gggtcagata tatgggtagg atggctccta cgaatcaaga ttatcatcct
      481 tcagtggcca attcatatat gacagcaacc tacccttatc catatactgg actgccacca
      541 gtaccatgct acccatattc ttcaactcca acacaaacgc acgcatacga aggctattat
      601 tccccgatgt atcccggccc cttgtataat aatggtataa taccagccga ctaccatgca
      661 aagaggaaga agttggctgg tagatcacca cacttggaag atttgacatc gagaaaaaga
      721 acctttgtct ccaaacacca caacggagat ccaatcataa gtaagactga tgaagacatt
      781 gaatgctctg tcacgaaaaa ttcattaagt gagggcgctt cacttaacga cgatgccgat
      841 gatgacaacg acaaagaaag gatcattatt ggagaaatct ctctgtatga tgatgttttc
      901 aaatttgaag ttcgcgacga caaaaatgac tatatgaaag catgtgaaac aatctggact
      961 gaatggcata acttgaagaa aggatcctga gaccagacca ataaaaaacg cccggcggca
     1021 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
     1081 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
     1141 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     1201 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     1261 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     1321 gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     1381 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     1441 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     1501 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
     1561 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     1621 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     1681 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     1741 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     1801 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     1861 gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1921 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1981 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2041 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2101 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2161 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2221 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2281 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2341 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2401 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2461 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2521 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2581 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgt
//
