LOCUS       sequence-240729-        6549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression for funtional studies of cell growth promotion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Gaffney CJ, Oka T, Mazack V, Hilman D, Gat U, Muramatsu T, Inazawa 
            J, Golden A, Carey DJ, Farooq A, Tromp G, Sudol M
  TITLE     Identification, basic characterization and evolutionary analysis of 
            differentially spliced mRNA isoforms of human YAP1 gene.
  JOURNAL   Gene. 2012 Nov 10;509(2):215-22. doi: 10.1016/j.gene.2012.08.025. 
            Epub 2012 Aug 24.
  PUBMED    22939869
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gene.2012.08.025"; journalName: "Gene"; date: 
            "2012-11-10- 10"; volume: "509"; issue: "2"; pages: "215-22"
COMMENT     Sequence Label: pBABE (hygro) hYAP 1-1alpha
FEATURES             Location/Qualifiers
     source          1..6549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     347..369
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     385..401
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(1816..1836)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1821..2150
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2001..2136
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2224..3249
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2224..3249
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     3755..3774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4036..4505
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(4620..5411,5412..5480))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5243..5262
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5481..5585)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5616..6085
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    6150..6349
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
       61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
      241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccggc
      421 cggatcccag tgtggtggta cgtaggaatt catggatccc gggcagcagc cgccgcctca
      481 accggccccc cagggccaag ggcagccgcc ttcgcagccc ccgcaggggc agggcccgcc
      541 gtccggaccc gggcaaccgg cacccgcggc gacccaggcg gcgccgcagg caccccccgc
      601 cgggcatcag atcgtgcacg tccgcgggga ctcggagacc gacctggagg cgctcttcaa
      661 cgccgtcatg aaccccaaga cggccaacgt gccccagacc gtgcccatga ggctccggaa
      721 gctgcccgac tccttcttca agccgccgga gcccaaatcc cactcccgac aggccagtac
      781 tgatgcaggc actgcaggag ccctgactcc acagcatgtt cgagctcatt cctctccagc
      841 ttctctgcag ttgggagctg tttctcctgg gacactgacc cccactggag tagtctctgg
      901 cccagcagct acacccacag ctcagcatct tcgacagtct tcttttgaga tacctgatga
      961 tgtacctctg ccagcaggtt gggagatggc aaagacatct tctggtcaga gatacttctt
     1021 aaatcacatc gatcagacaa caacatggca ggaccccagg aaggccatgc tgtcccagat
     1081 gaacgtcaca gcccccacca gtccaccagt gcagcagaat atgatgaact cggcttcagc
     1141 catgaaccag agaatcagtc agagtgctcc agtgaaacag ccaccacccc tggctcccca
     1201 gagcccacag ggaggcgtca tgggtggcag caactccaac cagcagcaac agatgcgact
     1261 gcagcaactg cagatggaga aggagaggct gcggctgaaa cagcaagaac tgcttcggca
     1321 ggagttagcc ctgcgtagcc agttaccaac actggagcag gatggtggga ctcaaaatcc
     1381 agtgtcttct cccgggatgt ctcaggaatt gagaacaatg acgaccaata gctcagatcc
     1441 tttccttaac agtggcacct atcactctcg agatgagagt acagacagtg gactaagcat
     1501 gagcagctac agtgtccctc gaaccccaga tgacttcctg aacagtgtgg atgagatgga
     1561 tacaggtgat actatcaacc aaagcaccct gccctcacag cagaaccgtt tcccagacta
     1621 ccttgaagcc attcctggga caaatgtgga ccttggaaca ctggaaggag atggaatgaa
     1681 catagaagga gaggagctga tgccaagtct gcaggaagct ttgagttctg acatccttaa
     1741 tgacatggag tctgttttgg ctgccaccaa gctagataaa gaaagctttc ttacatggtt
     1801 ataggtcgac cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     1861 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     1921 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     1981 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2041 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     2101 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttgcca
     2161 gctggggcgc cctctggtaa ggttgggaag ccctgcagat ctgatcccgg ggggcaatga
     2221 gatatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     2281 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     2341 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     2401 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     2461 attggggaat ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     2521 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     2581 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     2641 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     2701 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     2761 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     2821 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     2881 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     2941 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     3001 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     3061 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     3121 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     3181 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     3241 aaggaataga gtagatgccg accgggatct atcgatgcgg cgggtgtggt ggttacgcgt
     3301 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3361 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3421 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag cacctcgctc
     3481 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3541 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3601 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3661 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3721 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3781 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3841 ggagcctatg gaaaaacgcc agcaacgcat cgataaaata aaagatttta tttagtctcc
     3901 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     3961 cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag
     4021 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     4081 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
     4141 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc
     4201 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     4261 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
     4321 ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg
     4381 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
     4441 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
     4501 tttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa
     4561 tggccatagt gcggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     4621 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4681 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4741 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4801 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4861 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4921 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4981 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     5041 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     5101 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     5161 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     5221 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     5281 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     5341 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     5401 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     5461 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     5521 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     5581 ccgcgcacat ttccccgaaa agtgccacct gcagcctgaa tatgggccaa acaggatatc
     5641 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg
     5701 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     5761 tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
     5821 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
     5881 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
     5941 gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct
     6001 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
     6061 actacccgtc agcgggggtc tttcatttgg gggctcgtcc gggatcggga gacccctgcc
     6121 cagggaccac cgacccacca ccgggaggca agctggccag caacttatct gtgtctgtcc
     6181 gattgtctag tgtctatgac tgattttatg cgcctgcgtc ggtactagtt agctaactag
     6241 ctctgtatct ggcggacccg tggtggaact gacgagttct gaacacccgg ccgcaaccct
     6301 gggagacgtc ccagggactt tgggggccgt ttttgtggcc cgacctgagg aagggagtcg
     6361 atgtggaatc cgaccccgtc aggatatgtg gttctggtag gagacgagaa cctaaaacag
     6421 ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc gaagccgcgc gtcttgtctg
     6481 ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct
     6541 gaaaattag
//
