LOCUS       sequence-240732-        6608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression for funtional studies of cell growth promotion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Gaffney CJ, Oka T, Mazack V, Hilman D, Gat U, Muramatsu T, Inazawa 
            J, Golden A, Carey DJ, Farooq A, Tromp G, Sudol M
  TITLE     Identification, basic characterization and evolutionary analysis of 
            differentially spliced mRNA isoforms of human YAP1 gene.
  JOURNAL   Gene. 2012 Nov 10;509(2):215-22. doi: 10.1016/j.gene.2012.08.025. 
            Epub 2012 Aug 24.
  PUBMED    22939869
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gene.2012.08.025"; journalName: "Gene"; date: 
            "2012-11-10- 10"; volume: "509"; issue: "2"; pages: "215-22"
COMMENT     Sequence Label: pBABE (hygro) hYAP 1-1gamma
FEATURES             Location/Qualifiers
     source          1..6608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     347..369
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     385..401
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(1864..1884)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1869..2198
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2049..2184
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2272..3297
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2272..3297
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     3814..3833
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4095..4564
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(4679..5470,5471..5539))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5302..5321
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5540..5644)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5675..6144
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    6209..6408
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
       61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
      241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccggc
      421 cggatcccag tgtggtggta cgtaggaatt catggatccc gggcagcagc cgccgcctca
      481 accggccccc cagggccaag ggcagccgcc ttcgcagccc ccgcaggggc agggcccgcc
      541 gtccggaccc gggcaaccgg cacccgcggc gacccaggcg gcgccgcagg caccccccgc
      601 cgggcatcag atcgtgcacg tccgcgggga ctcggagacc gacctggagg cgctcttcaa
      661 cgccgtcatg aaccccaaga cggccaacgt gccccagacc gtgcccatga ggctccggaa
      721 gctgcccgac tccttcttca agccgccgga gcccaaatcc cactcccgac aggccagtac
      781 tgatgcaggc actgcaggag ccctgactcc acagcatgtt cgagctcatt cctctccagc
      841 ttctctgcag ttgggagctg tttctcctgg gacactgacc cccactggag tagtctctgg
      901 cccagcagct acacccacag ctcagcatct tcgacagtct tcttttgaga tacctgatga
      961 tgtacctctg ccagcaggtt gggagatggc aaagacatct tctggtcaga gatacttctt
     1021 aaatcacatc gatcagacaa caacatggca ggaccccagg aaggccatgc tgtcccagat
     1081 gaacgtcaca gcccccacca gtccaccagt gcagcagaat atgatgaact cggcttcagc
     1141 catgaaccag agaatcagtc agagtgctcc agtgaaacag ccaccacccc tggctcccca
     1201 gagcccacag ggaggcgtca tgggtggcag caactccaac cagcagcaac agatgcgact
     1261 gcagcaactg cagatggaga aggagaggct gcggctgaaa cagcaagaac tgcttcggca
     1321 ggcaatgcgg aatatcaatc ccagcacagc aaattctcca aaatgtcagg agttagccct
     1381 gcgtagccag ttaccaacac tggagcagga tggtgggact caaaatccag tgtcttctcc
     1441 cgggatgtct caggaattga gaacaatgac gaccaatagc tcagatcctt tccttaacag
     1501 tggcacctat cactctcgag atgagagtac agacagtgga ctaagcatga gcagctacag
     1561 tgtccctcga accccagatg acttcctgaa cagtgtggat gagatggata caggtgatac
     1621 tatcaaccaa agcaccctgc cctcacagca gaaccgtttc ccagactacc ttgaagccat
     1681 tcctgggaca aatgtggacc ttggaacact ggaaggagat ggaatgaaca tagaaggaga
     1741 ggagctgatg ccaagtctgc aggaagcttt gagttctgac atccttaatg acatggagtc
     1801 tgttttggct gccaccaagc tagataaaga aagctttctt acatggttat aggtcgaccc
     1861 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     1921 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     1981 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     2041 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     2101 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     2161 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttgccagc tggggcgccc
     2221 tctggtaagg ttgggaagcc ctgcagatct gatcccgggg ggcaatgaga tatgaaaaag
     2281 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     2341 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     2401 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     2461 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattt
     2521 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     2581 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     2641 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     2701 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     2761 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     2821 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     2881 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     2941 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     3001 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     3061 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     3121 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     3181 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     3241 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagagt
     3301 agatgccgac cgggatctat cgatgcggcg ggtgtggtgg ttacgcgtaa tctgctgctt
     3361 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3421 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3481 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3541 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3601 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3661 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3721 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3781 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3841 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3901 gagcctatgg aaaaacgcca gcaacgcatc gataaaataa aagattttat ttagtctcca
     3961 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     4021 attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg
     4081 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     4141 cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     4201 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc
     4261 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     4321 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     4381 tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
     4441 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     4501 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     4561 ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat
     4621 ggccatagtg cggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4681 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4741 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4801 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4861 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4921 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4981 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5041 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5101 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5161 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5221 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5281 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5341 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5401 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5461 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5521 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5581 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5641 cgcgcacatt tccccgaaaa gtgccacctg cagcctgaat atgggccaaa caggatatct
     5701 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg
     5761 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
     5821 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
     5881 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     5941 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     6001 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
     6061 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     6121 ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc
     6181 agggaccacc gacccaccac cgggaggcaa gctggccagc aacttatctg tgtctgtccg
     6241 attgtctagt gtctatgact gattttatgc gcctgcgtcg gtactagtta gctaactagc
     6301 tctgtatctg gcggacccgt ggtggaactg acgagttctg aacacccggc cgcaaccctg
     6361 ggagacgtcc cagggacttt gggggccgtt tttgtggccc gacctgagga agggagtcga
     6421 tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg agacgagaac ctaaaacagt
     6481 tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc
     6541 tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg
     6601 aaaattag
//
