LOCUS       sequence-240733-        4717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast optimized 
            mTagBFP2 with SpHis5 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Lee S, Lim WA, Thorn KS
  TITLE     Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors 
            for S. cerevisiae.
  JOURNAL   PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902.
            Print 2013.
  PUBMED    23844123
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-2"; volume: "8(7):e67902. doi"; pages: " 
            10.1371/journal.pone.0067902. Print 2013"
COMMENT     Sequence Label: pFA6a-link-yomTagBFP2-SpHis5
FEATURES             Location/Qualifiers
     source          1..4717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     30..49
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        130..148
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     130..147
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      934..1121
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(989..1013)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            1196..2396
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5 
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        1196..1539
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1540..2193
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHTNQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMSNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     terminator      2199..2396
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2500..2519)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2501..2519)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2606..2623)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2777..3365)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2857..2876)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3536..4327,4328..4396))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4159..4178
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4397..4501)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4569..4587
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4625..4647)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
       61 ccatatggac atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat
      121 acacatacga tttaggtgac actatagaac gcggccgcca gctgaagctt cgtacgctgc
      181 aggtcgacgg atcggtgacg gtgctggttt aattaacatg agtgagttga ttaaggaaaa
      241 catgcacatg aagttgtaca tggaaggaac agtagataat catcatttca aatgtacgtc
      301 agaaggtgag ggcaaacctt atgaaggaac tcaaactatg agaattaaag tcgttgaggg
      361 tggcccacta ccatttgcat tcgatatcct cgcaacatct tttctgtatg gttctaaaac
      421 ctttatcaac catacacaag gcataccaga tttctttaag caatcatttc ctgaaggttt
      481 cacatgggaa agagtaacta cctatgaaga tggtggtgtc cttacagcta ctcaagacac
      541 gtctttgcag gatggttgtt taatctacaa tgttaagata agaggagtga actttacatc
      601 caatggacca gtcatgcaaa agaaaactct aggttgggag gcattcacag aaactttgta
      661 cccagccgat gggggcttag aagggagaaa tgacatggct ttaaaactgg ttggcggtag
      721 ccatttgatt gcgaacgcta aaacaaccta cagaagcaaa aagccagcta aaaatcttaa
      781 gatgcctgta tactacgtgg attacagatt agaaaggaag gaagctaata atgaaactta
      841 cgttgaacag cacgaggtgg ccgttgcccg ttattgcgac cttccttcaa aattgggaca
      901 caaactaaac taaggcgcgc cacttctaaa taagcgaatt tcttatgatt tatgattttt
      961 attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt
     1021 ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag
     1081 gtatagtatg aggtcgctct tattgaccac acctctaccg gcagatccgc tagggataac
     1141 agggtaatat agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
     1201 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
     1261 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
     1321 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg
     1381 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
     1441 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
     1501 aggatacagt tctcacatca catccgaaca taaacaacca tgggtaggag ggcttttgta
     1561 gaaagaaata cgaacgaaac gaaaatcagc gttgccatcg ctttggacaa agctccctta
     1621 cctgaagagt cgaattttat tgatgaactt ataacttcca agcatacaaa ccaaaaggga
     1681 gaacaagtaa tccaagtaga cacgggaatt ggattcttgg atcacatgta tcatgcactg
     1741 gctaaacatg caggctggag cttacgactt tactcaagag gtgatttaat catcgatgat
     1801 catcacactg cagaagatac tgctattgca cttggtattg cattcaagca ggctatgagt
     1861 aactttgccg gcgttaaaag atttggacat gcttattgtc cacttgacga agctctttct
     1921 agaagcgtag ttgacttgtc gggacggccc tatgctgtta tcgatttggg attaaagcgt
     1981 gaaaaggttg gggaattgtc ctgtgaaatg atccctcact tactatattc cttttcggta
     2041 gcagctggaa ttactttgca tgttacctgc ttatatggta gtaatgacca tcatcgtgct
     2101 gaaagcgctt ttaaatctct ggctgttgcc atgcgcgcgg ctactagtct tactggaagt
     2161 tctgaagtcc caagcacgaa gggagtgttg taaagagtac tgacaataaa aagattcttg
     2221 ttttcaagaa cttgtcattt gtatagtttt tttatattgt agttgttcta ttttaatcaa
     2281 atgttagcgt gatttatatt ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa
     2341 gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt
     2401 cgattcgata ctaacgccgc catccagttt aaacgagctc gaattcatcg atgatatcag
     2461 atccactagt ggcctatgcg gccgcggatc tgccggtctc cctatagtga gtcgtattaa
     2521 tttcgataag ccaggttaac ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2581 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2641 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2701 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2761 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2821 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2881 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2941 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3001 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3061 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3121 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3181 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3241 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3301 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3361 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3421 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3481 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3541 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3601 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3661 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3721 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3781 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3841 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3901 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3961 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4021 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4081 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4141 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4201 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4261 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4321 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4381 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4441 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4501 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     4561 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     4621 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     4681 ggagcagaca agcccgtcag ggcgcgtcag cgggtgt
//
