LOCUS       sequence-240738-       11551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of Bach1 S990A mutant (BRCA binding mutant)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11551)
  TITLE     Cantor Lab plasmids
REFERENCE   2  (bases 1 to 11551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOZ-FH-C Bach1 S990A
FEATURES             Location/Qualifiers
     source          1..11551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(291..308)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(291..307)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(300..322)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(516..535)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     635..657
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(695..713)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        781..885
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(886..954,955..1746)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1104..1123)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1917..2505
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2406..2425
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2659..2676
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2793..2814
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2829..2834,2835..2852,2853..2859)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2867..2883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2872..2894
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2891..2907
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2891..2907
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    3892..4249
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             4314..4730
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     4660..4682
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4698..4714
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    4740..5114
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     5052..5068
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             8901..8924
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             8940..8966
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             8940..8966
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    9039..9588
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9183..9200)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9410..9429
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             10679..11148
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 ttgattgatg tgtgtgtgtg tgattgtgtt tgtgtgtgtg attgtgtata tgtgtgtatg
       61 gttgtgtgtg attgtgtgta tgtatgtttg tgtgtgattg tgtgtgtgtg attgtgcatg
      121 tgtgtgtgtg tgattgtgtt tatgtgtatg attgtgtgtg tgtgtgtgtg tgtgtgtgtg
      181 tgtgtgtgtg tgtgtgttgt gtatatatat ttatggtagt gagaggcaac gctccggctc
      241 aggtgtcagg ttggtttttg agacagagtc tttcacttag cttggaattc actggccgtc
      301 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      361 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      421 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
      481 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
      541 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
      601 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
      661 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
      721 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
      781 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      841 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      901 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      961 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     1021 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     1081 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     1141 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     1201 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1261 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1321 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     1381 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     1441 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     1501 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     1561 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     1621 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     1681 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     1741 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1801 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1861 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1921 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1981 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2041 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2101 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2161 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2221 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2281 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2341 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2401 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2461 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2521 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2581 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2641 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     2701 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     2761 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2821 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2881 caatttcaca caggaaacag ctatgaccat gattacgcca agctttgctc ttaggagttt
     2941 cctaatacat cccaaactca aatatataaa gcatttgact tgttctatgc cctagggggc
     3001 ggggggaagc taagccagct ttttttaaca tttaaaatgt taattccatt ttaaatgcac
     3061 agatgttttt atttcataag ggtttcaatg tgcatgaatg ctgcaatatt cctgttacca
     3121 aagctagtat aaataaaaat agataaacgt ggaaattact tagagtttct gtcattaacg
     3181 tttccttcct cagttgacaa cataaatgcg ctgctgagaa gccagtttgc atctgtcagg
     3241 atcaatttcc cattatgcca gtcatattaa ttactagtca attagttgat ttttattttt
     3301 gacatataca tgtgaaagtc cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     3361 tttgcaaggc atggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa
     3421 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     3481 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
     3541 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     3601 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     3661 agagcccaca acccctcact cggcgcgcca gtcctccgat tgactgagtc gcccgggtac
     3721 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     3781 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt
     3841 ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
     3901 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg
     3961 tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
     4021 cggaacaccc ggccgcaacc ctgggagacg tcccagggac ttcgggggcc gtttttgtgg
     4081 cccgacctga gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc cccttagagg
     4141 agggatatgt ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat
     4201 ttttgctttc ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc
     4261 tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaatatgggc ccgggccaga
     4321 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc
     4381 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc
     4441 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg
     4501 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg
     4561 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
     4621 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
     4681 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc cccatatggc
     4741 catatgagat cctatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga
     4801 caagagttac taacagcccc tctctccaag ctcacttaca ggctctctac ttagtccagc
     4861 acgaagtctg gagacctctg gcggcagcct accaagaaca actggaccga ccggtggtac
     4921 ctcaccctta ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag
     4981 aacctcgctg gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag
     5041 acggcatcgc agcttggata cacgccgccc acgtgaaggc tgccgacccc gggggtggac
     5101 catcctctag actgccagat ctttagctcg aggccaccat gtcttcaatg tggtctgaat
     5161 atacaattgg tggggtgaag atttactttc cttataaagc ttacccgtca cagcttgcta
     5221 tgatgaattc tattctcaga ggattaaaca gcaagcaaca ttgtttgttg gagagtccca
     5281 caggaagtgg aaaaagctta gccttacttt gttctgcttt agcatggcaa caatctctta
     5341 gtgggaaacc agcagatgag ggcgtaagtg aaaaagctga agtacaattg tcatgttgtt
     5401 gtgcatgcca ttcaaaggat tttacaaaca atgacatgaa ccaaggaact tcacgtcatt
     5461 tcaactatcc aagcacacca ccttctgaaa gaaatggcac ttcatcaact tgtcaagact
     5521 cccctgaaaa aaccactctg gctgcaaagt tatctgctaa gaaacaggca tccatataca
     5581 gagatgaaaa tgatgatttt caagtagaga agaaaagaat tcgaccctta gaaactacac
     5641 agcagattag aaaacgtcat tgctttggaa cagaagtaca caatttggat gcaaaagttg
     5701 attcaggaaa gactgtaaaa ctcaactctc cactggaaaa gataaactcc ttttcgccac
     5761 agaaaccccc tggccactgt tctaggtgct gttgttctac taaacaagga aacagtcaag
     5821 agtcatcgaa taccattaag aaggatcata cagggaaatc caagataccc aaaatatatt
     5881 ttgggacacg cacacacaag cagattgctc agattactag agagctccgg aggacggcat
     5941 attcaggggt tccaatgact attctttcca gcagggatca tacttgtgtc catcctgagg
     6001 tagtcggtaa cttcaacaga aatgagaagt gcatggaatt gctagatggg aaaaacggaa
     6061 aatcctgcta tttttatcat ggagttcata aaattagtga tcagcacaca ttacagactt
     6121 tccaagggat gtgcaaagcc tgggatatag aagaacttgt cagcctgggg aagaaactaa
     6181 aggcctgtcc atattacaca gcccgagaac taatacaaga tgctgacatc atattttgtc
     6241 cctacaacta tcttctagat gcacaaataa gggaaagtat ggatttaaat ctgaaagaac
     6301 aggttgtcat tttagatgaa gctcataaca tcgaggactg tgctcgggaa tcagcaagtt
     6361 acagtgtaac agaagttcag cttcggtttg ctcgggatga actagatagt atggtcaaca
     6421 ataatataag gaagaaagat catgaacccc tacgagctgt gtgctgtagc ctcattaatt
     6481 ggttagaagc aaacgctgaa tatcttgtag aaagagatta tgaatcagct tgtaaaatat
     6541 ggagtggaaa tgaaatgctc ttaactttac acaaaatggg tatcaccact gctacttttc
     6601 ccattttgca gggacatttt tctgctgttc ttcaaaaaga ggaaaaaatc tcaccaattt
     6661 atggtaaaga ggaggcaaga gaagtacctg ttattagtgc atcaactcaa ataatgctta
     6721 aaggactttt tatggtactt gactatcttt ttaggcaaaa tagcagattt gcagatgatt
     6781 ataaaattgc gattcaacag acttactcct ggacaaatca gattgatatt tcagacaaaa
     6841 atgggttgtt ggttctacca aaaaataaga aacgttcacg acagaaaact gcagttcatg
     6901 tgctaaactt ttggtgctta aatccagctg tggccttttc agatattaat ggcaaagttc
     6961 agaccattgt tttgacatct ggtacattat caccaatgaa atccttttcg tcagaacttg
     7021 gtgttacatt tactatccag ctggaggcta atcatatcat taaaaattca caggtttggg
     7081 ttggtaccat tgggtcaggc cccaagggtc ggaatctctg tgctaccttc cagaatactg
     7141 aaacatttga gttccaagat gaagtgggag cacttttgtt atctgtgtgc cagactgtga
     7201 gccaaggaat tttgtgtttc ttgccatctt acaagttatt agaaaaatta aaagaacgtt
     7261 ggctctctac tggtttatgg cataatctgg agttggtgaa gacagtcatt gtagaaccac
     7321 agggaggaga aaaaacaaat tttgatgaat tactgcaggt gtactatgac gcaatcaaat
     7381 acaaaggaga gaaagatgga gctctcctgg tagcagtttg tcgtggtaaa gtgagtgagg
     7441 gtctggattt ctcagatgac aatgcccgtg ctgtcataac aataggaatt ccttttccaa
     7501 atgtgaaaga tctacaggtt gaactaaaac gacaatacaa tgaccaccat tcaaaattga
     7561 gaggtcttct acctggccgt cagtggtatg aaattcaagc atacagggcc ttaaaccagg
     7621 cccttggtag atgtattaga cacagaaatg attggggagc tcttattcta gtggatgatc
     7681 gctttaggaa taacccaagt cgctatatat ctggactttc taaatgggta cggcagcaga
     7741 ttcagcacca ttcaaccttt gaaagtgcac tggagtcctt ggctgaattt tccaaaaagc
     7801 atcaaaaagt tcttaatgta tccataaagg acagaaccaa tatacaggac aatgagtcta
     7861 cacttgaagt gacctcttta aagtatagca ccccgcccta cttactggaa gcagcaagtc
     7921 atctatcacc agaaaatttt gtggaagatg aagcaaagat atgtgtccag gaactacagt
     7981 gtcctaaaat tattaccaaa aattcacctc taccaagtag cattatctcc agaaaggaga
     8041 aaaatgatcc agtattcctg gaagaagcag ggaaagcaga aaaaattgtg atttccagat
     8101 ccacagcccc aactttcaac aaacaaacaa agagagttag ctggtcaagc tttaattctt
     8161 tgggacagta ttttactggt aaaataccga aggcaacacc tgagctcggg tcatcagaga
     8221 atagtgcctc tagtcctccc cgtttcaaaa cagagaagat ggaaagtaaa actgttttgc
     8281 ccttcactga taaatgtgaa tcctcaaatc tgacagtaaa cacatcgttt ggatcatgcc
     8341 ctcaatcaga aaccattatt tcatcattaa agattgatgc cacccttact agaaaaaatc
     8401 attctgaaca tccgctctgt tctgaagaag ccctggatcc agacattgaa ttgtctctag
     8461 taagtgaaga agataaacag tccacttcaa atagagattt tgaaacagaa gcagaagatg
     8521 aatctatcta ttttacacct gaactttacg atcctgaaga tacagatgaa gaaaaaaatg
     8581 acctagctga aactgataga ggaaatagat tggctaacaa ttcagattgc attttagcta
     8641 aagacctttt tgaaattaga actataaaag aagtagattc agccagagaa gtgaaagctg
     8701 aggattgcat agatacaaag ttgaatggaa ttctgcatat tgaagaaagt aaaattgatg
     8761 acattgatgg taatgtaaaa acaacttgga taaatgaact ggaactggga aaaactcatg
     8821 aaatagaaat aaagaacttt aaaccatctc cttccaaaaa taaaggcatg tttcctggtt
     8881 ttaaggcggc cgctggagga gactacaagg acgacgatga caagtcggcc gctggaggat
     8941 acccctacga cgtgcccgac tacgcctagg gccgcatcga gagatccggg agacacaacg
     9001 gtttccctct agcgggatca attccgcccc cccccctaac gttactggcc gaagccgctt
     9061 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     9121 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     9181 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     9241 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     9301 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     9361 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     9421 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     9481 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     9541 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc atggattcat
     9601 acctgctgat gtggggactg ctcacgttca tcatggtgcc tggctgccag gcagagctct
     9661 gtgacgatga cccgccagag atcccacacg ccacattcaa agccatggcc tacaaggaag
     9721 gaaccatgtt gaactgtgaa tgcaagagag gtttccgcag aataaaaagc gggtcactct
     9781 atatgctctg tacaggaaac tctagccact cgtcctggga caaccaatgt caatgcacaa
     9841 gctctgccac tcggaacaca acgaaacaag tgacacctca acctgaagaa cagaaagaaa
     9901 ggaaaaccac agaaatgcaa agtccaatgc agccagtgga ccaagcgagc cttccaggtc
     9961 actgcaggga acctccacca tgggaaaatg aagccacaga gagaatttat catttcgtgg
    10021 tggggcagat ggtttattat cagtgcgtcc agggatacag ggctctacac agaggtcctg
    10081 ctgagagcgt ctgcaaaatg acccacggga agacaaggtg gacccagccc cagctcatat
    10141 gcacaggtga aatggagacc agtcagtttc caggtgaaga gaagcctcag gcaagccccg
    10201 aaggccgtcc tgagagtgag acttcctgcc tcgtcacaac aacagatttt caaatacaga
    10261 cagaaatggc tgcaaccatg gagacgtcca tatttacaac agagtaccag gtagcagtgg
    10321 ccggctgtgt tttcctgctg atcagcgtcc tcctcctgag tgggctcacc tggcagcgga
    10381 gacagaggaa gagtagaaga acaatctaga ggatccggat tagtccaatt tgttaaagac
    10441 aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata
    10501 gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
    10561 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
    10621 aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga tggaacagct
    10681 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
    10741 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
    10801 ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag
    10861 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
    10921 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
    10981 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta
    11041 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
    11101 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcaca tgcagcatgt
    11161 atcaaaatta atttggtttt ttttcttaag tatttacatt aaatggccat agtacttaaa
    11221 gttacattgg cttccttgaa ataaacatgg agtattcaga atgtgtcata aatatttcta
    11281 attttaagat agtatctcca ttggctttct actttttctt ttattttttt ttgtcctctg
    11341 tcttccattt gttgttgttg ttgtttgttt gtttgtttgt tggttggttg gttaattttt
    11401 ttttaaagat cctacactat agttcaagct agactattag ctactctgta acccagggtg
    11461 accttgaagt catgggtagc ctgctgtttt agccttccca catctaagat tacaggtatg
    11521 agctatcatt tttggtatat tgattgattg a
//
