LOCUS       sequence-240752-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with EIN as a solubility and expression enhancing tag to 
            increase production of hard to express proteins such as AlkBH5 and 
            TEV cleaving site for the removal of the fusion tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Khatiwada B, Purslow JA, Underbakke ES, Venditti V
  TITLE     N-terminal fusion of the N-terminal domain of bacterial enzyme I 
            facilitates recombinant expression and purification of the human RNA
            demethylases FTO and Alkbh5.
  JOURNAL   Protein Expr Purif. 2019 Nov 15;167:105540. doi: 
            10.1016/j.pep.2019.105540.
  PUBMED    31740367
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2019-11-15"; volume: "167:105540. doi"; pages: 
            " 10.1016/j.pep.2019.105540"
COMMENT     Sequence Label: pET-EIN-AlkBH5(66-292)
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             778..798
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catcatcatc atcaccatag cagcatttca ggcattttag catccccggg tatcgctttc
       61 ggtaaagctc tgcttctgaa agaagacgaa attgtcattg accggaaaaa aatttctgcc
      121 gaccaggttg atcaggaagt tgaacgtttt ctgagcggtc gtgccaaggc atcagcccag
      181 ctggaaacga tcaaaacgaa agctggtgaa acgttcggtg aagaaaaaga agccatcttt
      241 gaagggcata ttatgctgct cgaagatgag gagctggagc aggaaatcat agccctgatt
      301 aaagataagc acatgacagc tgacgcagct gctcatgaag ttatcgaagg tcaggcttct
      361 gccctggaag agctggatga tgaatacctg aaagaacgtg cggctgacgt acgtgatatc
      421 ggtaagcgcc tgctgcgcaa catcctgggc ctgaagatta tcgacctgag cgccattcag
      481 gatgaagtca ttctggttgc cgctgacctg acgccgtccg aaaccgcaca gctgaacctg
      541 aagaaggtgc tgggtttcat caccgacgcg ggtggccgta cttcccacac ctctatcatg
      601 gcgcgttctc tggaactacc tgctatcgtg ggtaccggta gcgtcacctc tcaggtgaaa
      661 aatgacgact atctgattct ggatgccgta aataatcagg tttacgtcaa tccaaccaac
      721 gaagttattg ataaaatgcg cgctgttcag gagcaagtgg cttctgaaca tagcagcgaa
      781 aatctgtatt ttcagagc
//
