LOCUS       sequence-240753-         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with EIN as a solubility and expression enhancing tag to 
            increase production of hard to express proteins such as AlkBH5 and 
            TEV cleaving site for the removal of the fusion tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Khatiwada B, Purslow JA, Underbakke ES, Venditti V
  TITLE     N-terminal fusion of the N-terminal domain of bacterial enzyme I 
            facilitates recombinant expression and purification of the human RNA
            demethylases FTO and Alkbh5.
  JOURNAL   Protein Expr Purif. 2019 Nov 15;167:105540. doi: 
            10.1016/j.pep.2019.105540.
  PUBMED    31740367
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2019-11-15"; volume: "167:105540. doi"; pages: 
            " 10.1016/j.pep.2019.105540"
COMMENT     Sequence Label: pET-EIN-AlkBH5(66-292)
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccggaacgta gcgattatga agaacagcag ctgcagaaag aagaagaagc acgtaaagtt
       61 aaaagcggta ttcgtcagat gcgtctgttt agccaggatg aatgtgcaaa aattgaagcc
      121 cgtattgatg aagttgttag ccgtgcagaa aaaggcctgt ataatgaaca taccgttgat
      181 cgtgcaccgc tgcgcaataa atactttttt ggtgaaggtt atacctacgg tgcacagctg
      241 caaaaacgtg gtccgggtca agaacgtctg tatccgcctg gtgatgttga tgaaattccg
      301 gaatgggttc atcagctggt tattcagaaa ctggttgaac atcgtgttat tccggaaggt
      361 tttgttaata gcgcagtgat caatgattat cagcctggtg gttgtattgt gagccatgtt
      421 gatccgattc atatttttga acgtccgatt gttagcgtga gcttttttag cgatagcgca
      481 ctgtgttttg gttgcaaatt tcagtttaaa ccgattcgtg ttagcgaacc ggttctgagc
      541 ctgccggttc gtcgtggtag cgttaccgtt ctgagcggtt atgcagcaga tgaaattacc
      601 cattgtattc gtccgcagga tatcaaagaa cgtcgtgcag ttattattct gcgtaaaacc
      661 cgtctggatg caccgcgtct gtaa
//
